Green Synthesis of Silver Nanoparticles Using Water Extract from Galls of Rhus Chinensis and Its Antibacterial Activity

Abstract

The green synthesis of silver nanoparticles (AgNPs) has been proposed as a simple, eco-friendly and cost effective alternative to chemical and physical methods. The Rhus chinensis plant is one of the well studied medicinal plant and its galls find excellent clinical and therapeutic applications. The present study reports the use of water extract from galls of R. chinensis as a reducing agent and formation of AgNPs from silver nitrate solution by a green synthesis route. The AgNPs formation was observed visually by color change and the absorbance peak at 450 nm was observed by UV-Visible spectrophotometer. The shape, size, and morphology of synthesized AgNPs were monitored by transmission electron microscopy and field-emission scanning electron microscopy. The face centered cubic structure of AgNPs was confirmed by X-ray diffraction pattern and element composition by energy dispersive X-ray analysis. The Fourier transform infrared spectroscopy spectrum revealed that the presence of components acts as a reducing and capping agent. The antibacterial activity was performed using the agar well diffusion method. Minimum inhibitory concentration and minimum bactericidal concentration were determined by broth dilution and spread plate method respectively. Synthesized nanoparticles were spotted as triangular and hexagonal shape and the particle size was around 150 nm.

Cyclic voltammetry, photocatalytic and antimicrobial comparative studies of fabrication Fe3O4 and Fe3O4/PAN nanofibers

“…The zones of inhibition observed against the test pathogens were lower than those obtained in PANF. The antimicrobial activity of the nanoparticles supports the previous observation reported in literature in AgNPs synthesized with R. chinensis galls extract and marine algae (Ecklonia cava) respectively [55,56]. Contrary to the above observations, antibacterial activities of the synthesized nanoparticles against Salmonella Typhimurium and Salmonella Enteritidis were not dependent on concentration of Fe 3 O 4 NPs.…”

Antimicrobial, Antioxidant, and Anticancer Activities of Biosynthesized Silver Nanoparticles Using Marine Algae Ecklonia cava

“…The S. aureus colonies treated with 40 µg of AgNPs exhibited a larger diameter of zone of inhibition ( Figure 8 (Bd)), as compared to those treated with aqueous extract of Ecklonia cava alone ( Figure 8 (Bb)). It was reported that synthesized AgNPs with Rhus chinensis extracts exhibited efficient antimicrobial activity against S. aureus, Staphylococcus saprophyticus , E. coli , and Pseudomonas aeruginosa [ 61 ]. This study used 50 µg to 70 µg of AgNPs to achieve good antibacterial activity against all of the tested bacteria.…”

“…Antimicrobial activity of biosynthesized AgNPs against microorganisms was assessed by an agar well diffusion method as previously described [ 61 ]. Briefly, both Escherichia coli and Staphylococcus aureus were cultured in 3 mL of Luria–Bertani broth.…”

Facile green synthesis of silver nanoparticles using Mangifera indica seed aqueous extract and its antimicrobial, antioxidant and cytotoxic potential (3-in-1 system)

“…The cell wall of Gram-negative bacteria is made up of thin peptidoglycan layer while that of Gram-positive bacteria is made up of thick peptidoglycan layer [39]. The antimicrobial activity also depends on nanoparticle concentration used and the type of bacterial species [40].…”