Aerobic stability of sugar cane silages with a novel strain of Lactobacillus sp. isolated from sugar cane

Abstract

The objective of this research was to evaluate the effect of the addition of two Lactobacillus strains on the aerobic stability of the sugar cane (Saccharum spp.) silages. The experiment was carried out in a completely randomized design with three replicates in split-plot in time scheme, so that the treatments (silages without inoculant and with novel strain or commercial inoculants) were randomized on plot and in times of aerobic evaluation of the silages (zero, two, four, and six days after the opening of the silos) as subplots. The loss of stability of silages was continuous during the time of aerobic exposure. Inoculants influenced the changes in populations of lactic acid bacteria and yeasts and in the ammoniacal nitrogen, acetic acid and ethanol concentrations. The dry matter, crude protein, soluble carbohydrates contents and pH values showed similar changes in all three silages studied. A linear increase in crude protein content and pH values and decrease in the soluble carbohydrates content were observed. The dry matter contents increased to a maximum of 335 g/kg on fresh matter at four days, followed by reduction after six days. The results showed that the use of inoculants is recommended because it promoted higher production of acetic and propionic acids, reducing the population of yeast and, therefore, improving the aerobic stability of silages

Aerobic stability of sugar‐cane silage inoculated with tropical strains of lactic acid bacteria

“…Decreases in the concentrations of ethanol and acetic acid from 2 days of aerobic exposure of sugar‐cane were observed by Ávila et al . (). The complete disappearance of ethanol after the aerobic exposure of silage is caused by volatilization or by the use of this compound by the acetic bacteria (Spoelstra et al ., ), and because the acetic acid concentration was not increased during the evaluation, we could assume that the reduction in the concentration of ethanol was due to volatilization.…”

“…An increase in pH after silo opening has been observed in previous research (McDonald et al ., ; Ávila et al ., ; Wilkinson and Davies, ). The pH remained stable longer in silages treated with facultative heterofermentative L. plantarum strains than in silages treated with obligatory heterofermentative L. brevis and L. hilgardii strains.…”

Silagem de milho ou de cana-de-açúcar com Lactobacillus buchneri exclusivamente ou em associação com L. plantarum

“…O uso de aditivos à base de bactérias heteroláticas tem sido eficiente para controlar a população de leveduras, o que reduz a fermentação alcoólica na massa ensilada e melhora a estabilidade aeróbia, tanto em silagens de milho (Nishino et al, 2004;Tabacco et al, 2011), quanto de cana-de-açúcar (Siqueira et al, 2007;Mendes et al, 2008;Ávila et al, 2012). Silagens de cana-de-açúcar adicionadas de L. buchneri têm mostrado redução de 50% na produção de etanol (1,9 vs. 4,1% da MS), 17% na perda de gases, 56% na perda total de MS e aumento de 64% na estabilidade aeróbia, em relação às silagens sem adição de lactobacilos (Pedroso et al, 2005;Siqueira et al, 2007;Mendes et al, 2008).…”

Caracterização microbiológica, parâmetros fermentativos e estabilidade aeróbia em silagens de forragens tropicais com aditivos microbianos

“…Embora a fermentação heterolática seja menos eficiente quando comparada à homolática, a melhor estabilidade aeróbia da massa ensilada representa grande vantagem na utilização de aditivos contendo cepas de BAL heteroláticas (KUNG; RANJIT, 2001;NISHINO et al, 2004;MENDES et al, 2008;KRISTENSEN et al, 2010;TABACCO et al, 2011;ÁVILA et al, 2012 …”

“…O uso de inoculantes a base de bactérias heteroláticas têm sido eficiente em controlar a população de leveduras, reduzindo a fermentação alcoólica na massa ensilada, e melhorando a estabilidade aeróbia tanto em silagens de milho (RANJIT; TAYLOR; KUNG, 2002;NISHINO et al, 2004;KRISTENSEN et al, 2010;TABACCO et al, 2011), como de cana-de-açúcar (SIQUEIRA et al, 2007;ÁVILA et al, 2008;MENDES et al, 2008;MAEDA et al, 2011;ÁVILA et al, 2012). Silagens de cana-deaçúcar aditivadas com L. buchneri têm mostrado redução de 50% na produção de etanol (1,9 vs. 4,1% da MS), 17% na perda de gases, 56% na perda total de MS e aumento de 64% na estabilidade aeróbia em relação às silagens não aditivadas (PEDROSO et al, 2002(PEDROSO et al, , 2005SIQUEIRA et al, 2007;MENDES et al, 2008).…”