Fluorescent amplified fragment length polymorphism (fAFLP) analyses and genetic diversity in Litopenaeus vannamei (Penaeidae)

Abstract

The Pacific white shrimp, Litopenaeus vannamei (Penaeidae), represents about 95% of all Brazilian shrimp production. The Brazilian L. vannamei foundation broodstock was made up of specimens collected from different American Pacific sites, but little information was collected on the genetic structure of the broodstock. We used the fluorescence amplified fragment length polymorphism (fAFLP) method to study the genetic diversity of L. vannamei broodstock lines 03CMF1 and 03CBF1 originally produced by breeder-shrimps imported mainly from Panama and Ecuador, although wild individuals from other localities may also have been used in producing these two lines. Our results showed a total of 93 polymorphic bands ranging from 50 to 500 bp, the mean Nei's genetic diversity calculated for the total sample was 13.4% and identity and genetic distance analyses indicated high genetic homogeneity within and between both the broodstock lineages studied which suggests that they had similar genetic structure. These results may represent an important tool for the appropriate management of L. vannamei broodstocks.

Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae)

“…Printed in Brazil www.sbg.org.br 2004) and Litopenaeus setiferus (Ball and Chapman, 2003) but, however, little is known on the genetics of the Brazilian L. vannamei broodstocks Galetti Jr 2002, 2005;De Francisco and Galetti Jr, 2005;Gonçalves et al, 2005). In the work described in the present paper we used microsatellite analysis to investigate the genetic variation in three consecutive generations (F 5 , F 6 and F 7 ) of a Brazilian closed breeder L. vannamei lineage.…”

Diversidade genética de Litopenaeus vannamei cultivado na Bahia

“…A Bahia é o terceiro maior produtor de camarão em cativeiro do Brasil, e a região de Canavieiras, no Sul do Estado, possui um número significativo de fazendas de cultivo e é a que mais se destaca nessa atividade. O Litopenaeus vannamei, conhecido como camarão-branco-dopacífico, foi introduzido no Brasil no início dos anos 80 e é, atualmente, a principal espécie de camarão marinho cultivada no país (Gonçalves et al, 2005).…”

Inheritance of AFLP markers and genetic linkage analysis in two full-sib families of the marine shrimp <i>Litopenaeus vannamei</i> (Crustacea, Decapoda)

“…The fAFLP reactions were performed using the Applied Biosystems AFLP Plant Mapping Protocol Kit [18], originally derived from Vos et al [13]. The genomic DNA (50 ng/µL) was digested with EcoRI and MseI before ligation of adaptors.…”

“…Conventional AFLP is particularly useful for genomic mapping and genetic fingerprinting and may be used to calculate genetic distances between individual members of a population [13,14]. AFLP has successfully been used in the identification of loci linked to important quantitative traits in penaeid marine shrimps [8].…”

“…AFLP has successfully been used in the identification of loci linked to important quantitative traits in penaeid marine shrimps [8]. They do not require a previous knowledge of the genome of the studied organism, and produce a large number of polymorphic loci with highly reproducible band patterns, and high pairing specificity between the used primers and the complementary adaptor nucleotides, which correspond to the original positions in the genome of the species and, consequently, may be explored as marks in the physical and/or genetic map [13].…”