Rapid Biosynthesis Method and Characterization of Silver Nanoparticles Using <i>Zizyphus spina christi</i> Leaf Extract and Their Antibacterial Efficacy in Therapeutic Application

Abstract

Antibacterial activity of biosynthesized silver nanoparticles (AgNPs) was significant in therapeutic application of nanotechnology. These researchers studied an ecofriendly and rapid method for the first time to synthesize silver nanoparticles using Zizyphus spina christi L aqueous leaves extract (ZSE), and their antibacterial properties. The extract was found to have the potential to form silver nanoparticles at room temperature within few minutes. The green synthesized silver nanoparticles were characterized using different techniques. The UV-visible spectrum of the solution containing AgNPs showed a peak at 414 nm corresponding to the plasmon absorbance of silver nanoparticles. The transmission electron microscopy (TEM) showed that the formed particles were hexagonal in shape with appreciable Nano size ranging from 21.5 to 59.67 nm. Fourier Transform Infrared Spectroscopy analysis (FTIR) of biosynthesized AgNPs affirmed the role of ZSE as reducing and capping agent of Ag + ions to AgNPs, and X-Ray Diffraction patterns (XRD) showed that they could be indexed as face-centered-cubic structure of silver. Antibacterial activity of AgNPs was determined by well diffusion and micro plate assay methods, showing maximum inhibition zones of 24 mm, 23 mm, 15 mm and 17 mm against Staphylococcus aureus, Acinetobacter sp., Pseudomonas aeruginosa and Escherichia coli respectively.The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) results showed that AgNPs had MIC, MBC of 45, 57 μg/mL, 49, 61 μg/mL, 63, 90 μg/mL and 59, 82 μg/mL against S. aureus, Acinetobacter sp., P. aeruginosa and E. coli respectively. Furthermore, the green synthesized AgNPs were loaded on band-aids and screened for antibacterial activity. The AgNPs loaded on band-aids exhibited strong antibacterial effect against multi drug resistant bacteria. These nanoparticles could be used for treating wounds and preparing wound dressing. Such researches are crucial in the demonstration of therapeutic importance of silver nanoparticles in medical application.

A comparative study of stability, antioxidant, DNA cleavage and antibacterial activities of green and chemically synthesized silver nanoparticles

“…These peaks were due to the organic compounds that were present in the extract. Based on the results phytochemical coating of green synthesized nanoparticles caused some additional peaks at the X-ray spectra in comparison with sharp and unique peaks for chemically synthesized nanoparticles [50]. The antioxidant activity of D. stramonium aqueous extract has been previously reported, and it was very similar to the current study [51].…”

Biologically Synthesized Silver Nanoparticles for Enhancing Tetracycline Activity Against Staphylococcus aureus and Klebsiella pneumoniae

“…Sidr leaves extract reduced the metal ions under all parameters used and the absorbance peak was detected by UV-Vis Spectrophotometer analysis. Similar finding has been reported in previous studies [34][35][36]. Garlic extract at concentrations [20, 25 gm ̸ 100 distilled water], boiling for 5-10 minutes, added at a percentage of 10 mL ̸ 90 mL aqueous silver ions, molar silver ion concentration [1 mM, 2mM] gave yellowish to faint brown color after incubation for 48 hours.…”

Chemical Synthesis of Silver Nanoparticles Using Tri-sodium Citrate, Stability Study and Their Characterization

“…The X-Rays are generated by a cathode ray tube, filtered to produce monochromatic radiation and directed towards the sample. The interaction of the incident rays with the sample produces constructive interference, when conditions satisfy Bragg's law [16]. Synthesized Ag NPs (1 g) were uniformly spread on glass sample holder and placed in scanning chamber.…”