“…133 Migratory species such as the C. macropomun are usually induced with the same standardized CPE protocol of 5.5 mg kg -1 body weight, in two applications (10% and 90%, with a 12-h interval between applications) of fractionated doses of pituitary extracts, which have been used for many years. [134][135][136][137][138][139] One of the best-studied species is P. mesopotamicus has also been induced with hypophysation, but together with prostaglandin administration to give excellent results 79,82,140 and increased the ovulation rate. However, while in many marine and freshwater species of the northern hemisphere, such as common carp, hypophysation has been replaced by synthetic products, such as the use of GnRH or the Linpe method combining GnRH with a dopamine inhibitor (domperidone, metoclopramide or pimozide), 11,18,23 there have been inconsistent results and few successful studies with the use of synthetic products in native LA fish.…”