Comportamento quimiometabólico do músculo sóleo na fase aguda da imobilização articular

Abstract

O objetivo foi avaliar o perfil fisiológico do músculo sóleo na fase aguda da imobilização articular na posição de 90o. Ratos Wistar foram divididos em 4 grupos (n=6 cada): controle (C), imobilizado por 1 (Im1), 2 (Im2) e 3 dias (Im3). Após o período experimental, o músculo sóleo foi retirado e foram mensurados: o peso muscular, o índice de hidratação, a concentração de glicogênio e a concentração de DNA/proteínas totais. Os dados foram submetidos a análise estatística, com nível de significância fixado em p<0,05. No primeiro dia não houve alterações nas reservas glicogênicas, sendo observada redução progressiva das reservas: 53% no segundo dia e 65% no terceiro dia de imobilização. O peso muscular sofreu redução de 28,57% apenas no terceiro dia; o índice de hidratação aumentou 6,44% no segundo e 8,58% no terceiro dia. As concentrações de DNA tiveram elevação de 43,18% no primeiro dia, 59,09% no segundo e 75% no terceiro. Quanto à concentração de proteínas totais, houve elevação de 45,9% no primeiro dia, 32,25% no segundo e 58,95% no terceiro dia. Os resultados sugerem que a hipotrofia muscular é um processo desencadeado precocemente, envolvendo alterações quimiofisiológicas que são deflagradas na fase aguda da imobilização.

Perfil eletrocardiográfico e conteúdo glicogênico muscular de ratos tratados com nandrolona

“…Then, we evaluated the muscle glycogen reserves, and it was possible to see that the control group had a pattern of glycogen reserves that was similar to that mentioned in recent publications of the literature [25][26][27] . With respect to the group treated with nandrolone, as one may see in Figure 1, with the treatment, there changes only in the glycogen reserves of the ventricular chamber, with a reserve that was 127% greater than the control group's reserve.…”

Histomorphometric alterations of muscle soleus provoked by drawn out immobilization: experimental study with wistar lineage rats

“…The results reached with the histomorphometric analysis show and increase in the occupied space by the endomysium and perymisium from the animals from the experimental group from 21 and 45 days. Chingui et al (47) in their studies conclude that the biggest homeostatic compromising occurs in the initial phase of disuse. In this context, it is important to enhance that the determination of the results of studies on immobilization present a multifactorial factor differing regarding the model and used material to promote disuse, the period of immobilization, the articular position and the type of muscle fibers analyzed (27,28,30,36) .…”

Imobilização articular: efeitos sobre o tecido muscular de camundongos obesos e desnutridos

“…Thus, the anabolic effects of this hormone are reinforced by its anti-catabolic actions. However, insulin inhibits proteolysis, suppresses the release and inhibits oxidation of essential amino acids 20 . Given these characteristics, it is believed that animals from IOG and IMG groups suffered more severe damage to the muscle tissue because in addition to being affected by immobilization condition, usually when in obesity and protein malnutrition conditions, animals present disturbances in the mechanisms of supply and use of the insulin hormone, which enhances the muscle catabolic state.…”

“…Thus, muscle affected by these conditions may trigger an insulin resistance atrophy process 20 . Corroborating the findings of this research on protein malnutrition, experimental studies have shown that the offspring of rats exposed to this procedure showed a decrease in the area and diameter of the gastrocnemius and tibialis anterior muscle fibers 21 .…”