Methanogenic potential and microbial community of anaerobic batch reactors at different ethylamine/sulfate ratios

Abstract

-Methylamine and sulfate are compounds commonly found in wastewaters. This study aimed to determine the methanogenic potential of anaerobic reactors containing these compounds and to correlate it with their microbial communities. Batch experiments were performed at different methylamine/sulfate ratios of 0.71, 1.26 and 2.18 (with respect to mass concentration). Control and experimental runs were inoculated with fragmented granular sludge. The maximum specific methane formation rates were approximately 2.3 mmol CH 4 L -1 g TVS -1 day -1 for all conditions containing methylamine, regardless of sulfate addition. At the end of the experiment, total ammonium-N and methane formation were proportional to the initial concentrations of methylamine. In the presence of methylamine and sulfate, Firmicutes (46%), Deferribacteres (13%) and Proteobacteria (12%) were the predominant phyla of the Bacteria domain, while Spirochaetes (40%), Deferribacteres (17%) and Bacteroidetes (16%) predominated in the presence of methylamine only. There was no competition for methylamine between sulfate-reducing bacteria and methanogenic archaea.

COD/sulfate ratio does not affect the methane yield and microbial diversity in anaerobic digesters

“…The effect of sulfate addition on methane production in TMA-amended microcosms is remarkable, as this compound is not known to be a competitive substrate for SRB. Hence, our results disagree with those ofVich et al (2011), who amended methylamine and sulfate to…”

The "Chemical Oxygen Demand / Total Volatile Acids" Ratio as an Anaerobic Treatability Indicator for Landfill Leachates

“…As this configuration presented good results treating synthetic wastewaters (removal efficiency up to 86%), the AnSBBR started to be studied and developed (Ratusznei et al, 2003;Rodrigues et al, 2003;Cubas et al, 2004;Pinho et al, 2004;Siman et al, 2004;Bezerra et al, 2010;Costabile et al, 2010). The AnSBBR was also tested and optimized with many types of wastewaters, including diluted whey (Damasceno et al, 2007), sewage (Sarti et al, 2007) wastewater of the personal-care industry (Oliveira et al, 2009), biodiesel production effluent (Selma et al, 2010;Bezerra et al, 2010), sucrose-based wastewater (Manssouri et al 2013) among other studies for optimization and improvement of the AnSBBR Silva et al, 2011;Vich et al, 2011).…”