Qualitative parameters of the piapara semen (Leporinus elongatus Valenciennes, 1850)

Jr, Danilo Streit; Rn, Sirol; Rp, Ribeiro; Gv, Moraes; Ld, Vargas; Watanabe, André Luiz

doi:10.1590/s1519-69842008000200019

Cited by 23 publications

(13 citation statements)

References 3 publications

(2 reference statements)

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“…These tests are implemented in the PROC GLM and PROC GENMOD procedures, respectively. MiraLópez et al (2010) for the same specie (89.0%) and for other species (Streit Jr et al 2008;Povh et al 2010;Maria et al 2011;Streit Jr et al 2012). In addition, no reference range of sperm motility was available for L. marmoratus in Brazil.…”

Section: Methodsmentioning

confidence: 89%

“…Sperm count was determined by diluting 5.0 µL of the semen sample in 25 mL buffered formal saline (1:5000) and spermatozoa were counted in a Neubauer chamber with 10 µL diluent (semen + buffered formal saline) under a light microscope (20X). Sperm morphology was evaluated in the smears of the diluents (semen + buffered formal saline) stained with Rose Bengal as described by Streit Jr et al (2008). The microscope slide containing the smears was dried and examined under a phase-contrast microscope at 40X magnification.…”

Section: Methodsmentioning

confidence: 99%

“…A significant difference was only in the frequency of loose heads, which was lower in the treatments with 0.4 and 0.6 Ovopel pellet/kg live weight (10.32 and 41.67%, respectively). A number of studies have shown high frequencies of secondary sperm defects in fish, particularly loose heads and tails (Streit Jr et al 2008;Maria et al 2011). These high frequencies of secondary abnormalities might be related to the procedure of smear preparation or semen collection, environmental temperature, diseases, animal feeding and sperm duct problems (Herman et al 1994).…”

Section: Methodsmentioning

confidence: 99%

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Ovopel and Carp Pituitary Extract as Spawning Inducers in Males of the Amazon Catfish Leiarius marmoratus (Gill, 1970)

Araújo

Ribeiro

et al. 2014

Braz. arch. biol. technol.

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Section: Methodsmentioning

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Ovopel and Carp Pituitary Extract as Spawning Inducers in Males of the Amazon Catfish Leiarius marmoratus (Gill, 1970)

Araújo

Ribeiro

et al. 2014

Braz. arch. biol. technol.

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“…Other studies report 90% (KAVAMOTO et al, 1999) and 97% (MILIORINI et al, 2011) normal sperm in Prochilodus lineatus, 64% in Leporinus elongatus (STREIT Jr. et al 2008b), 57% in the dorado Salminus maxillosus (STREIT Jr. et al 2008a), 53% in the cachara Pseudoplatystoma reticulatum (STREIT Jr. et al 2012) and 16% in the Nile tilapia Oreochromis niloticus (MATAVELI et al 2007). The variation of normal semen percentage found among the species is influenced by several factors such as the nutritional status of the fish, size, age, and time of the year (VIVEIROS; GODINHO, 2009;SANCHES et al, 2011;NAVARRO et al, 2014).…”

Section: Discussionmentioning

confidence: 95%

Semen characteristics of the amazonian jundiá (Leiarius marmoratus Gill, 1870)

Navarro

Pivato²

2016

Biosci. J.

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ABSTRACT:The study evaluated qualitative and quantitative characteristics of Amazonian jundiá semen. The experiment was carried out in February 2015 at Cia do Peixe, a company located in Goiás state, Brazil, that specialises in fish fry production. Three male L. marmoratus farmed in dugout ponds, weighing 1.34 ± 0.29 kg and measuring 39.33 ± 1.15 cm in length, were tested. The specimens were captured and transferred to a 1000-L tank at the Reproduction Laboratory of the abovementioned company. Water temperature was measured at 7:00 a.m. and 5:00 p.m., and the fish were fed twice daily. For semen collection, the fish were captured from the tank using a dip net and constrained with a wet cotton towel. Their eyes were covered and their urogenital papilla was cleaned and dried with paper towel. Semen was collected in microcentrifuge tubes that were immersed in ice and analysed immediately. On average, the sperm motility rate was 83.33%, motility duration was 5.33 min, sperm concentration was 1,795,833 cells/ mm 3 , and vigour was 4. The morphological analysis of the sperm revealed tail anomalies in 17.66%, midpiece anomalies in 20%, and head anomalies in 5.33%. Based on qualitative and quantitative characteristics, L. marmoratus semen was shown to be suitable for use in artificial reproduction procedures.

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“…Porém, estes autores descreveram que os percentuais elevados deste tipo de anormalidades podem estar relacionados ao procedimento de preparação e coleta do sêmen (Streit-Jr et al, 2008).…”

Section: Resultsunclassified

QUALIDADE DE GAMETAS COM A TÉCNICA DE ENDOSCOPIA EM CURIMBA (Prochilodus lineatus)

Andrade¹,

Ferreira²,

Paula³

et al. 2012

AVS

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RESUMO:A endoscopia pode facilitar o manejo em piscicultura e possibilita a realização da sexagem de forma precisa, mesmo que seu uso ainda seja restrito. A curimba é uma espécie de peixe reofílica, utilizada em experimentos como espécie modelo para peixes migradores. Este trabalho teve como objetivo viabilizar a utilização do endoscópio rígido na sexagem de reprodutores de curimba e determinar se a técnica altera os índices reprodutivos. Foram examinados 16 curimbas, nos quais foi identificado o sexo através da introdução do endoscópio pelo poro urogenital para visualização das gônadas. O endoscópio rígido utilizado (modelo Multi-Purpose Rigid™, Karl Storz, Germany) apresentava ângulo de 30º, com 2,7 mm de diâmetro e 18 cm de comprimento. Foi realizada a indução hormonal 20 dias após a aplicação da técnica, as fêmeas receberam duas doses de extrato bruto de hipófise de carpa (EBHC) de 0,4 e 4,0 mg/kg de peixe, com intervalo de 12 horas e os machos receberam uma única dose de 4,0 mg/kg de peixe de EBHC, no momento da segunda aplicação nas fêmeas. Após 12 horas, procedeu-se a extrusão dos gametas, sendo retirada uma amostra de sêmen para avaliação da morfologia, taxa (%) e duração (segundos) da motilidade e concentração espermática. Para as fêmeas realizou-se a avaliação do peso da desova e coleta de uma amostra dos ovócitos para avaliar a posição da vesícula germinativa e o diâmetro dos ovócitos. Pode-se observar que 100% dos machos espermiaram e 62% das fêmeas desovaram. Além disso, todos os índices reprodutivos foram considerados dentro dos padrões esperados para a espécie. Conclui-se que a endoscopia pode ser utilizada de maneira eficiente na sexagem e na avaliação do grau de maturação gonadal, antes da piracema, sem alterar a eficiência reprodutiva dos peixes. Palavras-chave: endoscópio; peixe; reprodução QUALITY OF GAMETES WITH ENDOSCOPY TECHNIQUE IN CURIMBA (PROCHILODUS LINEATUS)ABSTRACT: Endoscopy can facilitate for fish and allows the performance of sexed accurately, even if its use is still restricted. The curimba is a rheophilic species of fish, used in experiments as a model species for migratory fish. This study aimed to enable the use of the endoscope in sexing breeding curimba and determine whether the technique alters the reproductive rates. Curimbas 16 were examined, in which sex was identified by the introduction of the pore urogenital endoscope for viewing the gonads. The endoscope used (model Multi-Purpose Rigid ™, Karl Storz, Germany) had a 30° angle with 2.7 mm diameter and 18 cm in length. Hormonal induction was performed 20 days after application of the technique, the females received two doses of crude extract of carp pituitary (CECP) of 0.4 and 4.0 mg / kg of fish, with an interval of 12 hours and the males receiving a single dose of 4.0 mg / kg of fish CECP, when the second application in females. After 12 hours, we proceeded to the extrusion of the gametes, which was removed a semen sample for evaluation of morphology, rate (%) and duration (seconds) motility and sperm concentration. F...

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Qualitative parameters of the piapara semen (Leporinus elongatus Valenciennes, 1850)

Cited by 23 publications

References 3 publications

Ovopel and Carp Pituitary Extract as Spawning Inducers in Males of the Amazon Catfish Leiarius marmoratus (Gill, 1970)

Ovopel and Carp Pituitary Extract as Spawning Inducers in Males of the Amazon Catfish Leiarius marmoratus (Gill, 1970)

Semen characteristics of the amazonian jundiá (Leiarius marmoratus Gill, 1870)

QUALIDADE DE GAMETAS COM A TÉCNICA DE ENDOSCOPIA EM CURIMBA (Prochilodus lineatus)

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