Overview of parameters influencing biomass and bioreactor performance used for extracellular ligninase production from Phanerochaete chrysosporium

Effluents from the textile industry have a negative environmental impact due to their high load of dyes and hard-to-remove compounds: additives, detergents, and surfactants; these must be treated before effluents can be discharged into water. White-rot fungi show great potential for the bioremediation of water and soil matrices contaminated with recalcitrant pollutants (these are generally toxic). In this work, we designed a 5 L fixed bed reactor and evaluated its performance on the degradation of pollutants in effluents from the textile industry in continuous-operation mode under non-sterile conditions, using ligninolytic fungus Bjerkandera sp. (anamorphic state R1). This setup was based on a previous design of a 0.25 L fixed-bed model bioreactor. The system was designed by taking into account the geometric and hydrodynamic similarities of both setups. In continuous-mode color-removal assays, the bioreactor was operated at a 36 h Hydraulic retention time (HRT), a 1 L/min air flux at 33°C, and a dye concentration of 75 g/L (sulfur black 1) and 6.5 g/L (indigo Vat blue 1). 69% of the dye was removed, and changes in the chemical structures of the dyes confirmed the ligninolytic activity of the microorganism as the main dye removal mechanism.. RESUMEN: Los efluentes provenientes de industrias textiles generan impactos ambientales negativos, debido a altas cargas de colorantes y compuestos de difícil remoción como aditivos, detergentes y surfactantes, los cuales deben ser tratados antes de ser descargados a cuerpos de agua. Los hongos ligninolíticos han mostrado gran potencial para procesos de biorremediación de aguas y suelos contaminados con compuestos recalcitrantes y generalmente tóxicos. Este trabajo, se enfoca en el diseño y evaluación del desempeño de un reactor de 5L de lecho fijo para la degradación de efluentes de la industria textil en condiciones no estériles y operación continua, usando el hongo ligninolítico Bjerkandera sp. en su estado anamorfo R1. Dicha tecnología se desarrolló tomando como base para realizar el diseño un biorreactor modelo de lecho de fijo de 0,25 L. El sistema de 5L se diseñó teniendo en cuenta la similitud geométrica e hidrodinámica. En los ensayos de decoloración en continuo el reactor se operó a un tiempo de retención hidráulica (TRH) de 36 h, aireación de 1 L/min y 33°C, además de una carga de colorante de 75g/L para el Negro sulfuroso y 6,5g/L para índigo Vatblue; se alcanzó una decoloración del 69% y se identificaron cambios en las estructuras químicas de los colorantes presentes en el agua residual después del tratamiento, mostrando la actividad ligninolítica del microorganismo como el principal mecanismo de remoción de color.

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“…The value of axial dispersion can vary along the reactor bed [24]. ratio L/d must stay above 10 for tubular reactors.…”

Section: Variable Hydrodynamic Influence Reactor Length (L)mentioning

confidence: 99%

Evaluating the scale-up of a reactor for the treatment of textile effluents using Bjerkandera sp

Gaviria

Osorio-Echavarría

Gómez-Vanegas

2018

Rev. Fac. Ing. Univ. Antioquia

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“…The samples were collected in sterile plastic bags and stored in a refrigerator (4˚C) prior to use. The WSW was analyzed for its compositions or constituents, while ZD was soaked in warm water, sieved and incubated at 37˚C (24 h) prior to use [6], [20], [21].…”

Section: A Sample Collection and Preparationmentioning

confidence: 99%

“…Phanerochaete chrysosporium BKMF 1767 (ATCC 24725) culture was used for Ligninase production according to the methods described by Ntwampe et al [20]. Lignin peroxidase activity (1 μmol veratryl alcohol oxidized to veratraldehyde per minute) was measured.…”

Section: E Production Of Ligninasesmentioning

confidence: 99%

“…This could be due to influential factors such as the optimum C: N ratio alteration and insufficient exposure to dissolved oxygen [26]. Bioreactor operational conditions play an important role in enzyme activity [20]. AHP had a higher impact on the pretreated samples, attributed to a higher delignification efficacy of the WSW as opposed to the untreated samples.…”

Section: E Enzyme Cocktail Hydrolysismentioning

confidence: 99%

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Enhanced Pretreatment of Winery Solid Waste for High Reducing Sugar Yield

Okudoh¹,

Okeleye²,

Da-Barca³

et al. 2018

ASETH-18,ACABES-18 &Amp; EBHSSS-18 Nov. 19-20 2018 Cape Town (South Africa)

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An increasing quantity of Winery solid waste (WSW) generated annually is of environmental concern. This study was aimed to evaluate the pretreatment efficacy of winery waste for biogas production. Samples comprising mainly of grape pomace were collected from a winery in Stellenbosch, South Africa and subsequently pretreated using chemical (Alkaline hydrogen peroxide, AHP) and biological (cellulases and ligninases) methods. To assess their activity, ligninases and cellulases were further used to hydrolyze the pretreated samples both singly and in combination (cocktail). The results showed an increase in reducible sugar (23%) with the combined enzymes (2.06 mg/mL) compared to the individual enzymes (1.68 mg/mL). The AHP-pretreated biomass also gave higher (1.900 mg/mL) yields than non-AHP pretreated (0.102 mg/mL), indicating a significant effect on delignification and de-crystallinization of cellulose in the biomass. This optimization process had significant impact on the valorization of winery solid waste.

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“…However, only 1,2-dioxygenase (115.7 U/l) and LiP (98.7 U/l) were present in high amounts in the presence of DDT (Table 1). Ntwampe et al (35) reported that the performance of the biomass to produce the required enzymes in liquid cultures was dependent on the presence of readily metabolisable substrates such as glucose. Catalysis of the decomposition of the recalcitrant aromatic compounds in white-rot fungi is affected by extracellular enzymes.…”

Section: Degradation Of Ddt By T Versicolor U97mentioning

confidence: 99%

Determination of co-metabolism for 1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane (DDT) degradation with enzymes from Trametes versicolor U97

Sari

Tachibana

Itoh

2012

Journal of Bioscience and Bioengineering

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Overview of parameters influencing biomass and bioreactor performance used for extracellular ligninase production from Phanerochaete chrysosporium

Abstract: ABSTRACT

Cited by 8 publications

References 74 publications

Evaluating the scale-up of a reactor for the treatment of textile effluents using Bjerkandera sp

Evaluating the scale-up of a reactor for the treatment of textile effluents using Bjerkandera sp

Enhanced Pretreatment of Winery Solid Waste for High Reducing Sugar Yield

Determination of co-metabolism for 1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane (DDT) degradation with enzymes from Trametes versicolor U97

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