Carbaryl degradation by bacterial isolates from a soil ecosystem of the Gaza Strip

Hamada, Mazen; Matar, Ammar; Bashir, Abdallah

doi:10.1590/s1517-838246420150177

Cited by 30 publications

(10 citation statements)

References 18 publications

(20 reference statements)

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“…On the basis of this, more tolerant strain may be have some ability to degrade or resistance against this organophosphate, but some strains have shown growth inhibition zone at 2 or 4 or 6 ll/ml, and these types of strains may not have the ability to degrade the pesticides. These strains have no inhibition zone and grow well on a media plate that strains may be degraded the pesticide and used as a sole source of carbon for their growth and development (Verma et al 2014;Hamada et al 2015;Jadhav and David 2016).…”

Section: Effect Of Chlorpyrifos Pesticide On Growth Inhibition Of Micmentioning

confidence: 99%

Screening of bacterial strains for developing effective pesticide-tolerant plant growth-promoting microbial consortia from rhizosphere soils of vegetable fields of eastern Uttar Pradesh, India

Verma

Jaiswal

Maurya

2016

Energ. Ecol. Environ.

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The current study was intended to isolate and characterize the plant growth-promoting properties and pesticide (chlorpyripfos) tolerance ability of microbial strains for sustainable vegetable productions. Plant growthpromoting microorganism is a group of microbial consortia to improve crop growth and yield by various direct mechanisms, e.g. nitrogen fixation, phosphate solubilization, production of plant growth hormones, ammonia and siderophore, and indirect mechanisms, e.g. production of antibiotic, hydrogen cyanide to help as biocontrolling agent. Totally, 50 microbes were isolated from soils of vegetable field. Out of 50, 14 strains were selected on the basis of morphological, growth biochemically characters (e.g. gram staining, amylase, cellulase, catalase and citrate test) and plant growth-promoting properties. All strains have ability of ammonia production. Out of 14 strains, IESDV2, IESDV3, IESDV4, IESDV11, IESDV12 and IESD28 were found significant increase in production of IAA, ammonia and phosphate solubilization than others. Strains IESDV3 and IESDV11 showed tolerant at 6 ll/ml concentration of chlorpyrifos which is three-time increase concentration of recommended dose (2 ll/ml). Others strains IESDV12, IESDV13 and IESDV28 were also showed resistance or tolerance at different concentrations of pesticide 2, 4, 6 and 20 ll/ml. These strains also showed plant growth-promoting rhizobacteria activities. Therefore, the main result of this study is to screen and identify that three strains (IESDV3, IESDV11, IESDV12 and IESDV28) can be used as effective pesticide-tolerant plant growth-promoting microbial consortia for vegetable production under sustainable agriculture at eastern Uttar Pradesh.

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Section: Effect Of Chlorpyrifos Pesticide On Growth Inhibition Of Micmentioning

confidence: 99%

Screening of bacterial strains for developing effective pesticide-tolerant plant growth-promoting microbial consortia from rhizosphere soils of vegetable fields of eastern Uttar Pradesh, India

Verma

Jaiswal

Maurya

2016

Energ. Ecol. Environ.

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“…Rhodococcus, Arthrobacter, Micrococcus, Burkholderia, and Pseudomonas spp. have been reported to degrade carbaryl as a sole source of carbon and energy (16)(17)(18)(19)(20)(21). However, the metabolic fate of carbaryl as a nitrogen source has not been studied in these organisms.…”

mentioning

confidence: 99%

Carbaryl as a Carbon and Nitrogen Source: an Inducible Methylamine Metabolic Pathway at the Biochemical and Molecular Levels in Pseudomonas sp. Strain C5pp

Kamini

Sharma

Punekar

et al. 2018

Appl Environ Microbiol

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Carbaryl is the most widely used carbamate family pesticide, and its persistent nature causes it to pollute both soil and water ecosystems. Microbes maintain the Earth’s biogeochemical cycles by metabolizing various compounds present in the matter, including xenobiotics, as a sole source of carbon, nitrogen, and energy. Soil isolatePseudomonassp. strain C5pp metabolizes carbaryl efficiently as the carbon source. Periplasmic carbaryl hydrolase catalyzes the conversion of carbaryl to 1-naphthol and methylamine. 1-Naphthol was further used as a carbon source via gentisate, whereas the metabolic fate of methylamine is not known. Here, we demonstrate that strain C5pp showed efficient growth on carbaryl when supplied as a carbon and nitrogen source, suggesting that the methylamine generated was used as the nitrogen source. Genes involved in the methylamine metabolism were annotated and characterized at the biochemical and molecular level. Transcriptional and enzyme activity studies corroborate that the γ-glutamylmethylamide/N-methylglutamate (GMA/NMG) pathway is involved in the metabolism of carbaryl and methylamine as a nitrogen source. Compared to carbaryl, methylamine was found to be an effective inducer for the metabolic and transporter genes. Strain C5pp also harbored genes involved in sarcosine metabolism that were cotranscribed and induced by sarcosine. The presence of inducible pathways for metabolism of carbaryl as a nitrogen and carbon source helps in complete and efficient mineralization of carbaryl by strain C5pp, thereby maintaining the biogeochemical cycles.IMPORTANCEThe degradation of xenobiotics plays a significant role in the environment to maintain ecological systems as well as to prevent the imbalance of biogeochemical cycles via carbon-nitrogen cycling. Carbaryl is the most widely used pesticide from the carbamate family.Pseudomonassp. strain C5pp, capable of utilizing carbaryl as a carbon and nitrogen source for its growth, subsequently helps in complete remediation of carbaryl. Thus, it maintains the ecosystem by balancing the biogeochemical cycles. The metabolic versatility and genetic diversity of strain C5pp for the transformation of contaminants like carbaryl and 1-naphthol into less harmful products make it a suitable candidate from the perspective of bioremediation.

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“…Para éste plaguicida también se han reportado algunos microorganismos con capacidad de degradación, entre algunos de los más importantes se pueden mencionar Bacillus sp. (Hamada et al, 2015), A. baumannii (Abou-Shanab et al, 2012), Burkholderia sp. (Castellanos-rozo et al, 2016), Rhizobium sp.…”

Section: Discussionunclassified

Identificación de cepas bacterianas tolerantes a pesticidas aisladas de suelos agrícolas

et al. 2019

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Diversos microorganismos han sido reportados por su eficacia en la biodegradación de contaminantes como plaguicidas gracias a sus características metabólicas. En este estudio, se aislaron e identificaron bacterias de suelos agrícolas las cuales mostraron ser capaces de tolerar pesticidas y utilizarlos como única fuente de carbono y energía. Se evaluaron cinco pesticidas más comúnmente utilizados (sales dimetilaminas, tri-isopropanolaminas y de glifosato, atrazina y carbofuran) a diferentes concentraciones utilizando dos medios de cultivo: TY (Triptona / Extracto de Levadura) y Bushnell Haas (FLUKA). Del medio TY se obtuvieron 13 aislados los cuales toleraron 0.05%, 1, 5 y 10% v/v de la concentración de los pesticidas en el medio de cultivo. Los aislados que toleraron 1% v/v de glifosato y sales dimetilaminas fueron identificados como Bacillus, Microbacterium y Bordetella, mientras que los que toleraron 5 y 10% v/v de sales tri-isopropanolaminas, atrazina y carbofuran en el medio de cultivo corresponden a los géneros Pseudomonas, Enterobacter, Stenotrophomonas y Achromobacter. Por otro lado, al utilizar el medio Bushnell Haas se obtuvieron 9 aislamientos que toleraron concentraciones de 0.05% y 0.5% v/v para el glifosato, 2.5% v/v para sales dimetilaminas, 5% v/v para atrazina y carbofuran. Estos corresponden a los géneros Pseudomonas, Stenotrophomonas, Brevundimonas, Achromobacter y Enterobacter.

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Carbaryl degradation by bacterial isolates from a soil ecosystem of the Gaza Strip

Cited by 30 publications

References 18 publications

Screening of bacterial strains for developing effective pesticide-tolerant plant growth-promoting microbial consortia from rhizosphere soils of vegetable fields of eastern Uttar Pradesh, India

Screening of bacterial strains for developing effective pesticide-tolerant plant growth-promoting microbial consortia from rhizosphere soils of vegetable fields of eastern Uttar Pradesh, India

Carbaryl as a Carbon and Nitrogen Source: an Inducible Methylamine Metabolic Pathway at the Biochemical and Molecular Levels in Pseudomonas sp. Strain C5pp

Identificación de cepas bacterianas tolerantes a pesticidas aisladas de suelos agrícolas

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