Integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae

Mobarak-Qamsari, Maryam; Ashayeri-Panah, Mitra; Eftekhar, Freshteh; Feizabadi, Mohammad Mehdi

doi:10.1590/s1517-83822013000300028

Cited by 17 publications

(7 citation statements)

References 22 publications

(37 reference statements)

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“…11,47 Since, only a rare studies about class 3 integron has been described and categorized 10,48,50 Mobarak-Qamsari et al, described 22 (44%) integron class 1, only 3 (6%) isolates of K. pneumoniae carried integron class 2 and none of them contained of integon class 3. 51 Possible critical role of integrons genes in distribution of ESBL-encoding bla TEM gene among ESBL-producing K. pneumoniae clinical isolates may be inferred. This highpoints the increasing complication of antimicrobial agent resistance problems, and the explanations for this condition need further investigation.…”

Section: Discussionmentioning

confidence: 98%

Molecular Identification of Extended-Spectrum β-lactamase and Integron Genes in Klebsiella pneumonia

et al. 2016

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Introduction: Infections caused by Gram negative bacteria, producing extended-spectrum β-lactamase, including Klebsiella pneumoniae are increasing all over the world with high morbidity and mortality. The aim of the present study was determined antimicrobial profile susceptibility and the prevalence of antibiotic resistance genes by multiplex PCR. Methods: In the present study, we obtained one-hundred isolates of K. pneumoniae from different clinical samples. The antibiotic susceptibility testing was done in thirteen antibiotic and, therefore, M-PCRs were conducted using the DNA amplification for detection of ESBLs (blaTEM, blaCTX-M, blaSHV) and int (I, II, III) genes. Results: The results of resistance to amoxicillin/clavulanate, ciprofloxacin, amikacin, trimethoprim-sulfamethoxazole, cefotaxime, ampicillin, aztreonam, imipenem, gentamicin, ceftazidime, Cefepime, ceftriaxone and levofloxacin were obtained 37%, 37%, 93%, 84%, 52%, 87%, 59%, 8%, 24%, 67%, 52%, 43% and 26%, respectively. The frequency of the extended-spectrum β-lactamase K. pneumoniae was obtained 37%. The prevalence of resistance genes of ESBLs in the M-PCR method showed that the blaTEM, blaCTX and blaSHV were 38%, 24% and 19%, respectively, however, only 8 (8%) out of 100 isolates were found to have positive outcomes for the existence of class 1 integrons and there were no detected class 2 or class 3 integrons. Conclusions: Our results recommend the likely co-carriage of some ESBLs genes and antibiotic resistance integrons on the same plasmids harboring the MDR genes. Keywords: fKlebsiella pneumonia, integrons, drug resistance. | PubMed

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Section: Discussionmentioning

confidence: 98%

Molecular Identification of Extended-Spectrum β-lactamase and Integron Genes in Klebsiella pneumonia

et al. 2016

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“…Class III integrons have been reported only in very few studies 14,62,63 . MobarakQamsari et al 64 identified 22 (44%) class I integron-carrying K. pneumoniae isolates, but only three (6%) of the isolates had class II integrons and none contained class III integrons 64 . These findings suggest that class I integron genes may play a critical role in the distribution of β-lactamase-encoding genes among clinical β-lactamase-producing K. pneumoniae isolates.…”

Section: -59mentioning

confidence: 99%

Molecular detection of β-lactamase and integron genes in clinical strains of Klebsiella pneumoniae by multiplex polymerase chain reaction

Sedighi

Halajzadeh

Ramazanzadeh

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction:Infections caused by β-lactamase-producing gram-negative bacteria, such as Klebsiella pneumoniae, are increasing globally with high morbidity and mortality. The aim of the current study was to determine antimicrobial susceptibility patterns and the prevalence of antibiotic resistance genes (β-lactamase and integron genes) using multiplex PCR. Methods: One-hundred K. pneumoniae isolates were collected from different clinical samples. Antibiotic susceptibility testing was performed with thirteen different antibiotics. Multiplex-PCR was used to detect β-lactamase (bla TEM , bla CTX-M, bla SHV, bla VEB , bla PER , bla GES , bla VIM, bla IMP, bla OXA , and bla KPC ) and integron genes (int I, int II, and int III). Results: The highest and lowest rate of resistance was exhibited against amikacin (93%) and imipenem (8%), respectively. The frequency of β-lactamase-positive K. pneumoniae was 37%, and the prevalence of the bla TEM , bla CTX-M, bla SHV, bla VEB , bla PER , bla GES , bla VIM, bla IMP, bla OXA , and bla KPC genes was 38%, 24%, 19%, 12%, 6%, 11%, 33%, 0%, 28%, and 23%, respectively. Of the 100 isolates, eight (8%) were positive for class I integrons; however, class II and III integrons were not detected in any of the strains. Conclusions: These results indicate co-carriage of a number of β-lactamase genes and antibiotic resistance integrons on the same plasmids harboring multi-drug resistance genes. It seems that these properties help to decrease treatment complications due to resistant bacterial infections by rapid detection, infection-control programs and prevention of transmission of drug resistance.

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“…Several reports stated that the presence of integrons in ESBL coding gene plasmid has a high correlation with MDR. This may occur if the ARI and ESBL coding genes are within one integron complex or within one plasmid [11,12].…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, Indonesia

et al. 2019

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Background and Aim: Klebsiella pneumoniae is one of the respiratory disease agents in human and chicken. This bacterium is treated by antibiotic, but this treatment may trigger antibiotic resistance. Resistance gene in K. pneumoniae may be transferred to other bacteria. One of the known resistance genes is extended-spectrum β-lactamase (ESBL). This research aimed to study K. pneumoniae isolated from chicken farms in East Java, Indonesia, by observing the antibiotic resistance pattern and detect the presence of ESBL coding gene within the isolates. Materials and Methods: A total of 11 K. pneumoniae isolates were collected from 141 chicken cloacal swabs from two regencies in East Java. All isolates were identified using the polymerase chain reaction method. Antimicrobial susceptibility was determined by agar dilution method on identified isolates, which then processed for molecular characterization to detect ESBL coding gene within the K. pneumoniae isolates found. Results: The result of antibiotic sensitivity test in 11 isolates showed highest antibiotic resistance level toward ampicillin, amoxicillin, and oxytetracycline (100%, 100%, and 90.9%) and still sensitive to gentamicin. Resistance against colistin, doxycycline, ciprofloxacin, and enrofloxacin is varied by 90.9%, 54.5%, 27.3%, and 18.2%, respectively. All isolates of K. pneumoniae were classified as multidrug resistance (MDR) bacteria. Resistance gene analysis revealed the isolates harbored as blaSHV (9.1%), blaTEM (100%), and blaCTX-M (90.9%). Conclusion: All the bacterial isolates were classified as MDR bacteria and harbored two of the transmissible ESBL genes. The presence of antibiotic resistance genes in bacteria has the potential to spread its resistance properties.

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Integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae

Cited by 17 publications

References 22 publications

Molecular Identification of Extended-Spectrum β-lactamase and Integron Genes in Klebsiella pneumonia

Molecular Identification of Extended-Spectrum β-lactamase and Integron Genes in Klebsiella pneumonia

Molecular detection of β-lactamase and integron genes in clinical strains of Klebsiella pneumoniae by multiplex polymerase chain reaction

Molecular detection of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates of chicken origin from East Java, Indonesia

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