Taxa de multiplicação e efeito residual de diferentes fontes de citocinina no cultivo in vitro de Hancornia speciosa Gomes

Soares, Fernanda Pereira; Paiva, Renato; Alvarenga, Amauri Alves de; Nery, Fernanda Carlota; Vargas, Daiane Peixoto; Silva, Douglas Ramos Guelfi

doi:10.1590/s1413-70542011000100019

Cited by 27 publications

(27 citation statements)

References 13 publications

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“…Soarez et al, 2011;Souza et al, 2010;Jacyna y Barnard, 2008) en estos estudios se han evidenciado efectos en la etiolación, formación de brotes y la pérdida de dominancia apical. Los resultados del presente estudio corroboran lo indicado por Soares et al (2011);Souza et al (2010); Jacyna y Barnard (2008) y se demuestra que las plántulas micropropagadas tienen una mayor producción de brotes y reducen la dominancia apical durante la fase in vitro. Souza et al (2010) reporta que la BAP es la citoquinina que mayor efecto residual tiene en el proceso de micropropagación de Hancornia speciosa, con una producción de brotes 5 veces mayor; en el caso de C. officinalis se encontró una menor diferencia entre las plántulas inducidas y el control (plantas generadas a partir de semillas); sin embargo, las diferencias encontradas podrían ser atribuibles a efectos residuales de la BAP, en concordancia con lo encontrado por Souza et al (2010).…”

Section: Discussionunclassified

Patrones de crecimiento de Cinchona officinalis in vitro y ex vitro; respuestas de plántulas micropropagadas y de semillas.

Espinosa¹,

Rios²

2017

REMCB

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RESUMEN.-El uso de herramientas biotecnológicas como la micropropagación se constituye en una alternativa de reproducción de especies amenazadas y con tamaños poblacionales reducidos. Sin embargo, uno de los problemas críticos en el uso de la micropropagación como herramienta de reproducción es la calidad de las plántulas resultantes en cuanto a su crecimiento y vigor. En el presente trabajo se evalua los efectos de la micropropagación sobre los patrones de crecimiento y sobrevivencia de plán-tulas in vitro de Cinchona officinalis L., una especie que ha sido fuertemente impactada por procesos de tala dentro de bosques naturales durante la época de la colonia. Se realizó un monitoreo de un total de 120 plántulas in vitro y 1988 plántulas ex vitro por 8 meses a partir del último repique. Adicionalmente, en cada plántula se contabilizó la cantidad de brotes axilares. Los resultados obtenidos mostraron un efecto remanente de los procesos de micropropagación, los cuales inicialmente inciden en la cantidad de brotes de las plántulas y en el crecimiento; sin embargo, este efecto no influye de forma negativa en la sobrevivencia de las plántulas durante la fase ex vitro. PALABRAS CLAVES: conservación, cultivo in vitro, efectos micropropagación, especies forestales, sobrevivencia de plántulas ABSTRACT.-The use of biotechnological tools such as micropropagation constitutes an alternative to reproduction of endangered species and with small population sizes. However, one of the critical issues in the use of micropropagation as a reproduction tool is the quality of seedlings with regard to growth and vigor. In this paper we evaluated the effects of micropropagation on patterns of growth and survival of in vitro seedlings of Cinchona officinalis L. This species has been heavily impacted by logging processes in natural forests during colonial times because of their medical use. We monitored during 8 months after the last peal a total of 120 in vitro and 1988 ex vitro seedlings. Additionally, we measured in each seedling the number of axillary buds. The results showed that there is a residual effect of micropropagation process, which initially affects the number of shoots of seedlings and growth. However, this effect had a negative influence on seedling survival during ex vitro phase.

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Section: Discussionunclassified

Patrones de crecimiento de Cinchona officinalis in vitro y ex vitro; respuestas de plántulas micropropagadas y de semillas.

Espinosa¹,

Rios²

2017

REMCB

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“…These explants were grown in culture medium composed by Wood Plant Medium (WPM) basal salts (Lloyd & Mccown, 1980) agar, as established by Soares et al (2011).…”

Section: Plant Materialsmentioning

confidence: 99%

“…and jams (Soares et al, 2011). Since it is an iron-rich fruit and a vitamin C source, the mangaba fruit occupies a prominent position among functional foods (Silva Júnior et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Since it is an iron-rich fruit and a vitamin C source, the mangaba fruit occupies a prominent position among functional foods (Silva Júnior et al, 2007). For all these characteristics, this species has been the target of predatory extraction, which has led to a decline in H. speciosa population in its native areas (Soares et al, 2011). In addition, the recalcitrance of its seeds is another detrimental factor for the availability of the species, since it prevents conventional storage and its later use (Barros et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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<b>Behavior of lateral buds of <i>Hancornia speciosa</i> after cryopreservation by encapsulation-vitrification

Prudente

Paiva

Nery

et al. 2017

Acta Sci. Biol. Sci.

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ABSTRACT. Hancornia speciosa is a fruitful species from Cerrado biome with high economic potential. However, the intense and disordered extractivism have caused a reduction of its population in its endemic area. In addition, seed recalcitrance negatively affects the conventional conservation of the species. Aiming to find alternatives that enable the long-term conservation of this species, the study's objective was to assess the behavior of lateral bud's regrowth after cryopreservation procedures by encapsulation-vitrification technique. Sodium alginate capsules containing lateral buds were pre-cultured in liquid WPM supplemented with 1.0 M glycerol, and subsequently exposed to different concentrations of sucrose (0.3; 0.75 and 1.0 M) for 24 or 48 hours. The capsules were subjected to dehydration in silica gel or airflow hood for 0, 1, 2 and 3 hours before different incubation times in PVS2 (0, 15, 30, 60 and 120 minutes) at 0°C. A high regeneration percentage of lateral buds was observed after cryopreservation of capsules treated with 0.75 M sucrose plus 1.0 M glycerol (24 hours), associated with dehydration in an airflow hood (1 hour) and immersion in PVS2 (15 minutes). Encapsulation-vitrification allowed the long-term conservation, and provided high plant material survival rates after cryopreservation of Hancornia speciosa sensitive explants.Keywords: Mangabeira, recalcitrant species, long-term conservation, dehydration.Comportamento de gemas laterais de Hancornia speciosa após criopreservação por encapsulamento-vitrificação RESUMO. Hancornia speciosa é uma frutífera do Cerrado com elevado potencial econômico. Entretanto, o extrativismo desordenado causou a redução populacional em sua área endêmica. Além disso, a recalcitrância da semente afeta negativamente sua conservação convencional. Buscando alternativas de conservação para essa espécie, objetivou-se avaliar a regeneração das gemas laterais após a técnica de encapsulamento-vitrificação. Cápsulas de alginato de sódio contendo gemas laterais foram pré-cultivadas em meio WPM acrescido de 1,0 M de glicerol e, posteriormente, imersas em diferentes concentrações de sacarose (0,3; 0,75 e 1,0 M) por 24 ou 48 horas. As cápsulas foram submetidas à desidratação em sílica gel ou em fluxo laminar por 0, 1, 2 e 3 horas antes de sua incubação em diferentes tempos de PVS2 (0, 15, 30, 60 e 120 minutos). Elevada porcentagem de regeneração de gemas laterais foi observada após a criopreservação de cápsulas tratadas com 0,75 M de sacarose + 1,0 M de glicerol por 24 horas, associado com a desidratação em fluxo laminar (1 hora) e imersão em PVS2 (15 minutos). O encapsulamento-vitrificação é eficiente para a conservação de longo prazo e permite a obtençao de altas taxas de sobrevivência após a criopreservação de explantes sensíveis (gemas laterais) de Hancornia speciosa.Palavras-chave: Mangabeira, espécie recalcitrante, conservação em longo prazo, desidratação.

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“…Thus, they are consumed as fresh fruits and also used as raw material for the production of various products (Soares et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Hygroscopic trend of lyophilized ‘mangaba’ pulp powder

Conegero

Ribeiro

Monteiro

et al. 2017

Rev. bras. eng. agríc. ambient.

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A B S T R A C TMangaba is a widely-consumed fruit in the Northeast of Brazil, which is usually exploited through extractivism. This fruit is rich in various nutrients, especially in vitamin C, with pleasant taste and aroma. The lyophilization process transforms these fruits into amorphous powders, which must be analyzed regarding their properties and hygroscopic trend. Thus, the objective of this study was to characterize and evaluate the physico-chemical properties of adsorption isotherms of the lyophilized 'mangaba' pulp powder, with addition of maltodextrin (DE 20). The pH, titratable acidity, soluble solids, ascorbic acid and water activity were analyzed. Regarding the isotherms, the mathematical models of GAB, BET, Oswin, and Henderson were used at temperatures of 25, 30, 35 and 40 °C. The obtained powder presented pH of 3.14, titratable acidity of 1.95 mg of citric acid 100g -1 of powder, soluble solid contents of 99 ºBrix, ascorbic acid content of 55.97 mg 100g -1 and water activity of 0.16. Henderson was the mathematical model that best fitted the data of the adsorption isotherms at the four evaluated temperatures, with average errors ranging from 5.76 to 9.70% and R 2 from 0.9974 to 0.9995. Comportamento higroscópico do pó da polpa de mangaba liofilizada R E S U M OA mangaba é um fruto bastante consumido no Nordeste do Brasil e ainda explorado de maneira extrativista. Os frutos são ricos em vários nutrientes, notadamente em vitamina C, além de possuírem sabor e aroma agradáveis. A liofilização permite a obtenção de pós de natureza amorfa que necessitam de análise de suas propriedades e comportamento higroscópico. O objetivo do presente trabalho foi realizar a caracterização físico-química e avaliação das isotermas de adsorção do pó da polpa de mangaba liofilizada adicionada de maltodextrina (DE 20

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Taxa de multiplicação e efeito residual de diferentes fontes de citocinina no cultivo in vitro de Hancornia speciosa Gomes

Cited by 27 publications

References 13 publications

Patrones de crecimiento de Cinchona officinalis in vitro y ex vitro; respuestas de plántulas micropropagadas y de semillas.

Patrones de crecimiento de Cinchona officinalis in vitro y ex vitro; respuestas de plántulas micropropagadas y de semillas.

<b>Behavior of lateral buds of <i>Hancornia speciosa</i> after cryopreservation by encapsulation-vitrification

Hygroscopic trend of lyophilized ‘mangaba’ pulp powder

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