Genetic divergence in sweet cassava cultivars using morphological agronomic traits and RAPD molecular markers

Rimoldi, Fabrício; Filho, Pedro Soares Vidigal; Kvitschal, Marcus Vinícius; Gonçalves‐Vidigal, Maria Celeste; Prioli, Alberto José; Prioli, Sônia Maria Alves Pinto; Costa, Tiago Ribeiro da

doi:10.1590/s1516-89132010000600025

Cited by 19 publications

(23 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The characterization of plant materials using morphological traits has being used to assess the genetic diversity of cassava (Sambatti et al, 2001;Raghu et al, 2007;Benesi et al, 2010;Asare et al, 2011), to determine the divergence among genotypes (Vieira et al, 2008;Rimoldi et al, 2010), and to verify the correlation among agronomic traits (Vieira et al, 2008). The use of these characters allows rapid identification of germplasm bank accessions and has the advantage of the fact that most traits show a high heritability, without suffering interference from the environment (Fukuda et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

“…Several studies have reported morphological characterization to determine the genetic diversity among cassava genotypes (Sambatti et al, 2001;Benesi et al, 2010;Raghu et al, 2007;Vieira et al, 2008;Rimoldi et al, 2010;Asare et al, 2011). Molecular markers have also being used to estimate genetic diversity parameters for cassava originated from Brazil and other countries, such as: RAPD (random amplified polymorphic DNA) (Lokko et al, 2006;Ferreira et al, 2008;Rimoldi et al, 2010), SSR (simple sequence repeats) (Moyib et al, 2007;Siqueira et al, 2009Siqueira et al, , 2010Alves-Pereira et al, 2011;Montero-Rojas et al, 2011;Oliveira et al, 2012;Costa et al, 2013), and AFPL (amplified fragment length polymorphism) (Benesi et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Molecular markers have also being used to estimate genetic diversity parameters for cassava originated from Brazil and other countries, such as: RAPD (random amplified polymorphic DNA) (Lokko et al, 2006;Ferreira et al, 2008;Rimoldi et al, 2010), SSR (simple sequence repeats) (Moyib et al, 2007;Siqueira et al, 2009Siqueira et al, , 2010Alves-Pereira et al, 2011;Montero-Rojas et al, 2011;Oliveira et al, 2012;Costa et al, 2013), and AFPL (amplified fragment length polymorphism) (Benesi et al, 2010). SSR markers are particularly attractive because they are abundant in plants, showing codominance and high levels of polymorphism (Mba et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Morphological and molecular diversity among cassava genotypes

Mezette

Blumer

Veasey

2013

Pesq. agropec. bras.

View full text Add to dashboard Cite

-The objective of this work was to characterize morphologically and molecularly the genetic diversity of cassava accessions, collected from different regions in Brazil. A descriptive analysis was made for 12 morphological traits in 419 accessions. Data was transformed into binary data for cluster analysis and analysis of molecular variance. A higher proportion of white or cream (71%) root cortex color was found, while flesh colors were predominantly white (49%) and cream (42%). Four accession groups were classified by the cluster analysis, but they were not grouped according to their origin, which indicates that diversity is not structured in space. The variation was greater within regions (95.6%). Sixty genotypes were also evaluated using 14 polymorphic microsatellite markers. Molecular results corroborated the morphological ones, showing the same random distribution of genotypes, with no grouping according to origin. Diversity indices were high for each region, and a greater diversity was found within regions, with: a mean number of alleles per locus of 3.530; observed and expected heterozygosity of 0.499 and 0.642, respectively; and Shannon index of 1.03. The absence of spatial structure among cassava genotypes according to their origins shows the anthropic influence in the distribution and movement of germplasm, both within and among regions.Index terms: Manihot esculenta, genetic diversity, germplasm, microsatellites, morphological traits. Diversidade morfológica e molecular entre genótipos de mandiocaResumo -O objetivo deste trabalho foi caracterizar, morfológica e molecularmente, a diversidade genética de acessos de mandioca de diferentes regiões do Brasil. Realizou-se a análise descritiva de 12 características morfológicas em 419 acessos. Os dados foram transformados em dados binários para análise de agrupamento e análise de variância molecular. Observou-se maior proporção (71%) de cor branca ou creme do córtex da raiz, enquanto as cores da polpa foram predominantemente branca (49%) e creme (42%). Quatro grupos de acessos foram classificados pela análise de agrupamento, mas não foram agrupados de acordo com sua origem, o que indica que a diversidade não está estruturada no espaço. A maior parte da variação ocorreu dentro das populações (95,6%). Sessenta genótipos também foram avaliados com o uso de 14 marcadores microssatélites polimórficos. Os resultados moleculares corroboraram os morfológicos e mostraram a mesma distribuição aleatória dos genótipos, com ausência de agrupamento quanto ao local de origem. Os índices de diversidade foram elevados para cada região e a maior parte da diversidade foi observada dentro de regiões, com: número médio de alelos por loco de 3,530; heterozigosidade média observada de 0,499 e esperada de 0,642; e índice de Shannon de 1,03. A ausência de estrutura espacial entre os genótipos de mandioca de acordo com suas origens mostra a influência antrópica na distribuição e movimentação de germoplasma, tanto entre como dentro de regiões.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphological and molecular diversity among cassava genotypes

Mezette

Blumer

Veasey

2013

Pesq. agropec. bras.

View full text Add to dashboard Cite

show abstract

“…BGB 48 was included in many of the most divergent combinations evaluated suggesting that this genotype could provide an acceptable heterotic effect when employed as a parent in hybridization programs (Table 2) combinations that aggregate the maximum number of desirable agronomical traits (Rimoldi et al, 2010). Indeed, diallelic crossing is one of the tools that can be employed for the best genotypes (Lima and Cardoso, 2000).…”

Section: Discussionmentioning

confidence: 99%

Multivariate analysis of ‘bacuri’ reproductive and vegetative morphology

Souza

Damasceno‐Silva

et al. 2016

Com. Sci.

View full text Add to dashboard Cite

The objective of this study was to characterize sixteen genotypes of P. insignis available in the Embrapa Meio-Norte germplasm collection (Teresina, Piauí, Brazil) with respect to 33 morphological traits relating to leaves, flowers, branches, fruits and seeds. Phenotypic variance among genotypes was estimated using the Mahalanobis distance technique and the unweighted pair group method with arithmetic mean analysis (UPGMA). The method of Singh (1981) was used to determine which of the traits contributed most to diversity within genotypes. The occurrence of phenotypic variability among P. insignis genotypes indicated that it would be possible to achieve positive gains with selection. The most distant genotypes were BGB 16 and BGB 48, while crosses between genotype BGB 48 and genotypes BGB 32 and BGB 56 offers the greatest potential as parental types for this fruit tree breeding programs . The flesh content, ovary and fruit length , ratio between fruit length and diameter were the characters that most contributed to diversity among the studied genotypes. Keywords: phenotypic diversity, Platonia insignis, Mahalanobis distanceAnálise multivariada da morfologia reprodutiva e vegetativa do bacurizeiro Resumo O objetivo do trabalho foi caracterizar dezesseis genótipos de bacurizeiro disponível na Coleção de Germoplasma da Embrapa Meio-Norte (Teresina, Piauí, Brasil) com base em 33 caracteres relacionados à folha, flor, ramos, fruto e semente. A divergência fenotípica entre os genótipos foi estimada pela distância de Mahalanobis e a análise de agrupamento dos genótipos por meio do método hierárquico da ligação média não ponderada (UPGMA). O método de Singh foi utilizado na determinação de quais caracteres mais contribuem para a diversidade dos genótipos. Observou-se diversidade fenotípica significativa entre os genótipos estudados, indicando que é possível obter ganhos genéticos importantes por meio da seleção. Os genótipos BGB 16 e BGB 48 são os mais divergentes. Entretanto, cruzamentos entre o genótipo 48 e os genótipos BGB 32 e BGB 56 oferecem potencial como parentais em programas de melhoramento da frutífera. O teor de polpa, comprimento do fruto, comprimento do ovário e relação comprimento / diâmetro do fruto são os caracteres que mais contribuem para a diversidade entre os genótipos estudados.Palavras-chave: diversidade fenotípica, Distância de Mahalanobis, Platonia insignis

show abstract

“…Em estudos com mandioca diversos trabalhos apresentam a caracterização morfológica como forma de determinação da diversidade genética entre genótipos coletados nas mais diferentes regiões do país (PERONI et al, 1999;SAMBATTI et al, 2001;VIEIRA et al, 2008;KVITSCHAL et al, 2009;ALBUQUERQUE et al, 2009;RIMOLDI et al, 2010;SIVIERO;SCHOTT, 2011).…”

Section: Caracteres Morfológicos E Agronômicosunclassified

Diversidade agronômica, bioquímica e molecular de acessos de mandioca (Manihot esculenta Crantz) coletados em diferentes regiões do Brasil

Mezette¹

View full text Add to dashboard Cite

Agronomic, biochemical and molecular diversity among cassava accessions (Manihot esculenta Crantz) collected in different regions in BrazilA relevant species as food source for the world population, especially for the underdeveloped and emerging countries, is cassava (Manihot esculenta Crantz). Primarily, cassava is a provider of energy from starch accumulated in its roots, but the presence of carotenoids with pro-vitamin A activity is also important. In this context, the objective of this study was to characterize the agronomic, biochemical and molecular diversity of cassava accessions selected for the color of their roots, collected in the Amazon, Central-West, Southeast and South regions in Brazil. The characterization was performed from 81 genotypes (33 from the Amazon region, 24 from Central-West, 18 from Southeast, four from the South and two commercial varieties) using 16 agronomic traits, five biochemical characters and 17 microsatellite loci. For biochemical and agronomic traits analyses of variance, Tukey test and Mahalanobis distances were obtained considering the region of origin of the genotypes. For microsatellite markers, indices of genetic diversity were estimated, such as number of alleles per locus (A), percentage of polymorphic loci (P), observed (H o ) and expected (H e ) heterozygosities, as well as the structuring of diversity between and within groups (H S , H T , D ST and G ST ). Also, a cluster analysis was obtained using the Neighbor-Joining method and Nei´s genetic distance, as well as a Bayesian analysis. Moreover, the distances obtained for all characters were correlated using the Mantel test. Results indicate high variability for all characters evaluated. For most of agronomic traits there was significant variation between and within regions. All biochemical characters showed highly significant variation between and within regions. Taking into account the agronomic characters, there was a tendency for the Amazon genotypes to be grouped together, where the characteristics total plant height, height of the first branch, relationship between these characteristics and harvest index were those that mostly determined the genotypes distribution. For the biochemical characters, the Amazon genotypes were also grouped due to the high concentration of cyanogenic compounds and total carotenoids. Molecular markers indicated high genetic variability (A = 3.58; P = 100%; H o = 0.535; H e = 0.642), while most of total diversity (H T = 0.651) was found within regions (H S = 0.643). In the cluster analysis a tendency was observed towards a structured genetic variability, with the grouping of most genotypes collected in the Amazon region. The Bayesian analysis separated the genotypes in two groups, with one of the groups including most of the Amazon genotypes, in accordance with the cluster analysis. The correlations between the distances obtained for all characters were not significant. Considering the genotypes content of cyanogenic compounds and the distance between them from the microsate...

show abstract

Genetic divergence in sweet cassava cultivars using morphological agronomic traits and RAPD molecular markers

Abstract: ABSTRACT

Cited by 19 publications

References 18 publications

Morphological and molecular diversity among cassava genotypes

Morphological and molecular diversity among cassava genotypes

Multivariate analysis of ‘bacuri’ reproductive and vegetative morphology

Diversidade agronômica, bioquímica e molecular de acessos de mandioca (Manihot esculenta Crantz) coletados em diferentes regiões do Brasil

Contact Info

Product

Resources

About