Caspase-3, p53 and Bcl-2 expression in basal cell carcinoma of the eyelid

Abstract

Introduction: Eyelid tumours mostly originated from skin and its appendeges. External carcinogens like UV radiation causes cell damages in the eyelid skin and contributes to carcinogenesis. Apoptosis is a very important mechanism to prevent these damage and probable neoplatic change. Aim: To compare caspase-3, p53 and Bcl-2 levels between patients with basal cell carcinoma (BCC) of the eyelid and healthy individuals. Material and methods: Pathology archives from October 2012 to April 2015 were scanned for BCC biopsies of the eyelid and tissue removed during blepharoplasty and entropion procedures. A total of 36 specimens were found. The specimens were divided into two groups: BCC group and controls (consisting of eyelid tissue removed during routine blepharoplasty). The pathology specimens were then stained using p53, Bcl-2 and caspase-3 stains and the intensity of staining was graded on a 0-3 scale. Results: Samples from a total of 36 patients were included in the study. Eighteen (50.0%) patients were female. There were 13 patients in the BCC group and 23 patients in the control group. The mean age was 66.0 ±10.8 years in the BCC group, and 65.61 ±11.22 years in the control group. The caspase-3 staining was lower in the BCC group than in the control group. No significant differences were found between the BCC group and the control group in terms of p53 levels or Bcl-2 levels (both of them, p = 1.000). Conclusions: The caspase-3 level was lower in the BCC group. This result suggests that these enzymes can play a significant role in carcinogenesis of eyelid BCC.

Protective Effect of Pycnogenol against Methotrexate-Induced Hepatic, Renal, and Cardiac Toxicity: An In Vivo Study

“…Several stimuli, including hormones, cytokines, growth factors, and immunological responses, can influence the apoptosis process. The presence of cells expressing active caspase 3 is regarded as a marker of apoptotic activation [53,54]. Furthermore, it has been demonstrated that the p53 proteins play a critical regulatory role in apoptosis [40,53].…”

Evaluation of MMP2, Caspase-3 and C-Kit Expression on Thyroid Papillary Cancer Prognosis

“…The tissue sections taken to poly-L-lysine coated slides were stained in CD117/c-kit (Biocare Medical PME296AA) 1:50, MMP2 (GeneTex GTX14577) 1:200, caspase-3 (GeneTex GTX73090) 1:1000 antibody dilutions by immunohistochemistry (IHC) method after deparafinization according to the reference (Koyun et al, 2020).…”