Expressão de marcadores de proliferação celular e apoptose em carcinoma basocelular

Corrêa, Marília de Pádua Dornelas; Ferreira, Ana Paula; Gollner, Ângela Maria; Rodrigues, Michele Fernandes; Guerra, Magno Cunha de Souza

doi:10.1590/s0365-05962009000600006

Cited by 10 publications

(4 citation statements)

References 14 publications

(22 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is due to the antibody's immunoreactivity instability. According to a published study 21 , Ki-67 allows inferences about the time of life of a particular cell, stating only if it is in the cell cycle 21 . Therefore, it is possible that a particular neoplasm has a high proliferation rate and a low percentage of cells positive for this antibody.…”

mentioning

confidence: 99%

Expression of Ki-67 and P16 INK4a in chemically-induced perioral squamous cell carcinomas in mice.

Alves

Ribeiro

Lima

et al. 2016

Rev. Col. Bras. Cir.

View full text Add to dashboard Cite

Objective: to evaluate the influence of INK4a proteins immunohistochemical expressions on the clinical and morphological parameters of perioral squamous cell carcinoma induced with 9,10-dimethyl-1,2-benzanthracene (DMBA) in mice. Methods: we topically induced the lesions in the oral commissure of ten Swiss mice for 20 weeks, determining the time to tumors onset and the average tumor volume up to 26 weeks. In histopathological analysis, the variables studied were histological malignancy grade and the immunohistochemical expression of INK4a proteins. The correlation between variables was determined by application of the Spearman correlation test. Results: the mean time to onset of perioral lesions was 21.1 ± 2.13 weeks; mean tumor volume was 555.91 ± 205.52 mm3. Of the induced tumors, 80% were classified as low score and 20% high score. There was diffuse positivity for Ki-67 in 100% of lesions -Proliferation Index (PI) of 50.1 ± 18.0. There was a strong direct correlation between Ki-67 immunoreactivity and tumor volume (R = 0.702) and a low correlation with the malignancy score (R = 0.486). The P16INK4a protein expression was heterogeneous, showing a weak correlation with tumor volume (R = 0.334). There was no correlation between the immunohistochemical expression of the two proteins studied. Conclusion: in an experimental model of DMBA-induced perioral carcinogenesis, tumor progression was associated with the tumor proliferative fraction (Ki-67 positive cells) and with tumor histological grading, but not with P16 INK4a expression.Keywords: Carcinogenesis. 9,10-Dimetil-1,2-benzanthracene. Immunohistochemistry. Ki-67 Antigen. Genes, p16. MTSI, CDK4I or CDKN2) located on chromosome 9p, locus 21, involved in cell cycle progression blocking process. It is inactive in a wide range human malignancies. The loss of p16 INK4a immunoexpression has been observed in the early stages of oral carcinogenesis and has been considered a molecular event of significant value in the prognostic analysis of such tumors 9,10 . This study evaluated the influence of Ki-67 and p16 proteins immunohistochemical expression on morphological parameters (mean tumor volume and histological malignancy grade) of DMBA-induced perioral SCC in mice. In addition, it sought to verify the existence of correlation between the immunoreactivity of p16 and Ki-67 proteins. METHODSThe development of the study had the approval of the Ethics in Research Committee of the Universidade Tiradentes -Aracaju / SE, with protocol number 191208. Animals and chemical carcinogenesis induction procedureWe used ten Swiss mice without distinction between gender, from the vivarium of the Universidade Tiradentes, with a body mass of about 150 ± 30g (Average age 100 days).We induced the oral lesions in the mice left oral commissure by the topical application of 9,10-dimethyl-1,2-benzanthracene (DMBA), diluted to 0.5% in acetone, on a weekly basis in three alternate days for 20 weeks 11 . After this period, the animals were kept under observation for six weeks, and we duly regis...

show abstract

mentioning

confidence: 99%

Expression of Ki-67 and P16 INK4a in chemically-induced perioral squamous cell carcinomas in mice.

Alves

Ribeiro

Lima

et al. 2016

Rev. Col. Bras. Cir.

View full text Add to dashboard Cite

show abstract

“…Apoptosis is a protective mechanism for cancer which leads damaged cells to death and prevents their expansion. Alterations in apoptotic and antiapoptotic proteins or imbalance between cell growth and apoptosis cause susceptibility to cancer [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Caspase-3, p53 and Bcl-2 expression in basal cell carcinoma of the eyelid

Koyun¹,

Karadağ²,

Özkanlı³

et al. 2020

pdia

View full text Add to dashboard Cite

Introduction: Eyelid tumours mostly originated from skin and its appendeges. External carcinogens like UV radiation causes cell damages in the eyelid skin and contributes to carcinogenesis. Apoptosis is a very important mechanism to prevent these damage and probable neoplatic change. Aim: To compare caspase-3, p53 and Bcl-2 levels between patients with basal cell carcinoma (BCC) of the eyelid and healthy individuals. Material and methods: Pathology archives from October 2012 to April 2015 were scanned for BCC biopsies of the eyelid and tissue removed during blepharoplasty and entropion procedures. A total of 36 specimens were found. The specimens were divided into two groups: BCC group and controls (consisting of eyelid tissue removed during routine blepharoplasty). The pathology specimens were then stained using p53, Bcl-2 and caspase-3 stains and the intensity of staining was graded on a 0-3 scale. Results: Samples from a total of 36 patients were included in the study. Eighteen (50.0%) patients were female. There were 13 patients in the BCC group and 23 patients in the control group. The mean age was 66.0 ±10.8 years in the BCC group, and 65.61 ±11.22 years in the control group. The caspase-3 staining was lower in the BCC group than in the control group. No significant differences were found between the BCC group and the control group in terms of p53 levels or Bcl-2 levels (both of them, p = 1.000). Conclusions: The caspase-3 level was lower in the BCC group. This result suggests that these enzymes can play a significant role in carcinogenesis of eyelid BCC.

show abstract

“…However, the ability to promote apoptosis represents a form of primary cellular resistance to genomic and metabolic alterations linked to malignant neoplastic initiation, promotion, and proliferation. 3 Thus, different expression profiles of proteins linked to proliferation and apoptosis, such as p53, ki67, survivin, and NFκB pathway (p105), may characterize the clinical subtypes of BCC and the pattern of recurrent cases relative to the adjacent normal epithelium.…”

mentioning

confidence: 99%

“…Survivin, on the other hand, is expressed in several neoplasms, evidencing resistance to apoptosis. 3…”

mentioning

confidence: 99%

Patterns of proliferation and apoptosis in different subtypes of basal cell carcinoma, adjacent epidermis, and recurrent forms

Lima

Miola

Marques

et al. 2019

An. Bras. Dermatol.

View full text Add to dashboard Cite

Expressão de marcadores de proliferação celular e apoptose em carcinoma basocelular

Cited by 10 publications

References 14 publications

Expression of Ki-67 and P16 INK4a in chemically-induced perioral squamous cell carcinomas in mice.

Expression of Ki-67 and P16 INK4a in chemically-induced perioral squamous cell carcinomas in mice.

Caspase-3, p53 and Bcl-2 expression in basal cell carcinoma of the eyelid

Patterns of proliferation and apoptosis in different subtypes of basal cell carcinoma, adjacent epidermis, and recurrent forms

Contact Info

Product

Resources

About