Phytochemical content of plant extracts can be used effectively to reduce the metal ions to nanoparticles in one-step green synthesis process. In this study, six plant extracts were used for the synthesis of silver nanoparticles (AgNPs). Biologically synthesized AgNPs was characterized using UV-Vis Spectrophotometer, Field Emission Scanning Electron Microscope (FE-SEM), X-ray diffraction (XRD), Energy Dispersive X-ray spectroscopy (EDX) and Fourier Transform Infrared (FTIR) spectroscopy. The individual and combined effects of AgNPs and tetracycline against S. aureus and K. pneumoniae were HIGHLIGHTS• Interest in nanoparticles has attracted our attention toward applications in managing bacterial diseases.• Significant differences between the tested plant extracts in supporting AgNPs synthesis.• AgNPs synthesized by ginger showed the highest antibacterial activity.• AgNPs significantly enhanced tetracycline activity against tested bacteria.2Masoud, E A.; et al.