Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients

Soares, Rosilene Calazans; Oliveira, Márcio Campos; Souza, Lélia Batista de; Costa, Antônio de Lisboa Lopes; Pinto, Leão Pereira

doi:10.1590/s1678-77572008000500007

Cited by 14 publications

(11 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our samples without cancer were diagnosed as traumatic fibromas, and therefore the high prevalence of HPV in our group without cancer must be a regional characteristic. Brazilian (44,46) and Italian (14) studies of OSCC have reported a higher prevalence of HPV18 than HPV16, which agrees with our results, but does not agree with the majority of studies conducted in other countries where HPV16 is the most prevalent type in oral malignant lesions (16,41,45). Only the OSCC WT and OSCC W groups presented co-infection with both viral types, representing 10% of our malignant oral lesions, which is consistent with data reported by other authors (41,44,47).…”

Section: Discussionsupporting

confidence: 79%

BUBR1 expression in benign oral lesions and squamous cell carcinomas: Correlation with human papillomavirus

Soares¹

2010

Oncol Rep

View full text Add to dashboard Cite

Abstract. Oral squamous cell carcinoma (OSCC) is the most common head and neck cancer. Only in Brazil, the estimate is for 14,160 new cases in 2009. HPV is associated with increasing risk of oral cancer, but its role in carcinogenesis is still controversial. BUBR1, an important protein in the mitotic spindle assembly checkpoint (SAC), has been associated with some virus-encoded proteins and cancer. The aim of the present study was to evaluate the expression of BUBR1 in non-malignant oral lesions and OSCC with and without metastasis associated with HPV infection. We performed immunohistochemistry for BUBR1 in 70 OSCC biopsies divided into three groups (in situ tumors, invasive tumors without metastasis and invasive tumors with metastasis) with their respective lymph nodes from samples with metastasis and in 16 non-malignant oral lesions. PCR was performed in order to detect HPV DNA. Significantly higher BUBR1 expression associated with shorter survival (p=0.0479) was observed in malignant lesions. There was also a significant correlation (r=1.000) with BUBR1 expression in lesions with metastasis and their lymph nodes. Ninety percent of OSCC and 100% of benign lesions were HPV positive. HPV16 and HVP18 were present in 13 and 24% of HPV-positive OSCC samples, respectively. HPV was more prevalent (76%) in samples with a high BUBR1 expression and the absence of viral DNA had no influence on BUBR1 expression. These findings suggest that HPV could be associated with overexpression of BUBR1 in OSCC, but not in benign oral lesions.

show abstract

Section: Discussionsupporting

confidence: 79%

BUBR1 expression in benign oral lesions and squamous cell carcinomas: Correlation with human papillomavirus

Soares¹

2010

Oncol Rep

View full text Add to dashboard Cite

show abstract

“…Soares et al (34), found that 11 out of 33 cases (33.33%) were positive for HPV18 (81.81%) or HPV16 (18.19%). Of these, p21 expression was positive in 5 of 11 cases(45.45%); they found no statistically significant association.…”

Section: P21waf1/cip1 Expression In Precancerous Lesions and The Rolementioning

confidence: 99%

The role of p21Waf1/CIP1 as a Cip/Kip type cell-cycle regulator in oral squamous cell carcinoma (Review).

Pérez‐Sayáns¹,

Suárez-Peñaranda²,

Díz³

et al. 2013

Med Oral

View full text Add to dashboard Cite

Oral Squamous Cell Carcinoma (OSCC) is biologically characterized by the accumulation of multiple genetic and molecular alterations that end up clinically characterized as a malignant neoplasm through a phenomenon known as multistep. The members of the Cip/Kip family, specifically p21Waf1/CIP1, are responsible for cell cycle control, blocking the transition from phase G1 to phase S. We made a search of articles of peer-reviewed Journals in PubMed/ Medline, crossing the keywords. The goal of this paper is to determine the relationship between p21Waf1/CIP1 expression and several clinical and pathological aspects of OSCC, their relationship with p53 and HPV, as well as genetic alterations in their expression pattern, their use as a prognosis market in the evolution of precancerous lesions and their roles in anticancer treatments. The results of p21WAF1/CIP1 expression in OSCC showed mixed results in terms of positivity/negativity throughout different studies. It seems that, although p21Waf1/CIP1 expression is controlled in a p53-dependent manner, coexpression of both in OSCC is not intrinsically related. Although the presence of HPV viral oncoproteins increases p21Waf1/CIP1 levels, the small number of studies, have forced us to disregard the hypothesis that HPV infected lesions that present better prognosis are due to a p21Waf1/CIP1–dependent control. The role of p21WAF1/CIP1 as cell-cycle regulator has been well described; however, its relationship to OSCC, the clinical and pathological variables of tumors, HPV and different treatments are not entirely clear. Thus, it would be very interesting to pursue further study of this protein, which may have a significant value for the diagnosis, prognosis and therapy of this type of tumors. Key words:p21Waf1/CIP1, Cip/Kip type cell-cycle regulator, Oral squamous cell carcinoma (OSCC), p53, genetic alterations.

show abstract

“…Lira (2010) 25 Mean of 65 y ------49/26 Oliveira (2008) 26 Mean of 59 y 81.6% ---73/14 Simonato (2008) 27 79.3% under 60 y 89.7% 62.1% 27/2 Soares (2008) 28 Mean of 65 y ---------Oliveira (2009) 29 59% over 60 y ------57/31 Pereira (2011) 30 Mean of 63 y ------19/8 Spindula-Filho (2011) 31 55.3% over 65 y 72.3% 61.7% 34/13 Cordeiro-Silva (2012) 32 44.5% over 58 y 80.0% 68.9% 35/10 Kaminagakura (2012) 33 Mean of 51 y 84.0% 62.0% 81/33 Marques-Silva (2012) 34 33 38 Mean of 45 y ------21/43 Ribeiro (2014) 39 Mean of 32 y 27.4% ---31/31 Vidotti (2014) 40 42 Mean of 29 y ------30/0 Peixoto (2011) 43 Mean of 37 70 3 (4.3%) ---------Sacramento (2006) 6 50 7 (14.0%) 1 (2.0%) 1 (2.0%) 2 (4.0%) Silva (2007) 16 10 1 (10.0%) ---------Esquenazi (2010) 7 100 0 (0.0%) ---------Horewicz (2010) 8 104 0 (0.0%) 0 (0.0%) ------Araujo (2011) 45 16 1 (6.2%) 0 (0.0%) 0 (0.0%) 0 (0.0%) Betiol (2012) 13 22 16 16 (100.0%) ---------Mazon (2011) 17 18 6 (33.0%) 0 (0.0%) 0 (0.0%) 0 (0.0%) Fregonezi (2012) 23 Oral/oropharyngeal HPV infection in patients with oral or oropharyngeal SCC Miguel (1998) 24 28 4 (14.3%) ---------Soares (2002) 18 10 0 (0.0%) ---------Soares (2003) 19 27 13 (48.1%) ------10 (37.0%) Fregonesi (2003) 20 17 6 (35.3%) ------5 (29.0%) Cortezzi (2004) 15 16 4 (25.0%) 4 (25.0%) ------Silva (2007) 16 50 37 (74.0%) ---------Soares (2007) 27 29 5 (17.2%) --------...…”

Section: Comparative Studies (Benign Lesions Versus Scc Patients)mentioning

confidence: 99%

Prevalence of oral and oropharyngeal human papillomavirus infection in Brazilian population studies: a systematic review

Matos

Miranda

Cernea

2015

Brazilian Journal of Otorhinolaryngology

View full text Add to dashboard Cite

show abstract

Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients

Cited by 14 publications

References 19 publications

BUBR1 expression in benign oral lesions and squamous cell carcinomas: Correlation with human papillomavirus

BUBR1 expression in benign oral lesions and squamous cell carcinomas: Correlation with human papillomavirus

The role of p21Waf1/CIP1 as a Cip/Kip type cell-cycle regulator in oral squamous cell carcinoma (Review).

Prevalence of oral and oropharyngeal human papillomavirus infection in Brazilian population studies: a systematic review

Contact Info

Product

Resources

About