Knowledge of insect diversity in Brazil: challenges and advances

Rafael, José Albertíno; Aguiar, Alexandre P.; Amorim, Dalton de Souza

doi:10.1590/s1519-566x2009000500001

Cited by 28 publications

(22 citation statements)

References 11 publications

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“…It is important to point out that, concerning tungid fleas, a large part of samples preserved in scientific collections is constituted by neosomes removed from several sites of their hosts (Figures 1, 2). Although some museums do not allow dissection of type specimens due to the risks of damage [24], sometimes it is necessary to dissect neosomes for identification, since that a dissected specimen is not a damaged specimen. Among siphonapterids, many species are morphologically indistinguishable (eg, some female fleas Rhopalopsyllidae) or have their identification difficult due to loss of structures (eg, loss of legs and bristles in Tungidae, during the formation of the neosome) [3,21].…”

Section: Resultsmentioning

confidence: 99%

Use of Multiple Displacement Amplification as Pre-polymerase Chain Reaction (Pre-PCR) to amplify genomic DNA of siphonapterids preserved for long periods in scientific collections

Avelar

Linardi

2010

Parasites Vectors

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The recently developed Multiple Displacement Amplification technique (MDA) allows for the production of a large quantity of high quality genomic DNA from low amounts of the original DNA. The goal of this study was to evaluate the performance of the MDA technique to amplify genomic DNA of siphonapterids that have been stored for long periods in 70% ethanol at room temperature. We subjected each DNA sample to two different methodologies: (1) amplification of mitochondrial 16S sequences without MDA; (2) amplification of 16S after MDA. All the samples obtained from these procedures were then sequenced. Only 4 samples (15.4%) subjected to method 1 showed amplification. In contrast, the application of MDA (method 2) improved the performance substantially, with 24 samples (92.3%) showing amplification, with significant difference. Interestingly, one of the samples successfully amplified with this method was originally collected in 1909. All of the sequenced samples displayed satisfactory results in quality evaluations (Phred ≥ 20) and good similarities, as identified with the BLASTn tool. Our results demonstrate that the use of MDA may be an effective tool in molecular studies involving specimens of fleas that have traditionally been considered inadequately preserved for such purposes.

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Section: Resultsmentioning

confidence: 99%

Use of Multiple Displacement Amplification as Pre-polymerase Chain Reaction (Pre-PCR) to amplify genomic DNA of siphonapterids preserved for long periods in scientific collections

Avelar

Linardi

2010

Parasites Vectors

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“…All seven species previously recorded from that state were also found during this study. Lewinsohn & Prado (2005) stated that there is a big problem in Brazil concerning the lack of knowledge on the biodiversity of most groups, and Rafael et al (2009) concluded that the problem is due to the combination of enormous species richness on one hand and the small number of taxonomists able to recognize and describe this wealth of species.…”

Section: Discussionmentioning

confidence: 99%

Diversity and distribution of jumping plant-lice (Hemiptera: Psylloidea) along edges of Amazon–Cerrado transitional forests in Sorriso, Mato Grosso, Brazil

Mazzardo¹,

Barreto²,

Queiroz³

et al. 2017

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Abstract.Little is known about the jumping plant-lice of Brazil from where seven families, 45 genera and 76 species have been previously reported, but estimates suggest that there may be as many as 1,000 species. This study reports 34 species of Psylloidea which were collected along the edges of Amazon-Cerrado natural transitional forests in the municipality of Sorriso, state of Mato Grosso, from August 2013 to July 2014. Of the species reported in this study only nine represent described taxa, two of which are reported for the first time from Mato Grosso.

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“…Insects are the most diverse of all animal classes on the planet, and Brazil is the country with the greatest insect diversity, with estimates of around 400-500 thousand known species and most of these inhabiting the Amazon forest [1–3]. Anthropic activity has affected some insect species, especially dipterian vectors such as sand flies, biting midges, and mosquito populations [4–6].…”

Section: Introductionmentioning

confidence: 99%

Diversity of mosquitoes (Diptera: Culicidae) collected in different types of larvitraps in an Amazon rural settlement

Almeida

Belchior

Ríos-Velásquez

et al. 2020

Preprint

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Anthropogenic environments provide favorable conditions for some species, which are especially suitable for mosquitoes that present eclecticism at the moment of choice for the site of oviposition. The present study, the diversity of mosquitoes in plastic container, bamboo internode, and tire placed in forest, the forest edge, and peridomicile environments in a rural settlement area was assessed. Eighteen sampling points were chosen, delimited by a buffer 200 m, placed in environments: forest, forest edge, and peridomicile. In each environment, larvitraps were installed, separated by a minimum distance of 7 m and 1 m from the ground. A total of 10,131 immature mosquitoes of 20 species were collected. The most abundant species was Culex urichii (29.5%), followed by Trichoprosopom digitatum (27.1%), and Cx. (Melanoconion) sp. (10,4%). There was a difference in the composition of immature mosquito populations between larvitraps (p<0.0005), and the plastic container hosted a greater diversity of species, whereas tires presented a greater abundance of individuals. The forest, forest edge, and peridomicile environments were also significantly different with regard to diversity of immature mosquito populations (p<0.0010). The forest edge was the environment with the greatest diversity of species, followed by the peridomicile and forest environments. In the forest and peridomicile, plastic container larvitraps had the greatest diversity, whereas the forest edge tire presented the largest number of individuals. Further, tire larvitraps collected the largest number of individuals in all environments. We identified 10 species associated with the bamboo internode and tire. We also observed the preference of species for artificial larvitraps, such as the plastic container and tire, even in wild environments. These artificial objects may represent a risk factor for the population living in this region, as all vector species found in the study were present in plastic containers and tires.

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Knowledge of insect diversity in Brazil: challenges and advances

Cited by 28 publications

References 11 publications

Use of Multiple Displacement Amplification as Pre-polymerase Chain Reaction (Pre-PCR) to amplify genomic DNA of siphonapterids preserved for long periods in scientific collections

Use of Multiple Displacement Amplification as Pre-polymerase Chain Reaction (Pre-PCR) to amplify genomic DNA of siphonapterids preserved for long periods in scientific collections

Diversity and distribution of jumping plant-lice (Hemiptera: Psylloidea) along edges of Amazon–Cerrado transitional forests in Sorriso, Mato Grosso, Brazil

Diversity of mosquitoes (Diptera: Culicidae) collected in different types of larvitraps in an Amazon rural settlement

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