Varietal identification of coffee seeds by RAPD technique

Crochemore, Maria Lúcia; Nunes, Liliane Moreira; Andrade, Giselly Aparecida; Molinari, Hugo Bruno Correa; Vasconcellos, Maria Elizabeth da Costa

doi:10.1590/s1516-89132004000100002

Cited by 8 publications

(7 citation statements)

References 4 publications

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“…These primers were selected for their high PIC values ranging from 0.82 for primer OPG10 to 0.94 for primer OPG13. The cultivar identification using RAPD markers is well-documented in studies of molecular characterization (Bianchi et al 2003, Crochemore et al 2004. Fingerprinting based on this marker type was used for identification and characterization of potato cultivars in North America (Sosinski and Douches 1996), Australia (Ford and Taylor 1997) and India (Chakrabarti et al 1998).…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers

Rocha

Paiva

Carvalho

et al. 2010

Crop Breed. Appl. Biotechnol.

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Section: Resultsmentioning

confidence: 99%

Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers

Rocha

Paiva

Carvalho

et al. 2010

Crop Breed. Appl. Biotechnol.

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“…Differences between the template DNA concentration of 2 individuals DNA samples result in the loss or gain of some bands (Bardakci, 1996). The cultivars identification using RAPD markers is well- (Bianchi et al, 2003;Crochemore et al, 2004). Fingerprinting based on this marker type was used for identification and characterization of potato cultivars in North America (Sosinski and Douches, 1996), Australia (Ford and Taylor, 1997) and India (Chakrabarti et al, 1998).…”

Section: Rapd Based Genetic Relationshipsmentioning

confidence: 99%

Molecular Diversity Analysis of Two in vitro and Irradiated Potato Varieties Expressed by Random Amplified Polymorphic DNA

et al. 2018

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Potato buds cvs. ҅ Valor' and 'Spunta' were cultured in vitro on MS solid medium with 0.2 mg -1 BAP. The resulting plantlets were irradiated with gamma radiation doses 10, 20, 30, 40 and 50 Gy. Irradiated segments were transferred onto fresh MS with BAP and plantlets survival percentage was calculated after eight weeks. Gamma radiation caused the death of 3.8% to 81% in cv. ҅ Spunta' and 2.9% to 83.9% in cv. ҅ Valor'. Microtubers produced from irradiated plantlets were decreased with increasing gamma radiation doses, with notable changes in shape, size and numbers. The proline contents in irradiated plantlets were steady increase with gamma radiation doses. The genomic DNA of the two cultivars and ten radiation treatments was amplified with 10 RAPD primers that generated 53 polymorphic bands. The highest number of genetic identity was 0.9672 showed between irradiated plantlets with 20 and 30 Gy in cv. ҅ Valor'. However, the highest genetic distance was 0.3995 observed between irradiated plantlets with dose 20 Gy in cv. ҅ Valor' and 30 Gy in cv. ҅ Spunta'. The dendrogram generated by cluster analysis distinguished the irradiated plantlets genetically.

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“…Differences between the template DNA concentration of 2 individuals DNA samples result in the loss or gain of some bands (Bardakci, 1996). The cultivars identification using RAPD markers is welldocumented in studies of molecular characterization (Bianchi et al, 2003, Crochemore et al, 2004. Fingerprinting based on this marker type was used for identification and characterization of potato cultivars in North America (Sosinski and Douches 1996), Australia (Ford and Taylor 1997) and India (Chakrabarti et al, 1998).…”

Section: Rapd Based Genetic Relationshipsmentioning

confidence: 99%

Molecular Diversity Analysis of Two Irradiated Potato Varieties in vitro Revealed by Random Amplified Polymorphic DNA

El-Fiki

Adam

Rashad

et al. 2018

Journal of Scientific Research in Science

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Potato buds cvs. Spunta and Valor were cultured in vitro on MS solid medium supplemented with 0.2 mg-1 BAP. The resulting plantlets were irradiated with gamma radiation doses 10, 20, 30 and 40Gy. Irradiated single node pieces were transferred onto fresh MS with BAP. Plantlets survival percentage calculated after eight weeks, gamma radiation caused decrease in the survival percentage of micropropagated buds in both Spunta and Valor cultivars ranging from 91.4 % to 28.5% in cv. Spunta and 93.3% to 30.47% in cv. Valor, comparing with the values of the two non-irradiated cultivares 95.2% and 96.2% respectively. Microtubers produced from irradiated plantlets were decreased with increasing gamma radiation doses with changes in size and numbers. The estimated proline content in irradiated plantlets was increased with increasing gamma radiation dose. The genomic DNA of non-irradiated (control) in two cultivars and eight radiation treatments was amplified with 10 RAPD primers that generated 54 polymorphic bands. The highest number of genetic similarity was 0.9672 showed between irradiated plantlets with dose 20 and 30Gy in cv. Valor. However, the highest genetic distance was 0.3995 observed between irradiated plantlets with dose 20Gy in cv. Valor and 30Gy in cv. Spunta. The dendrogram generated by cluster analysis distinguished the irradiated plantlets genetically.

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Varietal identification of coffee seeds by RAPD technique

Abstract: ABSTRACT

Cited by 8 publications

References 4 publications

Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers

Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers

Molecular Diversity Analysis of Two in vitro and Irradiated Potato Varieties Expressed by Random Amplified Polymorphic DNA

Molecular Diversity Analysis of Two Irradiated Potato Varieties in vitro Revealed by Random Amplified Polymorphic DNA

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