Viabilidade do pólen de laranjas doces em diferentes condições de armazenamento

Pio, Leila Aparecida Salles; Ramos, José Darlan; Pasqual, Moacir; Junqueira, Keize Pereira; Santos, Flávia Carvalho; Rufini, José Carlos Morais

doi:10.1590/s1413-70542007000100022

Cited by 22 publications

(26 citation statements)

References 5 publications

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“…Boron and calcium are essential for the onset of the intine prolongation and in vitro pollen tube formation (BREWBAKER; KWACK, 1963). Pio et al (2007) and Ramos et al (2008) observed that the boron concentration of 200 mg L -1 stimulated the germination of pollen grains of citrus cultivars.…”

Section: Resultsmentioning

confidence: 97%

In vitrogermination and viability of pollen grain of coconut accessions

Moura¹,

Machado²,

Lédo³

2015

Revista Ciência Agronômica

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-Storage as a means of maintaining the pollen viability is important for the preservation of the genetic variability, facilitates the exchange of germplasm and greatly contributes to the generation of variability obtained from artificial crosses, increasing the efficiency of breeding programs. The objective of this study was to select different culture media for the in vitro germination of pollen grain of dwarf and tall coconut accessions, as well as to determine the viability of pollen grain at room temperature conditions. For this purpose, Brazil Green Dwarf (BGD) and Brazilian Tall (BRA) pollen grains derived from the Coconut Active Germplasm Bank of Embrapa Coastal Tablelands Sergipe were used. To evaluate the effect of different culture media on the in vitro germination of pollen grains of anão verde do Brasil de Jiqui (AVeBrJ) and gigante do Brasil Praia do Forte (GBrPF) accessions, they were inoculated on to Petri dishes containing 2 ml of culture media. The pollen viability was assessed by staining with 1% acetic carmine and in vitro germination at 0, 24, 48 and 72 hours. The culture medium of Lora is suitable to assess the in vitro germination of pollen grain of the AVeBrJ and GBrPF accessions. The pollen grain of the AVeBrJ accession showed intermediate viability (66.87%) at room temperature up to 23.14 hours by in vitro germination. The pollen grain of the GBrPF accession showed high viability, above 70%, at room temperature up to 120 hours by in vitro germination.

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Section: Resultsmentioning

confidence: 97%

In vitrogermination and viability of pollen grain of coconut accessions

Moura¹,

Machado²,

Lédo³

2015

Revista Ciência Agronômica

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“…As demais interações foram altamente significativas, a 1% de probabilidade, em todas as épocas de observação, conforme se observa na Tabela 1. Esses resultados confirmam que a percentagem de germinação é dependente de diversos fatores, principalmente, aqueles relacionados com o armazenamento (Pio et al, 2007).…”

Section: Resultsunclassified

“…Portanto, os resultados mencionados estão de acordo com os encontrados na literatura, ou seja, com o passar do tempo observou-se redução significativa da viabilidade dos grãos de pólen armazenados e a decisão com respeito ao méto-do a ser empregado no armazenamento dependerá do objetivo e do tempo exigido para seu alcance, ou seja, a longo ou a médio prazo (Pio, 2003).…”

Section: Resultsunclassified

“…De acordo com Pio et al (2007), os resultados desse procedimento serviram de referência para a comparação da percentagem de grãos de pólen germinados após armazenamento. No entanto, não foi necessário avaliar o efeito do armazenamento dos grãos de pólen, à temperatura ambiente, por oito semanas, visto que, na abertura floral, o grão de pólen necessita estar viável e, com o passar do tempo, a viabilidade vai diminuindo e vai-se reduzindo sua eficiência na fertilização (Souza et al, 2002).…”

Section: Methodsunclassified

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Conservação de grãos de pólen de mamoneira a baixas temperaturas

2012

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Conservation of castor bean pollen at low temperaturesThe objective of storing pollen is to preserve material for future use, providing optimal conditions to maintain their original germination, vigor and genetic integrity. The aim of this study was to investigate the germination of castor bean (Ricinus communis L.) pollen after storage at low temperatures. For this purpose, pollen of the cultivars IAC 80 and AL Guarany 2002 were used and stored at four different temperatures, -196; -72; -18; and 4°C, for a period of 60 days. The pollen viability was assessed weekly for five weeks, with a last evaluation performed after eight weeks of storage using the in vitro germination test. Culture medium containing 10 gL -1 agar, 100 gL -1 sucrose, 0.004 g L -1 boric acid, pH 6.0 was prepared and poored into cavity slides, where the pollen was evenly distributed over the surface of the medium. The plates were incubated at 20°C in BOD. The experiment was arranged in a 2x4 factorial completely randomized design (2 cultivars x 4 temperatures) for each storage time, with 100 pollen grains analyzed per repetition, totaling six of each treatment. There was significant difference between treatments for the cultivar x temperature interaction in the fifth week of observation (p<0.05). Other cultivar x temperature interactions were highly significant at all times of observation (p<0.01). It is therefore concluded that the use of ultra low temperatures allows the maintenance of castor bean pollen viability until the fifth week of storage.

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“…A viabilidade do grão de pólen, que é uma medida de fertilidade masculina, pode ser determinada por método direto, com uso de técnicas de indução à germinação in vitro (Pio et al 2007) ou in vivo (Ferreira et al 2007) e, por método indireto baseado em parâmetros citológicos, como a coloração (Colas & Mercier 2000). No emprego da coloração, é frequente o uso de corantes nucleares como carmim acético e azul de anilina, os quais colorem apenas pólens funcionais.…”

Section: Introductionunclassified

Conservação e germinação in vitro de pólen de milho (Zea mays subsp. mays)

2011

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-(Conservation and in vitro germination of pollen of maize (Zea mays subsp. mays)). The storage of pollen can be considered an important tool for maize breeding programs, allowing to preserve, under artificial conditions, the viability of male gametes and extend the possibilities of crossings regardless of flowering time of parental varieties. This study aimed to evaluate the culture media for in vitro germination of corn pollen and analyze storage conditions. To examine viability, six different culture media containing sucrose, boric acid, calcium chloride and agar were evaluated. For pollen preservation, two temperatures (4 °C and -20 °C) and two agents of pollen dehydration (silica gel and hydrated calcium chloride) were evaluated. The high values of pollen viability up to 30 days of storage indicate that dehydration in silica gel and storage at 4 °C preserve the viability of corn pollen. The culture medium that provided the highest germination rate in vitro was the composition of 0.7% agar, 17% sucrose, 0.01% boric acid and 0.03% calcium chloride hydrate.

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Viabilidade do pólen de laranjas doces em diferentes condições de armazenamento

Cited by 22 publications

References 5 publications

In vitrogermination and viability of pollen grain of coconut accessions

In vitrogermination and viability of pollen grain of coconut accessions

Conservação de grãos de pólen de mamoneira a baixas temperaturas

Conservação e germinação in vitro de pólen de milho (Zea mays subsp. mays)

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