Histological analysis of the biocompatibility of three different calcium hydroxide-based root canal sealers

Veloso, Heloísa Helena Pinho; Santos, Roberto Alves dos; Araújo, Túlio Pessoa de; Leonardi, Denise Piotto; Baratto‐Filho, Flares

doi:10.1590/s1678-77572006000500014

Cited by 12 publications

(16 citation statements)

References 13 publications

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“…In this study, after 7 days, all sealers induced similar inflammatory reactions in the rat connective tissue, with most specimens showing moderate to severe chronic inflammation and presence of foreign-body multinucleated giant cells. Similar results were found by Veloso et al (10) for Sealapex. However, some authors have observed that Sealapex induced a mild to moderate inflammation in the subcutaneous tissue after 7 days (11,12).…”

Section: Discussionsupporting

confidence: 92%

Biocompatibility of sealers used in apical surgery: a histological study in rat subcutaneous tissue

et al. 2011

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The aim of this study was to evaluate the biocompatibility of sealers used in apical surgery in rat subcutaneous tissue. Sterile polyethylene tubes were filled with the following sealers: Sealapex, Sealapex with addition of zinc oxide, Sealer 26, Sealer 26 with thicker consistency (greater powder-to-resin ratio) and White MTA. The tubes were implanted in the dorsum of male rats and after 7, 21 and 42 days, the animals were killed, obtaining 5 specimens for each sealer in each evaluation period. The lateral surface of the tube was used as negative control. The inflammatory reaction to contact with the sealers was classified as absent, mild, moderate and severe. At 7 days, all sealers caused similar inflammatory reactions in the connective tissue of the animals, with most specimens presenting a moderate to intense chronic inflammatory reaction, with presence of multinucleated giant cells. At 21 days, Sealer 26 and Sealer 26 with thicker consistency presented more intense inflammatory reaction (p=0.004), whereas after 42 days, the inflammatory reaction ranged from absent to mild with statistically similar results for both materials (p=0.08). Except for MTA, all sealers presented foreign-body granulomatous reaction at 42 days. All sealers but Sealapex presented a statistically significant decrease of the inflammatory reaction over time. In conclusion, all sealers caused moderate to severe inflammation in the earlier evaluation period. However, Sealer 26 and Sealer 26 with thicker consistency caused more intense inflammatory reactions after 21 days of contact with the tissues and no granulomatous reaction was observed for MTA at the final period of analysis.

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Section: Discussionsupporting

confidence: 92%

Biocompatibility of sealers used in apical surgery: a histological study in rat subcutaneous tissue

et al. 2011

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“…When compared against each other, tissues surrounding Sealapex displayed a progressive reduction in the numbers of inflammatory cells over the period of 60 days, and tissue surrounding Apexit showed more aggressive reactions compared to Sealapex. Tissues adjacent to Sealer 26 continued to show high numbers of neutrophils (initial stages) and macrophages (later stages) compared with Apexit and Sealapex (80). In a scanning x-ray microanalysis of root canal sealers, it was found that a calcium-containing sealer exhibited less toxicity than one containing lead or magnesium (eg, N2 apical and RC-2B) (81).…”

Section: Biocompatibilitymentioning

confidence: 99%

Calcium Hydroxide–Based Root Canal Sealers: A Review

Desai

Chandler

2009

Journal of Endodontics

164

128

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“…In the present study, in 2 days all groups showed a severe inflammatory response and absence of tissue repair. These results can be explained by the surgical trauma 19 . In 3 days, the inflammatory response was reduced and there was absence of tissue repair in all groups.…”

Section: Discussionmentioning

confidence: 86%

The biocompatibility of a new endodontic paste used in dental trauma

Soares

Prado

Brazão

et al. 2015

Rev. odontol. UNESP

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<title>Abstract</title><sec><title>Objective</title>This study investigated the short-term subcutaneous tissue reaction of a new endodontic paste, based on the association among calcium hydroxide [Ca(OH)2], 2% chlorhexidine gel (CHX) and zinc oxide (ZnO) in comparison with a control paste, and the individual substances alone.</sec><sec><title>Material and method</title>Twenty-four male wistar rats were used. Polyethylene tubes containing the materials were implanted into the dorsal connective tissue of rats. Empty implanted tubes served as controls. The materials used were: Ca(OH)2+CHX+ZnO paste in two different proportions (2:1:2) and (2:1:3), a control paste, Ca(OH)2+CHX (2:1), and the substances alone. After time intervals of 2, 3, 7 and 15 days, the animals were euthanized and the specimens prepared for histological processing. Data were evaluated qualitatively according to the levels of tissue repair and the degree of inflammation.</sec><sec><title>Result</title>On the second day all the materials were observed to cause a severe inflammatory response. On the third day, the pastes Ca(OH)2+CHX+ZnO (2:1:2) and (2:1:3) showed reduction in degree of inflammation. On seventh day the tissue was already more organized with presence of collagen fibers in all materials. After 15 days it was found that compound Ca(OH)2+CHX+ZnO (2:1:2) showed areas with no inflammatory infiltrate.</sec><sec><title>Conclusion</title>The experimental pastes, Ca(OH)2+CHX+ZnO in two different proportions (2:1:2) and (2:1:3), showed favorable results with respect to biocompatibility, when evaluated for a short-term.</sec>

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Histological analysis of the biocompatibility of three different calcium hydroxide-based root canal sealers

Cited by 12 publications

References 13 publications

Biocompatibility of sealers used in apical surgery: a histological study in rat subcutaneous tissue

Biocompatibility of sealers used in apical surgery: a histological study in rat subcutaneous tissue

Calcium Hydroxide–Based Root Canal Sealers: A Review

The biocompatibility of a new endodontic paste used in dental trauma

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