Diversidade genética de Litopenaeus vannamei cultivado na Bahia

Magalhães, Marcelo; Martinez, Romari Alejandra; Gaiotto, Fernanda Amato

doi:10.1590/s0100-204x2007000800009

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…This is particularly important in a situation when no free access to wild populations is available. In Brazil, the reduction in genetic variability and significant differentiation among specific lines has already been reported (Freitas & Galetti Junior, 2002;Francisco & Galetti Junior, 2005;Gonçalves et al, 2005;Magalhães et al, 2007). In the present study, the observed genetic differentiation among hatcheries was reflected by FST values ranging from 0.006 to 0.175 (Table 4).…”

Section: Resultssupporting

confidence: 77%

Genetic variability of marine shrimp in the Brazilian industry

Maggioni

Coimbra

Costa

et al. 2013

Pesq. agropec. bras.

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-The objective of this work was to estimate the genetic variability level and distribution in Brazilian broodstocks of marine shrimp (Litopenaeus vannamei). Nine of the country's largest hatcheries were evaluated using codominant and highly polymorphic microsatellite markers. The results obtained from genotyping of ten microsatellite loci are indicative of genetic variability that is compatible with that found in wild populations of L. vannamei in Mexico and Central America. A possible explanation is the highly diversified and relatively recent origin of the available broodstocks. Bayesian analysis detected a signal for five founding populations. The distribution of genetic distances partially reflects geographical location, and this information will be useful for the creation of new broodstocks. Therefore, L. vannamei genetic variability among nine of the largest national hatcheries can be considered high.Index terms: Litopenaeus vannamei, aquaculture, microsatellite, shrimp farming. Variabilidade genética do camarão marinho na indústria brasileiraResumo -O objetivo deste trabalho foi estimar o nível e a distribuição da variabilidade genética em reprodutores brasileiros do camarão marinho (Litopenaeus vannamei). Nove dos maiores laboratórios de maturação do país foram analisados por meio de marcadores microssatélites codominantes e altamente polimórficos. Os resultados obtidos a partir da genotipagem de dez locos de microssatélites são indicativos de variabilidade genética compatível com aquela encontrada em populações naturais de L. vannamei no México e na América Central. Uma possível explicação é a origem altamente diversificada e relativamente recente dos plantéis disponíveis. A análise bayesiana detectou sinal correspondente a cinco populações originais. A distribuição das distâncias genéticas reflete parcialmente a localização geográfica, e essa informação será útil na formação de novos plantéis. Portanto, a variabilidade genética de L. vannamei entre nove dos maiores laboratórios de maturação nacionais pode ser considerada alta.Termos para indexação: Litopenaeus vannamei, aquacultura, microssatélite, carcinicultura.

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Section: Resultssupporting

confidence: 77%

Genetic variability of marine shrimp in the Brazilian industry

Maggioni

Coimbra

Costa

et al. 2013

Pesq. agropec. bras.

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“…Random Amplified Polymorphic DNA analysis is also being used in population genetics studies like genetic diversity, divergence within and among populations based on assumption of Hardyweinberg equilibrium (Brown, 2003). It detects the genetic variations in the genome of an organism in terms of sequence variation at the priming regions (Magalhães et al, 2007). Similarities in banding profiles among strains (i.e.…”

Section: Randomly Amplified Polymorphic Dna (Rapd)mentioning

confidence: 99%

Application of Molecular Markers in Forensic Botany

Ishfaq

Saleem

2016

Bull. Biol. All. Sci. Res.

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Molecular markers based on DNA sequence have become a remarkable tool in the Forensic Sciences for the identification of culprits. Now a day’s majority of criminal cases are being solved based on DNA evidence from different biological materials like blood, boon, semen, nails with skin piece, hair with hair follicle, spores and any plant part etc. available at the scene of crime. Presently, DNA evidence from plants have also played an important role in solving forensic cases and DNA from any plant part found at the site of incidence can be used to locate the murderers, kidnapers, victims or in arresting drug traffickers. All molecular markers are not useful in Forensic Botany, only some molecular markers are used for plant DNA evidence which includes DNA barcoding, RAPD (Random Amplified Polymorphic DNA), RFLP (Restriction Fragment Length Polymorphism), AFLP (Amplified Fragment Length Polymorphism), SNP (Single Nucleotide Polymorphism) and Microsatellites, but the most widely used molecular marker for plant evident is SSR (Simple Sequence Repeats) due to its high reproducibility with great discrimination power and error free results from small piece of evidence.

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“…Despite enormous advances in molecular techniques that have been used to obtain genetic data, random amplified polymorphic DNA (RAPD) remains a useful approach (Williams et al, 1990) for several reasons (Lacerda et al, 2002;Magalhães et al, 2007;Brahmane et al, 2008;Dutra et al, 2008;Soares et al, 2008). First, it can be used to evaluate populations for which no specific marker has been developed, allowing then a quick screening of genetic variability.…”

Section: Introductionmentioning

confidence: 99%

Optimizing reproducibility evaluation for random amplified polymorphic DNA markers

Ramos¹,

Mp²,

Diniz‐Filho³

et al. 2008

Genet. Mol. Res.

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ABSTRACT. The random amplified polymorphic DNA (RAPD) technique is often criticized because it usually shows low levels of repeatability; thus it can generate spurious bands. These problems can be partially overcome by rigid laboratory protocols and by performing repeatability tests. However, because it is expensive and time-consuming to obtain genetic data twice for all individuals, a few randomly chosen individuals are usually selected for a priori repeatability analysis, introducing a potential bias in genetic parameter estimates. We developed a procedure to optimize repeatability analysis based on RAPD data, which was applied to evaluate genetic variability in three local populations of Tibochina papyrus, an endemic Cerrado plant found in elevated rocky fields in Brazil. We used a simulated annealing procedure to select the smallest number of individuals that contain all bands and repeated the analyses only Optimizing reproducibility evaluation for RAPD markers for those bands that were reproduced in these individuals. We compared genetic parameter estimates using HICKORY and POPGENE softwares on an unreduced data set and on data sets in which we eliminated bands based on repeatability of individuals selected by simulated annealing and based on three randomly selected individuals. Genetic parameter estimates were very similar when we used the optimization procedure to reduce the number of bands analyzed, but as expected, selecting only three individuals to evaluate the repeatability of bands produced very different estimates. We conclude that the problems of repeatability attributed to RAPD markers could be due to bias in the selection of loci and primers and not necessarily to the RAPD technique per se.

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Diversidade genética de Litopenaeus vannamei cultivado na Bahia

Cited by 4 publications

References 18 publications

Genetic variability of marine shrimp in the Brazilian industry

Genetic variability of marine shrimp in the Brazilian industry

Application of Molecular Markers in Forensic Botany

Optimizing reproducibility evaluation for random amplified polymorphic DNA markers

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