High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised Trametes hirsuta PW17-41 and FTIR Analysis

Abstract

Coloured wastewater from the textile industry is a very serious global problem. Among 16 different white-rot fungal isolates, Trametes hirsuta PW17-41 revealed high potential for decolourisation of mixed textile dyes (Navy EC-R, Ruby S3B and Super Black G) from real industrial wastewater samples. The efficiency of dye decolourisation was evaluated using the American Dye Manufacturers’ Institute (ADMI) standard methodology. The suitable support for fungal mycelium immobilisation was nylon sponges. The optimal dye decolourisation (95.39%) was achieved by using palm sugar and ammonium nitrate as carbon and nitrogen sources, respectively. The initial pH was 5 and the agitation speed was 100 rpm at 30 °C. The ADMI values of textile dyes decreased from 2475 to 114 within two days, reducing the treatment time from seven days before optimisation. The major mechanism of dye decolourisation was biodegradation, which was confirmed by UV–visible and FTIR spectra. Manganese peroxidase (MnP) (4942 U L−1) was found to be the main enzyme during the decolourisation process at an initial dye concentration of 21,200 ADMI. The results indicated the strong potential of immobilised fungal cells to remove high concentrations of textile dyes from industrial wastewater and their potential ability to produce high MnP and laccase activities that can be used in further application.

Screening of some Brazilian fungi and bacteria able to decolor a textile effluent and evaluation of effluent toxicity on Artemia salina

“…For example, in the work of Noreen et al (2021), who used laccase enzymes from T. versicolor immobilized in a matrix of polyvinyl alcohol (PVA) and alginate for the decoloration of various textile dyes and achieved a decoloration of 92% of the Blue reactive dye and 77.4% of the Acid Black 172. Thampraphaphon et al (2022) tested the ability of the fungus T. hirsuta to decolorize a mixed textile dyes (Navy EC-R, Ruby S3B and Super Black G) in a real sample of textile effluent, thus achieving an optimal decolorization of 95.39% in the presence of ammonium nitrate. The species T. lactinea was shown as a source for biotreatment of textile effluent with its ability to remove indigo-carmine dyes with a percentage of 85.06% (Santana et al, 2021).…”

Exploring the cellulolytic and hemicellulolytic activities of manganese peroxidase for lignocellulose deconstruction

“…Manganese peroxidase (MnP, EC 1.11.1.13), a well-known lignin-degrading peroxidase, can oxidatively depolymerize lignin in an H 2 O 2 -assisted reaction by oxidizing Mn 2+ to Mn 3+ , which is subsequently chelated by organic acids forming a diffusible oxidant to degrade lignin [ 20 , 21 ], aromatic compounds [ 22 , 23 ], pollutants and dyes [ 24 – 26 ]. MnPs are promising biocatalysts for converting lignin-based feedstock into high-value products, such as bioethanol and others, through lignin deconstruction/delignification [ 27 ].…”

Biodegradation of Azo Dye Methyl Red by Pseudomonas aeruginosa: Optimization of Process Conditions

“…Recently, many new approaches for dye biodegradation have been developed which are considered as cost-effective and environmentally friendly. Therefore, biological treatments using microorganisms to degrade synthetic dyes are being investigated as viable and cost-effective alternatives as compared with other treatment approaches [ 24 , 25 ].…”