Seminal analysis, cryogenic preservation, and fertility in matrinxã fish, Brycon cephalus (Günther, 1869)

Ninhaus‐Silveira, Alexandre; Foresti, Fausto; Veríssimo‐Silveira, Rosicleire; Senhorini, José Augusto

doi:10.1590/s1516-89132006000500015

Cited by 36 publications

(26 citation statements)

References 21 publications

(13 reference statements)

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“…The existing studies using ACP for the cryopreservation of fish semen demonstrate its potential (Viveiros et al ; Viveiros et al ); however, the results remain inconclusive and more research is necessary to understand its role. For the standard solution consisting of DMSO, glucose, and egg yolk, the results obtained for dourado are similar to those observed for other South American fish, such as yamu, Brycon siebenthalae (Cruz‐Casallas et al ), matrinxã, Brycon cephalus (Ninhaus‐Silveira et al ), and cachama blanca, Piaractus brachypomus (Ramirez‐Merlano et al ). However, for other South American fish species, such as B .…”

Section: Discussionsupporting

confidence: 76%

Use of ACP‐104 at Different Dilutions for Sperm Cryopreservation of Dourado, Salminus brasiliensis

Zanandrea¹,

Weingartner²,

Zaniboni‐Filho³

2014

J World Aquaculture Soc

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The dourado, Salminus brasiliensis, is an important fish in South American river basins. The fish‐farming potential and concerns for its conservation in watersheds has stimulated studies with this species. This study compared the effect of different dilutions of two cryoprotectant solutions on the quality of cryopreserved dourado sperm (duration of motility/motility rate) post‐thawing using three different activating solutions. The cryoprotectant solution ACP‐104 was compared with the cryoprotectant solution most commonly used for dourado, which consists of a mixture of dimethyl sulfoxide, glucose, egg yolk, and distilled water. The semen was mixed following dilutions of semen : cryoprotectant solution: 1:3, 1:9, 1:15, 1:21, and 1:27. The cryopreserved samples were activated using distilled water, 0.45% NaCl or 1% NaHCO3. The results showed differences in thawed semen using the different activating solutions and cryoprotectant solutions. The glucose‐based cryoprotectant not only resulted in sperm motility equal to or better than that produced by ACP‐104, but the quality was not affected by the dilution. However, the sperm quality improved with increasing dilutions for ACP‐104. Under the conditions tested, the standard cryoprotectant solution is recommended for the cryopreservation of dourado semen because it requires a lower solution volume and, therefore, reduced storage space for the same volume of semen.

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Section: Discussionsupporting

confidence: 76%

Use of ACP‐104 at Different Dilutions for Sperm Cryopreservation of Dourado, Salminus brasiliensis

Zanandrea¹,

Weingartner²,

Zaniboni‐Filho³

2014

J World Aquaculture Soc

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“…O volume de sêmen observado no dourado (Tabela 1), assim como em outras espécies de peixes brasileiras, apresentou diferenças interespecíficas (Andrade-Talmelli et al, 2001;Ninhaus-Silveira et al, 2006;Bombardelli et al, 2006a), no entanto, essas variações podem ocorrer de acordo com o método de colheita, uma vez que a extrusão não garante a liberação total do sêmen presente nas gônadas ).…”

Section: Resultsunclassified

Dose inseminante para fertilização artificial de ovócitos de dourado

et al. 2009

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RESUMO -Objetivou-se determinar a dose inseminante adequada para uso na fertilização artificial de ovócitos de dourado (Salminus brasiliensis). Os ovócitos foram distribuídos em delineamento inteiramente casualizado, e fertilizados com uma das relações espermatozoides/ovócito 6,0×10 3 ; 6,0×10 4 ; 6,0×10 5 ; 6,0×10 6 ou 3,0×10 7 , cada uma com quatro repetições.Considerou-se unidade experimental uma incubadora de volume útil de 2,5 L, contendo 2,0 mL de ovócitos não-hidratados.As taxas de fertilização foram mensuradas 8 horas após o início da fertilização. Com intuito de verificar possíveis efeitos da diluição seminal na movimentação dos espermatozoides, realizou-se a mensuração do tempo de duração da motilidade espermática dos espermatozoides de dourado, ativados por meio de diferentes relações de diluição: 6,8×10 -5 ; 6,8×10 -4 ; 6,8×10 -3 ; 6,8×10 -2 ; 3,4×10 -1 e 1,0 mL de sêmen por mL de água. O tempo de duração da motilidade foi avaliado em delineamento inteiramente casualizado composto de seis tratamentos e três repetições. As taxas de fertilização apresentaram relação quadrática com o número de espermatozoides por ovócito. As relações de diluição do sêmen tiveram efeito inversamente proporcional sobre a duração da motilidade espermática. A relação que proporcionou melhores taxas de fertilização artificial Insemination dose for artificial fertilization of dourado oocytesABSTRACT -The objective of the present study was to determine the proper insemination dose of dourado (Salminus brasiliensis) oocytes. The oocytes were placed in a randomized complete design and fertilized with one of the spermatozoa.oocytes -1 ratio, 6.0×10 3 , 6.0×10 4 , 6.0×10 5 , 6.0×10 6 , 3.0×10 7 SPZ:OOC, each one with four replications. An experimental unit was considered to be an incubator with a 2.5L useful volume containing 2.0 mL non-hydrated oocytes. The fertilization rates were measured eight hours after the start of fertilization. In order to ascertain the possible effects of seminal dilution on the spermatozoa motility, the duration time of the spermatic motility of the dourado spermatozoa was measured when activated by different dilution ratios 6.8×10 -5 ; 6.8×10 -4 ; 6.8×10 -3 ; 6.8×10 -2 ; 3.4×10 -1 and 1.0 mL semen.mL water -1 . The motility duration time was assessed in a randomized complete design, with six treatments and three repetitions. The fertilization rates showed a quadratic relationship with the number of spermatozoids per oocyte. The semen dilution ratios had an inversely proportional affect on the spermatic motility time. The spermatozoa:oocytes ratio that provided the best artificial fertilization rates of dourado (Salminus brasiliensis) oocytes was 30,722 spermatoids per oocyte.

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“…In the past few years, some studies on fish sperm cryopreservation of species of the subfamily Bryconinae, such as matrinxã or yamú Brycon amazonicus (Cruz‐Casallas, Medina‐Robles & Velasco‐Santamaria 2006; Ninhaus‐Silveira, Veríssimo‐Silveira, Senhorini, Alexandre & Chaguri 2006; Velasco‐Santamaria, Medina‐Robles & Cruz‐Casallas 2006), matrinxã Brycon cephalus (Ninhaus‐Silveira, Foresti, Veríssimo‐Silveira & Senhorini 2006), B. orbignyanus (Maria, Viveiros, Freitas & Oliveira 2006; Maria, Viveiros, Orfão, Oliveira & Moraes 2006), matrinchã Brycon orthotaenia (Melo & Godinho 2006) and B. nattereri (Oliveira, Viveiros, Maria, Freitas & Isaú 2007) were published showing effective protocols. In B. insignis , only one PhD thesis (Shimoda 2004) and no published article was found after searching in the web (Google, PubMed, Scopus and Web of Science).…”

Section: Introductionmentioning

confidence: 99%

Sperm cryopreservation of tiete tetra Brycon insignis (Characiformes): effects of cryoprotectants, extenders, thawing temperatures and activating agents on motility features

Viveiros

Amaral

Orfão

et al. 2010

Aquaculture Research

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The aim of this study was to test the effects of cryoprotectants [dimethyl sulphoxide (DMSO) and methylglycol], extenders (0.9% NaCl, 5% glucose, Beltsville Thawing Solution™ and Merck III™), thawing temperatures (30 and 60 °C) and activating agents (0.29% NaCl and 1% NaHCO3) on the cryopreservation process of tiete tetra Brycon insignis sperm. Sperm was loaded in 0.5 mL straws, frozen in nitrogen vapour at −170 °C and stored in liquid nitrogen. Post‐thaw sperm quality was evaluated in terms of subjective motility rate, quality motility score (0=no movement; 5=rapidly swimming spermatozoa), duration of motility and vitality (eosin–nigrosin staining). Post‐thaw sperm motility rate was greater in methylglycol (76–88%), compared with DMSO (23–59%). In general, the highest quality motility scores were observed when sperm was thawed at 30 °C and triggered in 1% NaHCO3 (3.5–4.3). Duration of motility was longer when triggered in 1% NaHCO3 (95–120 s) compared with 0.29% NaCl (69–107 s). Sperm vitality was not affected by any of the parameters tested and varied from 51% to 69% intact sperm. Brycon insignis sperm frozen in methylglycol combined with any of the extenders tested and using the methods described above yields motility above 57% and that should last long enough to fertilize oocytes.

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Seminal analysis, cryogenic preservation, and fertility in matrinxã fish, Brycon cephalus (Günther, 1869)

Abstract: Aiming to improve fish reproduction techniques, the characterization and cryopreservation of semen of

Cited by 36 publications

References 21 publications

Use of ACP‐104 at Different Dilutions for Sperm Cryopreservation of Dourado, Salminus brasiliensis

Use of ACP‐104 at Different Dilutions for Sperm Cryopreservation of Dourado, Salminus brasiliensis

Dose inseminante para fertilização artificial de ovócitos de dourado

Sperm cryopreservation of tiete tetra Brycon insignis (Characiformes): effects of cryoprotectants, extenders, thawing temperatures and activating agents on motility features

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