Development of microsatellite markers for the genetic analysis of Magnaporthe grisea

Brondani, C.; Brondani, R. P. V.; Garrido, L. da R.; Ferreira, Márcio Elias

doi:10.1590/s1415-47572000000400009

Cited by 31 publications

(37 citation statements)

References 16 publications

(21 reference statements)

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“…A presença de alelos nulos em vários organismos é citada na literatura (BRONDANI et al, 2000;CRUZ et al, 2009;SANTOS et al, 2010) e, embora isso possa dificultar as análises, esses alelos não devem ser descartados, pois refletem uma nova fonte de variabilidade genética e podem ser usados na discriminação de indivíduos. No lócus MB02, ocorreu um alelo extra em alguns dos isolados, alelo este que não era esperado, uma vez que o FOC é um organismo haplóide (BENTLEY et al, 1998).…”

Section: Methodsunclassified

Diversidade genética por marcadores moleculares em Fusarium oxysporum f. sp. cubense no Estado de Santa Catarina

et al. 2010

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O mal-do-panamá, causado por Fusarium oxysporum f. sp. cubense (FOC), é um dos principais problemas fitossanitários da bananicultura. O uso de cultivares resistentes é o método preferencialmente recomendado para o seu controle, sendo a avaliação da diversidade genética do patógeno necessária no desenvolvimento de estratégias de manejo da doença a longo prazo. Assim, este trabalho teve como objetivo estudar a variabilidade genética de isolados de FOC por marcadores moleculares RAPD e SSR. Foram avaliados 64 isolados coletados em regiões produtoras do estado de Santa Catarina, sendo que 100% deles foram patogênicos à bananeira da cv. 'Enxerto'. As análises de conglomerados com esses marcadores revelaram variabilidade entre os isolados amostrados. As técnicas moleculares aplicadas foram eficientes em separar os isolados em três grupos distintos. Os membros de cada grupo, em cada uma das técnicas, em geral, foram coincidentes e três dos isolados (CO16, JS23 e JS26) apresentaram-se mais distantes geneticamente nos dendrogramas de similaridade.

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Section: Methodsunclassified

Diversidade genética por marcadores moleculares em Fusarium oxysporum f. sp. cubense no Estado de Santa Catarina

et al. 2010

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“…This overview equally gives a typical foundation of genetic markers that could be helpful for population genetics and to recognize the different species and isolates of organisms and fungi. The SSRs act as codominant Mendelian markers that are more frequent and reproducible than dominant markers and are extensively used to find out the genetic makeup of population (Taylor et al, 1999;Brondani et al, 2000). In different genomes the frequencies of various microsatellites have been assessed by hybridization or database searches.…”

Section: Introductionmentioning

confidence: 99%

Evaluating Short Sequence Repeats (SSRs) Markers in Sordaria fimicola by High Resolution Melt Analysis

Arif

Lee

Saleem

2017

IJAB

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Sordaria fimicola is found worldwide but little is known about its population structure in any region. To bridge this knowledge gap, we attempted to develop molecular markers to study the genetic variations and population structure in the natural strains of S. fimicola. Based on the whole genome sequence of S. macrospora we targeted sixteen genomic regions that contain short sequence repeats (SSRs). Primers were then designed to amplify the homologous regions in S. fimicola. We used high resolution melt (HRM) analyses and amplicon sequencing to analyse the genetic diversity of the wild strains of S. fimicola collected from the opposing slopes (i.e., South facing slope and North facing slope) of the Evolution Canyon (EC), representing contrasting environments. We found twelve regions that were homologous to that of S. macrospora containing (CAT)7, (TTG)7, (T)14, (GAA)7, (CGT)6, (AAGCAC)6, (GT)5, (GGC)6 (ACA)8, (CTG)7 (CT)6 and (CAGGGG)5 were first time reported in S. fimicola. During this genotyping of SSRs we found variations in SSRs motif number and enrichment in strains isolated from stressed and harsh environment than strains from natural and benign environment in Evolution Canyon. The findings favour the hypothesis that genetic variations are present in strains of stressed environment.

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“…Besides, these markers are highly reproducible, locus-specific, multi-allelic and abundant in animal, plant and microbialgenomes [20]. Although generation of SSR markers is a time-consuming, laborintensive and expensive task, several SSR markers have already been developed for M. grisea infecting rice [21][22][23][24]. However, SSRs have not been used to investigate pathogen populations adapted to finger millet.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of genetic diversity in Magnaporthe grisea populations adapted to finger millet using simple sequence repeats (SSRs) markers

Babu

Sharma

Upadhyaya

et al. 2013

Physiological and Molecular Plant Pathology

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Finger millet blast caused by Magnaporthe grisea(anamorph: Pyricularia grisea) is a great threat to finger millet production worldwide. Genetic diversity and population structure of 72M. griseaisolates collected from finger millet (56), foxtail millet (6), pearl millet (7) and rice (3) frommajor crop growing areas inIndiawas studied using 24 SSR markers. None of the SSRs detected polymorphism in the M. grisea isolates from pearl millet. Seventeen SSR markers were polymorphicin the 65 non pearl millet isolates anddetected 105 alleles, of which one was rare, 83 common, 9 frequent and 12 most frequent. A model-based population structure analysis of the genomic data identified two distinct populations with varying levels of ancestral admixtures among the 65M. griseaisolates. Analysis of molecular variance (AMOVA)indicated that 52% of the total variation among the isolates used in this study was due to differences between the pathogen populations adapted to different hosts, 42% was due to differences in the isolates from the same host, and the remaining 6% due to heterozygosity within isolates. High genetic variability present in M. grisea isolates calls for the continuous monitoring of M. grisea populations anticipating blast resistance breakdown in finger millet cultivars grown in India. Key words:Genetic diversity, Simple sequence repeats, Magnaporthe grisea, Eleusine coracana Highlights: Seventeen of the 24 SSR markers were polymorphic and detected 105 alleles in the 65Magnaporthegriseaisolates.  Cluster analysis of SSR data classified the isolates into three major groups that corresponded with the host specificity.  A model-based population structure analysis identified two distinct populations with varying levels of ancestral admixtures.Kiran Babu et al., 2013 Physiological and molecular plant pathology 3 IntroductionFinger millet (Eleusine coracana L. Gaertn) is a widely grown grain cereal in the semi- grisea populations adapted to finger millet, foxtail millet, pearl millet and rice. Material and Methods Pathogen isolatesBlast infected (leaf, neck and finger) samples of finger millet, foxtail millet and rice were collected from Vizianagaram, Patancheru, and Nandyal in Andhra Pradesh, Mandya and Naganahalli in Karnataka, and Dholi in Bihar, India during 2008-10 rainy seasons (Table 1).In addition, seven M. grisea isolates from four major pearl millet growing states in IndiaRajasthan, Haryana, Maharashtra and Uttar Pradesh [25] were also included in this study (Table 1). Isolations of M. grisea were made from the blast-infected tissue on oatmeal agar medium (rolled oats 50 g, agar 15 g, distilled water 1 L) and incubated at 25±1°C for 15 days.After incubation, a dilute spore suspension (3×10 3 spores/ml) was prepared in sterile doubledistilled water and plated onto 4% water agar in Petri plates. Single germinating conidia were marked after 10-12 h of incubation under a microscope and transferred to test tubes containing oatmeal agar for further studies. Isolation of genomic DNAKiran Babu et al., 2013...

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Development of microsatellite markers for the genetic analysis of Magnaporthe grisea

Cited by 31 publications

References 16 publications

Diversidade genética por marcadores moleculares em Fusarium oxysporum f. sp. cubense no Estado de Santa Catarina

Diversidade genética por marcadores moleculares em Fusarium oxysporum f. sp. cubense no Estado de Santa Catarina

Evaluating Short Sequence Repeats (SSRs) Markers in Sordaria fimicola by High Resolution Melt Analysis

Evaluation of genetic diversity in Magnaporthe grisea populations adapted to finger millet using simple sequence repeats (SSRs) markers

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