Antibacterial properties of silver nanoparticles synthesized by marine Ochrobactrum sp.

Abstract

Metal nanoparticle synthesis is an interesting area in nanotechnology due to their remarkable optical, magnetic, electrical, catalytic and biomedical properties, but there needs to develop clean, non-toxic and environmental friendly methods for the synthesis and assembly of nanoparticles. Biological agents in the form of microbes have emerged up as efficient candidates for nanoparticle synthesis due to their extreme versatility to synthesize diverse nanoparticles with varying size and shape. In the present study, an eco favorable method for the biosynthesis of silver nanoparticles using marine bacterial isolate has been attempted. Very interestingly, molecular identification proved it as a strain of Ochrobactrum anhtropi. In addition, the isolate was found to have the potential to form silver nanoparticles intracellularly at room temperature within 24 h. The biosynthesized silver nanoparticles were characterized by UV-Vis spectroscopy, transmission electron microscope (TEM) and scanning electron microscope (SEM). The UV-visible spectrum of the aqueous medium containing silver nanoparticles showed a peak at 450 nm corresponding to the plasmon absorbance of silver nanoparticles. The SEM and TEM micrographs revealed that the synthesized silver nanoparticles were spherical in shape with a size range from 38 nm – 85 nm. The silver nanoparticles synthesized by the isolate were also used to explore its antibacterial potential against pathogens like Salmonella Typhi, Salmonella Paratyphi, Vibrio cholerae and Staphylococcus aureus.

Rapid Biosynthesis Method and Characterization of Silver Nanoparticles Using <i>Zizyphus spina christi</i> Leaf Extract and Their Antibacterial Efficacy in Therapeutic Application

“…These results agreed with previous work carried out by Hungund et al [38], Thomas et al [39], and Chitra and Annadurai [40].…”

Green Synthesis of Silver Nanoparticles Using Water Extract from Galls of Rhus Chinensis and Its Antibacterial Activity

“…The poor antibacterial activity is due to the larger particle size of AgNPs [9]. When the particle size of AgNPs is around 10-50 nm, the MIC and MBC are approximately 10 times less than this study results [6][7][8].…”

“…Green synthesis of silver nanoparticles (AgNPs) by using different parts of plant such as leaf [4], stem [5], and root [6], microorganism [7,8], and biopolymers such as starch [9], chitosan [10], lignin [11], latex [12] as a reducing agent, have been reported previously. Besides, studies reported limited synthesis of AgNPs using agricultural waste such as Annono squamosa and mango peel extracts [13,14].…”

“…Polyvinylpyrrolidone, Polyethylene glycol, and surfactants are used as stabilizers to prevent AgNPs aggregation and precipitation [16]. AgNPs exhibit properties that are of interest for applications ranging from antimicrobial [4][5][6][7][8][9][10]12], antioxidant [17], antiproliferative [4,7,11,17] antiplasmodial [18], larvicidal and paste control [19,20], H 2 O 2 sensing [11] along with other applications like medical imaging and drug delivery [21], hyperthermia in cancer therapy [22], and dye reduction [23].…”

Electrospun Polycaprolactone Membrane Incorporated with Biosynthesized Silver Nanoparticles as Effective Wound Dressing Material

“…In our previous study, we have already reported the AgNP-synthesizing properties of a wide range of bacteria [25][26][27]. Among these, Bacillus amyloliquefaciens was found to have an ability to form highly stable AgNPs.…”