Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells

Abstract

Agaricus blazei Murrill, popularly known as the sun mushroom, is a native mushroom in SP, Brazil, that has been widely used in the treatment of cancer and many other pathologies in different parts of the world. A water-soluble protein-polysaccharide complex (1 ® 6)b-D-glucan has been isolated from its fruiting body that showed immune-modulation activity. From organic extracts, linoleic acid has been isolated and determined to be the main substance with antimutagenic activity. Using both the micronucleus (MN) and comet (single cell microgel electrophoresis) assays, this study determined the genotoxic and antigenotoxic potential of A. blazei (AB) obtained from commercial sources or the following strains: a) strains AB 97/29 (young and sporulated phases); b) a mixture taken from AB 96/07, AB 96/09 and AB 97/11 strains; and c) commercial mushrooms from Londrina, PR and Piedade, SP, designated as AB PR and AB SP, respectively. The extracts from these mushrooms were isolated in chloroform:methanol (3:1) and used in vitro at three different concentrations. V79 cells (Chinese hamster lung cells) were exposed to the extracts under pre-, simultaneous and post-treatment conditions, combined with methyl methanesulfonate (MMS). Under the circumstances of this study, these organic extracts did not show any genotoxic or mutagenic effects, but did protect cells against the induction of micronuclei by MMS.

Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells

“…This is in agreement with the reports by Radaković et al [29] and Guterrez et al [10, 12]. Namely, the exposure of cells to the mushroom in our experiment even leads to a reduction of the baseline level of DNA damage.…”

Evaluation of Agaricus blazei in vivo for antigenotoxic, anticarcinogenic, phagocytic and immunomodulatory activities

“…In light of the results obtained, it appears that supplementation with A. blazei did not show genotoxic activity, and this finding allows us to infer that supplementation with this mushroom, based on this protocol, does not produce DNA damage that leads to the development of cancer. This finding is supported by the studies of Menoli et al (2001), Delmanto et al (2001), Oliveira et al (2002), Bellini et al (2003), Luiz et al (2003), Guterrez et al (2004), Machado et al (2005) and Guterres et al (2005), who reported that the administration of A. blazei, in different forms of extract and concentrations, did not produce genotoxic and/or mutagenic activity.…”

Evaluation of antigenotoxic effects of carotenoids from green algae Chlorococcum humicola using human lymphocytes

“…For this reason, these substances in natural foods are extremely important not only due to their nutritional value but also as prophylactic agents against diseases such as cancer [12] .…”