In vitro bioactivity and cytotoxicity of chemically treated glass fibers

Abstract

Samples of a commercial glass fiber FM® (Fiber Max) were used to test the efficacy of a chemical sol-gel surface treatment to enhance their bioactivity. After treatment with tetraethoxysilane (TEOS), individual fiber samples were soaked into a simulated body fluid (SBF) solution, from which they were removed at intervals of 5 and 10 days. Micrographs obtained by scanning electron microscopy (SEM) analysis of samples chemically treated with TEOS revealed the formation of a hydroxyapatite (HA) coating layer after 5 days into SBF solution. Fourier transform infrared spectroscopic (FTIR) analyses confirmed that the coating layer has P-O vibration bands characteristic of HA. The in vitro cytotoxicity was evaluated using a direct contact test, minimum essential medium elution test (ISO 10993-5) and MTT assay. Fibers immersed in SBF and their extracts exhibited lower cytotoxicity than the controls not subjected to immersion, suggesting that SBF treatment improves the biocompatibility of the fiber

Colloidal stability–slip casting behavior relationship in slurry of mullite synthesized by the USP method

“…minerals) as well as organic compounds [29,30] revealed the sample to have mullite-like molecular groups. As in the case of silicate minerals, the two peaks appeared around at 3400 and 1600 cm À1 may be attributed to the OH stretching vibrations and the deformation vibration of adsorbed water, respectively [31]. The other peak located at 3750 cm À1 may be attributed to the OH stretching vibration arising from the Si-OH groups [32].…”

Influence of morphology on in vitro compatibility of bioactive glasses

“…In this way, the studied materials differ from each other only by morphological aspects, and the deleterious effect which is attributed to fiber morphology can be evaluated without concern about the influence of the preparation route and compositional effects. Because in a previous study it was verified that a pre-treatment of implantable materials in simulated body fluid (SBF) solution could reduce implant rejection [8,9], this study also investigated if the cell viability and cytotoxicity of these bioglasses can be improved by immersion into SBF. Primary culture osteoblasts were used to study cell viability and alkaline phosphatase production.…”

Simulated body fluid and water absorption effects on poly(methyl methacrylate)/hydroxyapatite denture base composites

“…The presence of polar and hydrophilic functional groups in acrylic resins may increase their polarity and facilitate uptake of bound water to form primary and secondary hydration shells around the polar groups via hydrogen bonding [13]. Simulated Body Fluid (SBF) test, a method that is well recognized to characterize the in vitro bioactivity of ceramic materials, consists in their immersion in an aqueous SBF solution which simulates the properties of human plasma for certain period and verifies the formation of the hydroxyapatite (HA) layer on the surface of the samples [14]. The quantity of water molecules and SBF absorbed by the resin matrix of dental resin based composite and the rate of water sorption have been identified as being diffusion controlled.…”