Screening of bacterial strains for developing effective pesticide-tolerant plant growth-promoting microbial consortia from rhizosphere soils of vegetable fields of eastern Uttar Pradesh, India

Abstract

The current study was intended to isolate and characterize the plant growth-promoting properties and pesticide (chlorpyripfos) tolerance ability of microbial strains for sustainable vegetable productions. Plant growthpromoting microorganism is a group of microbial consortia to improve crop growth and yield by various direct mechanisms, e.g. nitrogen fixation, phosphate solubilization, production of plant growth hormones, ammonia and siderophore, and indirect mechanisms, e.g. production of antibiotic, hydrogen cyanide to help as biocontrolling agent. Totally, 50 microbes were isolated from soils of vegetable field. Out of 50, 14 strains were selected on the basis of morphological, growth biochemically characters (e.g. gram staining, amylase, cellulase, catalase and citrate test) and plant growth-promoting properties. All strains have ability of ammonia production. Out of 14 strains, IESDV2, IESDV3, IESDV4, IESDV11, IESDV12 and IESD28 were found significant increase in production of IAA, ammonia and phosphate solubilization than others. Strains IESDV3 and IESDV11 showed tolerant at 6 ll/ml concentration of chlorpyrifos which is three-time increase concentration of recommended dose (2 ll/ml). Others strains IESDV12, IESDV13 and IESDV28 were also showed resistance or tolerance at different concentrations of pesticide 2, 4, 6 and 20 ll/ml. These strains also showed plant growth-promoting rhizobacteria activities. Therefore, the main result of this study is to screen and identify that three strains (IESDV3, IESDV11, IESDV12 and IESDV28) can be used as effective pesticide-tolerant plant growth-promoting microbial consortia for vegetable production under sustainable agriculture at eastern Uttar Pradesh.

Kitazin-pea interaction: understanding the fungicide induced nodule alteration, cytotoxicity, oxidative damage and toxicity alleviation byRhizobium leguminosarum

“…For instance, under fungicide free environment, strain RP1 released 80.5 AE 2.5 mg mL À1 of IAA which declined regularly with consistent increase in kitazin concentration but a maximum reduction in IAA synthesis was recorded at 3Â (15.6 AE 0.5 mg IAA mL À1 ). In line with these ndings, Verma et al 42 have also reported similar secretion of IAA by various rhizosphere microbes. The production of IAA even at higher fungicide concentration indeed is a fascinating and encouraging property of soil microbiota, because such fungicide tolerant microbes when applied under fungicide contaminated soil are most likely to continue secreting IAA and making this essential growth enhancing phytohormone available to plants even under fungicide polluted soils.…”

Impact of Plant Growth Promoting Rhizobacteria in Restoring Soil and Crop Attributes

“…Improved nutrient acquisition in T13 (tetra-inoculant) and then T11 and T12 (tri-inoculant) treatments was due to increased nutrient uptake of N from nitrogen-fixing bacteria, uptake of P from phosphate mineral solubilizing bacteriaand uptake of iron from siderophore producing bacteria (Pseudomoas putida and Trichoderma sp.) and suppressing plant diseases (Verma et al, 2016;Jaiswal et al, 2019). It was also reported by Sani et al (2020) that Trichoderma sp.…”

Applications of Natural Violet Pigments from Halophilic Chromobacterium violaceum PDF23 for Textile Dyeing with Antimicrobial and Antioxidant Potentials

“…Enzyme activity such as oxidase, catalase, urease, triple sugar iron agar test, citrate test, indole, MR-VP test [38,39], and motility test were carried out according to method of Litchfield and Gillevet [40].…”