Use of Readycult™ - LMX for enumeration of total coliforms and Escherichia coli in milk

Abstract

Readycult™ -LMX is a rapid method to test for the presence of total coliform bacteria and Escherichia coli in water, giving results in 24h. It is based on reactions of specific microbial enzymes or indicator nutrients of the medium. The goal of this paper was to study the use of Readycult™ -LMX to detect total coliform and E. coli in milk. One hundred twenty five samples of pasteurized and raw milk, collected in Londrina, PR, Brazil, were tested simultaneously by the most probable number (MPN) method using Brilliant Green Bile Lactose Broth (2%) (BRILA) and Readycult™ -LMX. The Readycult™ -LMX test was evaluated for sensitivity, simplicity, and speed of results. There was a significant correlation between the results obtained by the two methods for total coliforms (r: 0.8224) and for E. coli (r: 0.8603). The two methods yielded similar results, but Readycult™ -LMX was easier to use. In addition, results were available as early as 24h.

Isolation of Escherichia coli and Staphylococcus aureusin raw milk from refrigeration tanks: identification and antimicrobial resistance profiles

“…In addition to conventional microbiological analyses, quick analysis methods like Readycult™ -LMX (BELOTI et al, 2002), Rida Count [Coliforms R 1009] (VASALLO; REYES; CAMBAS, 2013), and 3M TM PetrifilmTM Staph (SOUZA et al, 2015) are routinely applied to microbiological milk analyses. Among these methods, 3M TM Petrifilm TM products are recognized and regulated for foods of animal origin by the Brazilian Ministry of Agriculture, Livestock and Supply-MAPA (BRASIL, 2005).…”

Selective colorimetric detection of Gram-negative re-contaminants in pasteurised milk products by a novel application of the BacT/ALERT 3D system

“…They often can be partly automated or integrated in existing laboratory information management systems. Different microbiological parameters have been analysed using novel cultural techniques in pasteurised milk: coliforms (Foschino, Colombo, Crepaldi, & Baldi, 2003;Kang & Gray, 2002;Madden & Gilmour, 1995;Raybaudi, Zea, Curini, & Martínez, 2005), Pseudomonas species (Van Tassell et al, 2012), Escherichia coli and coliforms (Beloti, Barros, Nunes, De Santana, & Nero, 2002;Feldsine et al, 2005), total bacterial counts and coliforms (Ginn, Packard, & Fox, 1986), standard plate counts, psychrotrophic bacterial counts, and coliforms (Senyk, Kozlowski, Noar, Shipe, & Bandler, 1987) and total viable counts, coliforms, and Enterobacteriaceae (Firstenberg-Eden, Foti, McDougal, & Baker, 2002). Furthermore, novel detection techniques and procedures have been presented for the detection of recontaminants in pasteurised milk, such as the determination of microbial activity using a microrespirometer (Ren & Hsieh, 2005), the detection of coliforms by menadione-catalysed luminol chemiluminescence (Kawasaki et al, 2007), the detection of microbial spoilage of milk using Fourier transform infrared spectroscopy and chemometrics (Nicolaou & Goodacre, 2008), the application of a matrix-assisted laser desorption/ionisation timeof-flight mass spectrometry (MALDI-TOF-MS) and multivariate analysis (Nicolaou, Xu, & Goodacre, 2012), and finally the specific detection of total viable coliforms, as well as viable Enterobacteriaceae, by PCR-based techniques (Soejima, Minami, Ayeshima, & Iwatsuki, 2012a;Soejima, Minami, Yaeshima, & Iwatsuki, 2012b).…”