The aim of this study was to investigate the changes in physicochemical characteristics and free amino acid profile occurring in immature vegetable soya bbean during postharvest storage under three temperatures [5, 10 and 20 (control), AE1°C] for 7 days. The results showed that a lower temperature provided an effective control in reducing weight loss, maintained firmness, delayed changes in the pod colour and soluble sugars concentration. Twenty-two free amino acids in immature soya beans also identified by the 1 H NMR spectroscopy were strongly affected by postharvest temperature, and there were time-specific differences in the concentration. Those significant differences in free amino acids concentration among storage conditions were closely associated with aspartate and glutamate degradation. Additionally, after 7-day storage at 5 and 10°C, the soya bean grains accumulated the highest amount of some essential and flavour amino acids, which revealed storage conditions selected should be dictated by the goal.