Multiplicação e enraizamento in vitro de Minirosa

Diniz, Josefa Diva Nogueira; Almeida, Jacqueline Leite; Oliveira, Alexandre Bosco de; Vidal, Francisco Ronaldo

doi:10.1590/s1806-66902014000100009

Cited by 3 publications

(4 citation statements)

References 12 publications

(6 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Evaluating the effect of BAP concentration, the greatest length was obtained with a BAP concentration of 2.0 mg L -1 which did not differ from concentrations of 0.0 and 3.0 mg L -1 but was greater than a concentration of 1.0 mg L -1 . In a study of micropropagation of miniature roses, Rosa chinensis Jacq, performed by Diniz et al (2014), an increased length of the aerials parts with a BAP concentration of 1.0 mg L -1 was obtained. Regarding the substrate used in the culture medium, coconut fiber produced length of aerial parts greater than vermiculite and agar.…”

Section: Resultsmentioning

confidence: 99%

Growth regulators and substrates for Oncidium baueri Lindl. micropropagation

Rodrigues

Nadal

Camargo

et al. 2016

SCA

View full text Add to dashboard Cite

An adequate concentration of growth regulators as well as the replacement of agar by an alternative medium may be promising from practical and financial points of view to produce orchid plants by micropropagation. The objective of this work was to evaluate different concentrations of growth regulator and alternative substrates for agar replacement in culture medium for in vitro multiplication and rooting of Oncidium baueri. In the explant multiplication phase, two experimental factors were evaluated-various concentrations of 6-benzylaminopurine (BAP) (0.0, 1.0, 2.0, and 3.0 mg L -1 ) and substrates (agar, vermiculite, and coconut fiber) added to MS medium. In the rooting phase, different concentrations of indole butyric acid (IBA) (0.0, 0.5, 1.0, and 1.5 mg L -1 ) were added to culture medium containing the same substrate. Six months after the experiments were initiated, the survival percentage, number of leaves, shoots, and roots and length of the aerial part and the major root were evaluated. The results suggested that addition of 1.0 mg L -1 BAP is necessary for the O. baueri in vitro multiplication phase, but IBA is not necessary in the rooting phase. For the substrate, vermiculite is not indicated as an agar replacement. In contrast, coconut fiber can be used in both multiplication and rooting phases of Oncidium baueri in vitro culture. Key words: Orchid. Coconut fiber. Culture medium. Vermiculite. ResumoNa micropropagação de orquídeas, a concentração adequada de reguladores de crescimento, bem como a substituição do ágar por substratos alternativos no meio de cultura podem ser promissoras do ponto de vista da praticidade e da economia financeira para a obtenção de mudas. Assim, o objetivo do trabalho foi avaliar diferentes concentrações de reguladores de crescimento e substratos alternativos em substituição ao ágar no meio de cultura para a multiplicação e o enraizamento in vitro de Oncidium baueri. Na fase de multiplicação dos explantes, foram avaliados dois fatores: concentração de benzilaminopurina (BAP) (0,0; 1,0; 2,0 e 3,0 mg L -1 ) e substrato (ágar, vermiculita e fibra de coco) no meio de cultura MS e, no período de enraizamento, foram testadas concentrações de ácido indolbutírico (AIB) (0,0; 0,5; 1,0

show abstract

Section: Resultsmentioning

confidence: 99%

Growth regulators and substrates for Oncidium baueri Lindl. micropropagation

Rodrigues

Nadal

Camargo

et al. 2016

SCA

View full text Add to dashboard Cite

show abstract

“…Dentro desta ampla diversidade, encontram-se as mini-roseiras (Rosa spp. ), popularmente chamadas de mini-rosas ou rosas-meninas, caracterizadas por flores de pequeno à médio porte, de florescimento contínuo e encontradas em diversas cores, tais como vermelho, rosa, amarelo, laranja, purpura e branca (Boettcher, 1991;Diniz, 2014).…”

Section: Introductionunclassified

“…As mini-roseiras são, geralmente, multiplicadas por métodos de propagação vegetativa tradicionais, como estaquia, mergulhia e enxertia (Diniz et al, 2014). No entanto, tais técnicas apresentam dependência sazonal, baixa taxa de multiplicação e favorecem a disseminação de pragas e doenças (Kumud et al 2015).…”

Section: Introductionunclassified

Assepsia de segmentos nodais de mini-rosa (Rosa sp.) para o estabelecimento in vitro

Silva

Costa

et al. 2019

J. Biotechnol. Biodivers.

View full text Add to dashboard Cite

O Brasil se destaca como um dos maiores produtores de plantas ornamentais do mundo. E as mini-rosas (Rosa sp.) estão entre as flores de vaso mais comercializadas. No entanto, apesar de sua grande importância econômica, os métodos de propagação tradicional possuem características indesejadas, pois favorecem a disseminação de doenças e pragas, dependência sazonal e baixa taxa de multiplicação. Portanto, o objetivo neste trabalho foi avaliar o uso de diferentes concentrações e tempos de imersão em solução de hipoclorito de sódio (NaClO) na assepsia de segmentos nodais de mini-rosa. Os segmentos nodais foram coletados de plantas jovens de mini-roseiras de cor vermelha, oriundas do campo. Foram avaliados nove tratamentos, combinando-se as concentrações de 0,75, 1,00 e 1,25% de NaClO e os tempos de 10, 20 e 30 minutos de exposição dos explantes ao agente desinfestante. O delineamento experimental utilizado foi o inteiramente casualizado, com nove tratamentos contendo 20 repetições. Ao longo de 30 dias de cultivo in vitro, avaliou-se o percentual de contaminação, sobrevivência e necrose dos explantes. De acordo com os dados obtidos, verificou-se alta taxa de contaminação em todos os tratamentos. Porém, os explantes submetidos à concentração de 1,25% de NaClO por 30 minutos, apresentaram a maior taxa de assepsia (45%). Ainda neste mesmo tratamento, não foram verificadas contaminações por bactérias nem ocorrência de necrose nos explantes. Portanto, os segmentos nodais de mini-rosa submetidos ao hipoclorito de sódio na concentração de 1,25% por 30 minutos de imersão obtiveram o maior percentual de assepsia.

show abstract

“…It is also influenced by the time of year and the low multiplication rate, which limit conventional propagation (Diniz et al, 2014). Some researchers have been successful in the multiplication and rooting of minirose explants in vitro with the aid of micropropagation, which allows rapid multiplication of species from a single individual, at any time of the year, with greater control over the health of the propagated material (Thakur & Karnosy, 2007).…”

Section: Introductionmentioning

confidence: 99%

Regional Substrates of the Brazilian Northeast on Acclimatization and Development Morphological of Minirose

Anjos¹,

Dias²,

Hernández³

et al. 2018

JAS

Self Cite

View full text Add to dashboard Cite

The transition from heterotrophism to autotrophism in acclimatization is a delicate process for most species. The objective of this work was to evaluate combinations of regional substrates of the Brazilian Northeast in the acclimatization and morphological development of minirose (Rosa chinensis 'Minima') seedlings. The experiment was carried out in a greenhouse using seedlings with 30 days of in vitro culture. The treatments were constituted by: 1) dry coconut powder; 2) 75% dry coconut powder + 25% rice husk; 3) 50% dry coconut powder + 50% rice husk; 4) 75% dry coconut powder + 25% charcoal rice husk; 5) 50% dry coconut powder + 50% carbonized rice husk; 6) 100% vermiculite; 7) 50% vermiculite + 50% dry coconut powder; 8) commercial substrate; 9) 75% sand + 25% dry coconut powder and 10) 50% sand + 50% dry coconut powder. After 21 days, the following characteristics were evaluated: survival percentage, leaf number, shoot height, dry matter of shoot and root and total dry matter. The regional materials tested showed a good choice of substrates to be used in the acclimatization of minirose seedlings. The vermiculite presented the worst results in the characteristics evaluated. Dry coconut powder as substrate should be used in mixtures with other materials to provide better acclimatization conditions. The use of 50% dry coconut powder + 50% rice husk provided a best development and growth of minirose seedlings (Rosa chinensis 'Minima') than other tested substrates

show abstract

Multiplicação e enraizamento in vitro de Minirosa

Cited by 3 publications

References 12 publications

Growth regulators and substrates for Oncidium baueri Lindl. micropropagation

Growth regulators and substrates for Oncidium baueri Lindl. micropropagation

Assepsia de segmentos nodais de mini-rosa (Rosa sp.) para o estabelecimento in vitro

Regional Substrates of the Brazilian Northeast on Acclimatization and Development Morphological of Minirose

Contact Info

Product

Resources

About