A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4

Acevedo, Francisca; Pizzul, Leticia; Castillo, María del Pilar; Rubilar, Olga; Lienqueo, María Elena; Díez, M.C.

doi:10.1590/s1516-89132011000600013

Cited by 16 publications

(19 citation statements)

References 55 publications

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“…Production of laccase in the same media, though evident from day 1 itself, reached maximum on day 3, and started to decline subsequently when production of MnP started exponentially. MnSO4, Tween 80, and sodium malonate are inducers for MnP biosynthesis in different WRF (Perez and Jeffries 1992;Acevedo et al 2011;Järvinen et al 2012;Usha et al 2014). Production of laccase did not drastically increase unlike MnP in the media used.…”

Section: Incubation (Day) Incubation (Day)mentioning

confidence: 84%

Screening of wild basidiomycetes and evaluation of the biodegradation potential of dyes and lignin by manganese peroxidases

Rao

Ravichandran

Kandalam

et al. 2019

BioRes

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Manganese peroxidase (MnP), a crucial enzyme in biodegradation of lignin, is synthesized by most white rot fungi. To obtain novel enzymes with superior biodegradation potential, MnP-producing wild isolates were evaluated for their ability to degrade recalcitrant azo dyes, sulfonephthalein dyes, and kraft lignin. Of 30 wild isolates screened, 18 tested positive for lignin modifying enzymes (LMEs). Thirteen of these isolates were positive for both laccase and MnP, whereas four produced only laccase, and one produced lignin peroxidase alone. The isolates were identified as Clitopilus scyphoides MH172162 (AGUM004), Ganoderma rasinaceum MH172163 (AGUM007), and three Schizophyllum species: MH172164, MH172165, and MH172166 (KONA001, AGUM0011, and AGUM021). The Fourier-transform infrared spectroscopy (FTIR) analysis of dye degradation and kraft lignin samples with AGUM004 and AGUM007 revealed biotransformation. The former could not completely degrade Reactive Black 5 and bromocresol green, but it could completely (100%) decolorize bromophenyl blue, bromothymol blue, and Remazol brilliant blue R. The latter efficiently degraded almost all tested dyes. Both degraded kraft lignin. The screened hyper MnP-producing wild AGUM004 and AGUM007 were shown to be potential dye degraders in addition to having lignin degrading abilities.

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Section: Incubation (Day) Incubation (Day)mentioning

confidence: 84%

Screening of wild basidiomycetes and evaluation of the biodegradation potential of dyes and lignin by manganese peroxidases

Rao

Ravichandran

Kandalam

et al. 2019

BioRes

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“…The reaction mixture contained 120 µL of 10 mM DMP, 240 µL of 250 mM sodium tartrate buffer (pH 4), 120 µL of 10 mM MnSO 4 , 120 µL of 4 mM H 2 O 2 and 600 µL of sample. The mixture was incubated at 60 °C for one minute and the absorbance of the mixture was measured at 469 nm ( ε = 10,000 M −1 cm −1 ) [ 20 , 21 ]. Lignin peroxidase activity was determined using the method described by Tien and Kirk [ 22 ] with modifications.…”

Section: Methodsmentioning

confidence: 99%

High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised Trametes hirsuta PW17-41 and FTIR Analysis

Sarp

Suwannasai

2022

Microorganisms

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Coloured wastewater from the textile industry is a very serious global problem. Among 16 different white-rot fungal isolates, Trametes hirsuta PW17-41 revealed high potential for decolourisation of mixed textile dyes (Navy EC-R, Ruby S3B and Super Black G) from real industrial wastewater samples. The efficiency of dye decolourisation was evaluated using the American Dye Manufacturers’ Institute (ADMI) standard methodology. The suitable support for fungal mycelium immobilisation was nylon sponges. The optimal dye decolourisation (95.39%) was achieved by using palm sugar and ammonium nitrate as carbon and nitrogen sources, respectively. The initial pH was 5 and the agitation speed was 100 rpm at 30 °C. The ADMI values of textile dyes decreased from 2475 to 114 within two days, reducing the treatment time from seven days before optimisation. The major mechanism of dye decolourisation was biodegradation, which was confirmed by UV–visible and FTIR spectra. Manganese peroxidase (MnP) (4942 U L−1) was found to be the main enzyme during the decolourisation process at an initial dye concentration of 21,200 ADMI. The results indicated the strong potential of immobilised fungal cells to remove high concentrations of textile dyes from industrial wastewater and their potential ability to produce high MnP and laccase activities that can be used in further application.

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“…The lignocellulose content in the substrate is known to act as an inducer in enzyme production. Inducers are compounds or elements that support reactions in enzymes and support enzyme secretion (Acevedo et al, 2011). Table 6 shows that the longer the fermentation time, the lower the activity of the MnP enzyme produced.…”

Section: Manganese Peroksidase Activitymentioning

confidence: 99%

Lignin Content, Ligninase Enzyme Activity and in vitro Digestability of Sugarcane Shoots using Pleurotus ostreatus and Aspergillus oryzae at Different Fermentation Times

Pazla¹,

Jamarun²,

Warly³

et al. 2021

American Journal of Animal and Veterinary Sciences

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This study aims to obtain the best lignin content, ligninase enzyme activity and in vitro digestibility value of fermented sugarcane shoots using Pleurotus ostreatus and Aspergillus oryzae at different fermentation times. The research process is divided into 2 stages. Stage 1 treatment samples include A1B1 = Sugarcane shoots fermented with Pleurotus ostreatus for 14 days; A1B2 = Sugarcane shoots fermented with Pleurotus ostreatus for 21 days; A1B3 = Sugarcane shoots fermented with Pleurotus ostreatus for 28 days; A2B1 = Sugarcane shoots fermented with Aspergillus oryzae for 14 days; A2B2 = Sugarcane shoots fermented with Aspergillus oryzae for 21 days; A2B3 = Sugarcane shoots fermented with Aspergillus oryzae for 28 days. The design used is a completely randomized design with a factorial pattern. Stage 2 treatment samples cover A = Sugarcane shoots fermented with Pleorotus ostreatus for 21 days; B = Sugarcane shoots fermented with Pleorotus ostreatus for 28 days; C = Sugarcane shoots fermented with Aspergillus oryzae for 21 days; D = Fermented sugarcane shoots with Aspergillus oryzae for 28 days. The design used was a randomized block design. The results showed that there was no interaction between the type of mold and the fermentation time on the lignin content (P>0.05), but there was an interaction with the enzyme activity of Laccase, LiP and MnP (P<0.05). Also, there were significant differences in the digestibility of protein, cellulose, hemicellulose, VFA and NH3 (P<005), however the digestibility of DM, OM, ADF, NDF and rumen fluid pH had no significant difference (P>0.05). It was concluded that sugarcane shoots fermented with Pleurotus ostreatus mold for 28 days got the best results with the value as follow, lignin content (11.55%), CP digestibility (57.90%), cellulose digestibility (50.25%), Hemicellulose digestibility (62.65%), Laccase enzyme activity (2.68 U/mL), LiP enzyme activity (19.44 U/mL), VFA (111.67 mM) and NH3 (10.48 mg/100 mL).

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A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4

Abstract: In this study, different growth conditions of Anthracophyllum discolor

Cited by 16 publications

References 55 publications

Screening of wild basidiomycetes and evaluation of the biodegradation potential of dyes and lignin by manganese peroxidases

Screening of wild basidiomycetes and evaluation of the biodegradation potential of dyes and lignin by manganese peroxidases

High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised Trametes hirsuta PW17-41 and FTIR Analysis

Lignin Content, Ligninase Enzyme Activity and in vitro Digestability of Sugarcane Shoots using Pleurotus ostreatus and Aspergillus oryzae at Different Fermentation Times

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