Evaluation of antigenotoxic effects of carotenoids from green algae Chlorococcum humicola using human lymphocytes

Bhagavathy, S.; Sumathi, P.

doi:10.1016/s2221-1691(11)60203-7

Cited by 22 publications

(12 citation statements)

References 28 publications

(38 reference statements)

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“…The produced metabolites may damage DNA by forming adducts, but also through the induction of ROS that, besides DNA, may also damage proteins and lipids. These molecular alterations can be the subjacent origin of the presently observed increase in the number of SCE and CA, result which is in line with the genotoxic potential of BaP previously demonstrated in in vitro and in vivo short-term tests (Alvarez-González et al, 2011;Bhagavathy and Sumathi, 2012;Sehgal et al, 2012). In the case of BC, our results showed no capacity of the chemical to induce SCE and CA in mouse, a finding which is concordant with previous studies using bacterial tests, and the analysis of micronuclei in vitro and in vivo (Di Sotto et al, 2008Molina-Jasso et al, 2009).…”

Section: Discussionsupporting

confidence: 83%

Antigenotoxic capacity of beta-caryophyllene in mouse, and evaluation of its antioxidant and GST induction activities

2014

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-The present report was designed to determine the antigenotoxic capacity of beta-caryophyllene (BC) on the damage induced by benzo(a)pyrene (BaP) in mouse. We found no genotoxic potential of BC, and a significant inhibitory effect on the number of sister-chromatid exchanges (SCE) and chromosomal aberrations induced by BaP. The three tested doses of the agent (20, 200, and 2,000 mg/kg) produced a dose-dependent decrease of the two evaluated cytogenetic parameters. In comparison with the effect induced by BaP, the best inhibitory effect (about 80%) was obtained with the high tested dose of BC considering the two evaluated parameters. Other aim of the study was to explore whether in this effect participated the BC antioxidant capacity and/or its effect as inducer of GST activity. We found a dose-dependent decrease induced by BC in regard to both the oxidation of lipids and proteins produced by BaP. In the case of GST, when BC was administered alone we found a mean increase of 64% of the enzyme activity, respect to the control level, and when BC was administered in mice treated with BaP the increase obtained with the high dose of BC reached 27%. Therefore, our data established no in vivo genotoxicity by BC, and a significant antigenotoxic potential of the compound, which may be related with its capacity to block the molecular oxidation and to stimulate the GST activity.

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Section: Discussionsupporting

confidence: 83%

Antigenotoxic capacity of beta-caryophyllene in mouse, and evaluation of its antioxidant and GST induction activities

2014

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“…Indeed, it has been shown that polyunsaturated fatty acids (PUFAs) played a protective role in B[a]P-induced carcinogenesis by significantly reducing DNA adduct levels [16]. It has also been reported that the green alga Chlorococcum humiloca, rich in carotenoids, inhibits the genotoxicity of B[a]P in human lymphocytes [17]. Thus, these studies support the hypothesis that microalgae can be used as a protective agent.…”

Section: Introductionmentioning

confidence: 60%

Protective Action of Ostreococcus Tauri and Phaeodactylum Tricornutum Extracts towards Benzo[a]Pyrene-Induced Cytotoxicity in Endothelial Cells

Goff

Delbrut²,

Quinton³

et al. 2019

Marine Drugs

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Marine microalgae are known to be a source of bioactive molecules of interest to human health, such as n-3 polyunsaturated fatty acids (n-3 PUFAs) and carotenoids. The fact that some of these natural compounds are known to exhibit anti-inflammatory, antioxidant, anti-proliferative, and apoptosis-inducing effects, demonstrates their potential use in preventing cancers and cardiovascular diseases (CVDs). Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon (PAH), is an ubiquitous environmental pollutant known to contribute to the development or aggravation of human diseases, such as cancer, CVDs, and immune dysfunction. Most of these deleterious effects are related to the activation of the polycyclic aromatic hydrocarbon receptor (AhR). In this context, two ethanolic microalgal extracts with concentrations of 0.1 to 5 µg/mL are tested, Ostreoccoccus tauri (OT) and Phaeodactylum tricornutum (PT), in order to evaluate and compare their potential effects towards B[a]P-induced toxicity in endothelial HMEC-1 cells. Our results indicate that the OT extract can influence the toxicity of B[a]P. Indeed, apoptosis and the production of extracellular vesicles were decreased, likely through the reduction of the expression of CYP1A1, a B[a]P bioactivation enzyme. Furthermore, the B[a]P-induced expression of the inflammatory cytokines IL-8 and IL1-β was reduced. The PT extract only inhibited the expression of the B[a]P-induced cytokine IL-8 expression. The OT extract therefore seems to be a good candidate for counteracting the B[a]P toxicity.

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“…A higher level of ROS is connected with some promutagenic lesions in DNA that can represent an early carcinogenic event. Algae extract rich in fatty acids, lutein, β-carotene and α-carotene was proved to protect against environmental genotoxic factors that cause ROS production [147]. The micronuclei and comet assay revealed that pelargonidin and chlorogenic acid have protective effect against genotoxic agents [148].…”

Section: Genomic Stability and Non-genotoxic Effects Of Dietary Phytomentioning

confidence: 99%

Nutrigenomics in cancer: Revisiting the effects of natural compounds

Braicu

Mehterov

Vladimirov

et al. 2017

Seminars in Cancer Biology

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Nutrigenomics effects have an important role in the manipulation of dietary components for human benefit, particularly in cancer prevention or treatment. The impact of dietary components, including phytochemicals, is largely studied by nutrigenomics, looking at the gene expression and molecular mechanisms interacting with bioactive compounds and nutrients, based on new 'omics' technologies. The high number of preclinical studies proves the relevant role of nutrigenomics in cancer management. By deciphering the network of nutrient-gene connections associated with cancer, relevant data will be transposed as therapeutic interventions for this devastating pathology and for fulfilling the concept of personalized nutrition. All these are presented under the nutrigenomics canopy for a better comprehension of the relation between ingested phytochemicals and chemoprevention or chemotherapy. The profits from the nutrigenomics progress, with a particular focus on the coding and noncoding genes related to the exposure of natural compounds need to be validated. A precise attention receives the evaluation of the role of natural compounds in tandem with conventional therapy using genomic approaches, with emphasis on the capacity to inhibit drug resistance mechanisms. All these relevant nutrigenomics aspects are summarized in the present review paper. It is concluded that further nutrigenomics studies are required to improve our understanding related to the complex mechanisms of action of the natural compounds and for their appropriate application as gears in cancer therapy.

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Evaluation of antigenotoxic effects of carotenoids from green algae Chlorococcum humicola using human lymphocytes

Cited by 22 publications

References 28 publications

Antigenotoxic capacity of beta-caryophyllene in mouse, and evaluation of its antioxidant and GST induction activities

Antigenotoxic capacity of beta-caryophyllene in mouse, and evaluation of its antioxidant and GST induction activities

Protective Action of Ostreococcus Tauri and Phaeodactylum Tricornutum Extracts towards Benzo[a]Pyrene-Induced Cytotoxicity in Endothelial Cells

Nutrigenomics in cancer: Revisiting the effects of natural compounds

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