Inheritance of AFLP markers and genetic linkage analysis in two full-sib families of the marine shrimp <i>Litopenaeus vannamei</i> (Crustacea, Decapoda)

Abstract

The cultivation of the marine species of shrimp Litopenaeus vannamei has emerged as one of the segments that best develop in the Brazilian aquaculture, representing the agribusiness that grew the most in recent years. Regarding the economic importance of the farming of this species in Brazil, further studies on genetic improvement were conducted. A F2 segregating population consisting of 192 samples for each G1 and G2 families, from crossing inbred lines was used for the studies performed in the present work. The genetic linkage analysis was based on polymorphic markers derived from nine AFLP (Amplified Fragment Length Polymorphism) primers. Fourteen genetic linkage groups including 103 segregating polymorphic markers were constructed covering 350 cM for G1 and four genetic linkage groups including 59 markers were constructed covering 300 cM for G2. Simple marker analyses were performed among individuals evaluated phenotypically, finding markers linked to genes that may be potentially important and useful to assess characteristics of economic importance for traits related to weight and the disease IMN (idiopathic muscle necrosis). The statistical model including markers explained major proportion of phenotypic characteristic weight in relation to disease incidence IMN.

The State of “Omics” Research for Farmed Penaeids: Advances in Research and Impediments to Industry Utilization

“…Linkage maps are now available that include between 3,959 and 9,298 markers and cover all 44 chromosomes of the penaeid genome (Baranski et al, 2014 ; Yu et al, 2015 ; Lu et al, 2016b ; Jones et al, 2017a ). When compared to earlier maps (Wilson et al, 2002 ; Li et al, 2003 ; Pérez et al, 2004 ; Wuthisuthimethavee et al, 2005 ; Li Z. et al, 2006 ; Maneeruttanarungroj et al, 2006 ; Alcivar-Warren et al, 2007 ; Zhang et al, 2007 ; Staelens et al, 2008 ; Andriantahina et al, 2013 ; Gonçalves et al, 2014 ) that contained 27 to 451 markers, distributed across between 8 and 51 linkage groups, the increase in density of polymorphic markers recently achieved has dramatically increased the applicability of these resources (i.e., assisting genomic assembly, examining the genetic architecture of traits, and undertaking comparative mapping).…”

“…Linkage maps are highly versatile genomic resources which provide a wealth of genomic information and facilitate the examination of the underlying genetic architecture of commercially and biologically important traits. As such, the development of linkage maps for penaeids (Table 2 ) has recently seen significant research effort with a number of maps now available for P. monodon (Wilson et al, 2002 ; Wuthisuthimethavee et al, 2005 ; Staelens et al, 2008 ; Baranski et al, 2014 ), L. vannamei (Pérez et al, 2004 ; Alcivar-Warren et al, 2007 ; Zhang et al, 2007 ; Du et al, 2010 ; Andriantahina et al, 2013 ; Gonçalves et al, 2014 ; Yu et al, 2015 ; Jones et al, 2017a ), M. japonicus (Li et al, 2003 ; Lu et al, 2016b ), and F. chinensis (Li Z. et al, 2006 ; Sun et al, 2008 ; Tian et al, 2008 ; Liu et al, 2010 ; Wang et al, 2012 ; Zhang et al, 2013 ). Linkage map construction involves the genetic analysis of family groups (both parents and progeny), to allow the identification of the recombination pattern of polymorphic markers, and in turn, the calculation of a relative genomic position for each marker.…”

Boxer crabs induce asexual reproduction of their associated sea anemones by splitting and intraspecific theft

“…AFLP is an efficient, fast and low cost DNA fingerprinting method ( Bensch & Åkesson, 2005 ; Meudt & Clarke, 2007 ), particularly when studying organisms with limited prior knowledge of their genome ( Uthicke & Conand, 2005 ). In addition, there is an increasing interest in the use of AFLP on marine invertebrates ( Uthicke & Conand, 2005 ; Peng et al, 2012 ; Goncalves et al, 2014 ), particularly cnidarians ( Amar et al, 2008 ; Reitzel et al, 2008 ; Chomsky et al, 2009 ; Douek, Amar & Rinkevich, 2011 ; Brazeau, Lesser & Slattery, 2013 ). If the crabs in nature behave like those observed in the laboratory, namely, frequent “splitting” and theft of sea anemones, we would expect to see high levels of genetic identity between each sea anemone pair.…”

Identification of genomic regions and candidate genes associated with growth ofEriocheir Sinensisby QTL mapping and marker annotation

“…Growth is a complex trait, which is polygenic or determined by the contributions from numerous genes in a quantitative manner (Kroymann & Mitchell‐Olds ). Over the last decade, a number of studies have been performed to map quantitative trait loci (QTLs) for growth traits based on linkage analysis in decapoda species with commercial value, including Portunus trituberculatus (Liu, Li, Liu, Zhao, Gao & Du ), Fenneropenaeus chinensis (Wang, Tian, Kong, Li, Liu & Yang ), Litopenaeus vannamei (Andriantahina, Liu & Huang ; Gonçalves, de Almeida Regitano, Cruz, Salgado, de Freitas, Rocha, Teixeira & Galetti‐Junior ), Marsupenaeus japonicas (Lyons, Dierens, Tan, Preston & Li ) and Penaeus monodon (Robinson, Gopikrishna, Baranski, Katneni, Shekhar, Shanmugakarthik, Jothivel, Gopal, Ravichandran & Gitterle ). Most of these analyses are usually conducted by using a few hundred markers and the average marker intervals are high, which have relatively low resolution for QTL mapping.…”