COD/sulfate ratio does not affect the methane yield and microbial diversity in anaerobic digesters

Cetecioglu, Zeynep; Dolfing, Jan; Taylor, Jessica Averitt; Purdy, Kevin J.; Eyice, Özge

doi:10.1016/j.watres.2019.02.038

Cited by 49 publications

(12 citation statements)

References 56 publications

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“…This resulted in predicted maximum total sulfide concentrations of 584, 1193, and 2463 mg S L −1 and maximum undissociated H 2 S concentrations of 181, 247, and 358 mg S L −1 for initial sulfate concentrations of 0.84, 1.68, and 3.34 g S L −1 , respectively. These concentrations are within the wide range of reported sulfide concentrations that trigger toxicity in anaerobic environments (100–1400 mg S L −1 as total sulfide and 50–400 mg S L −1 as H 2 S) (Cetecioglu et al, 2019; Chen et al, 2008, 2014; Giménez et al, 2011; Lu et al, 2016; Maillacheruvu et al, 1993; Zamariolli Damianovic et al, 2016), with the exception of the total sulfide concentration at the 3.34 g S L −1 initial condition, which is well above the range. Thus, it appears that methanogenesis can proceed uninhibited at total sulfide concentrations as high as 2463 mg S L −1 with WAS as the organic substrate.…”

Section: Resultssupporting

confidence: 69%

“…They observed that the sulfide inhibition affects acidogenesis the most, while not having a strong effect on the methanogenic step (Polprasert & Haas, 1995). This has been further shown by Cetecioglu et al who found that the digestion of simple substrates such as acetate, propionate, and butyrate using sulfate acclimated sludge was unaffected by SO 4 2— to‐COD ratios as high as 2 g:g, which indicates that sulfide inhibition had no effect on the acetogens and methanogens in those cultures (Cetecioglu et al, 2019). However, other studies report methanogens are the most inhibited by sulfide toxicity (Oleszkiewicz et al, 1989; Shin et al, 1995).…”

Section: Introductionmentioning

confidence: 68%

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Modeling the anaerobic digestion of wastewater sludge under sulfate‐rich conditions

Piccolo

Goel

Snowling

et al. 2021

Water Environment Research

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Anaerobic digestion (AD) is a biological treatment process to stabilize organic solids and produce biogas. If present, sulfate is reduced to sulfide by anaerobic sulfate‐reducing bacteria and the sulfide can be toxic to anaerobic microorganisms. Here, the effect of high initial sulfate concentration on AD of wastewater sludge was investigated using lab‐scale batch experiments. Additionally, a systematic mathematical modeling approach was applied for insight into the experimental results. Cumulative biogas and methane production decreased with increasing initial sulfate doses (0–3.300 mg S L−1). The correlation between the sulfate dose and methane production was consistent with theoretical predictions and model results, indicating no toxic effect of sulfide on methane production. The carbon dioxide content in the biogas decreased linearly with the increasing sulfate dose, which is consistent with the model‐predicted behavior of the bicarbonate and hydrogen sulfide buffering system. The examined high sulfate concentrations resulted in no clear negative effects on the COD removal or VSS destruction of the wastewater sludge, indicating negligible inhibition by sulfide toxicity. Even considering the possibility of ferrous sulfide precipitation and the low model estimates of residual sulfide concentration the residual sulfide concentration was higher than reported concentrations that trigger process inhibition. Practitioner points The effect of sulfate loading on anaerobic digestion of waste activated sludge was characterized. The stoichiometry of sulfate reduction allows accurate prediction of CH4 loss. High sulfate levels (up to 3300 mg/L as S) did not affect COD/VSS removal. Sulfide formation increases effluent COD; often misinterpreted as sulfide toxicity. Correcting COD for sulfide's contributions is crucial for results interpretation.

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Section: Resultssupporting

confidence: 69%

Section: Introductionmentioning

confidence: 68%

Modeling the anaerobic digestion of wastewater sludge under sulfate‐rich conditions

Piccolo

Goel

Snowling

et al. 2021

Water Environment Research

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“…Nevertheless, control of the manipulation mechanism depends on thermodynamic and metabolic principles: the product mixture is specified by thermodynamic constraints and enzyme availability in pure culture fermentation, while, it is more complicated in mixed culture fermentation because of the wide range of metabolic activities and energetic considerations (Mohd-Zaki et al, 2016). Many studies have been conducted to identify metabolic flux and energy conversions in mixed culture fermentation (Lee et al, 2008;Hoelzle et al, 2014;De Vrieze and Verstraete, 2016;Cetecioglu et al, 2019). Lee et al (2008) evaluated H 2 production from glucose fermentation under several pH conditions, thermodynamically (Lee et al, 2008).…”

Section: Volatile Fatty Acid Composition Shift At the Bioaugmented Reactormentioning

confidence: 99%

Bioaugmented Mixed Culture by Clostridium aceticum to Manipulate Volatile Fatty Acids Composition From the Fermentation of Cheese Production Wastewater

Atasoy

Çetecioğlu

2021

Front. Microbiol.

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Production of targeted volatile fatty acid (VFA) composition by fermentation is a promising approach for upstream and post-stream VFA applications. In the current study, the bioaugmented mixed microbial culture by Clostridium aceticum was used to produce an acetic acid dominant VFA mixture. For this purpose, anaerobic sequencing batch reactors (bioaugmented and control) were operated under pH 10 and fed by cheese processing wastewater. The efficiency and stability of the bioaugmentation strategy were monitored using the production and composition of VFA, the quantity of C. aceticum (by qPCR), and bacterial community profile (16S rRNA Illumina Sequencing). The bioaugmented mixed culture significantly increased acetic acid concentration in the VFA mixture (from 1170 ± 18 to 122 ± 9 mgCOD/L) compared to the control reactor. Furthermore, the total VFA production (from 1254 ± 11 to 5493 ± 36 mgCOD/L) was also enhanced. Nevertheless, the bioaugmentation could not shift the propionic acid dominancy in the VFA mixture. The most significant effect of bioaugmentation on the bacterial community profile was seen in the relative abundance of the Thermoanaerobacterales Family III. Incertae sedis, its relative abundance increased simultaneously with the gene copy number of C. aceticum during bioaugmentation. These results suggest that there might be a syntropy between species of Thermoanaerobacterales Family III. Incertae sedis and C. aceticum. The cycle analysis showed that 6 h (instead of 24 h) was adequate retention time to achieve the same acetic acid and total VFA production efficiency. Biobased acetic acid production is widely applicable and economically competitive with petroleum-based production, and this study has the potential to enable a new approach as produced acetic acid dominant VFA can replace external carbon sources for different processes (such as denitrification) in WWTPs. In this way, the higher treatment efficiency for WWTPs can be obtained by recovered substrate from the waste streams that promote a circular economy approach.

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“…These mixed communities offer the advantage of protecting the microbial species of interest (e.g., metal respires or methanogens) against the toxic environment of the industrial effluent. However, on the other side, mixed communities also result in competition between various bacterial groups, some having better fitness and thermodynamics than the ones of interest for resource recovery (e.g., more thermodynamically adapted sulphate-reducing bacteria vs methanogens or metal oxidisers) (Hoelzle et al 2014;Cetecioglu et al 2019;Tang et al 2019).…”

Section: Barriersmentioning

confidence: 99%

A review of nature-based solutions for resource recovery in cities

Kisser¹,

Wirth²,

Gusseme

et al. 2020

Blue-Green Systems

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Our modern cities are resource sinks designed on the current linear economic model which recovers very little of the original input. As the current model is not sustainable, a viable solution is to recover and reuse parts of the input. In this context, resource recovery using nature-based solutions (NBS) is gaining popularity worldwide. In this specific review, we focus on NBS as technologies that bring nature into cities and those that are derived from nature, using (micro)organisms as principal agents, provided they enable resource recovery. The findings presented in this work are based on an extensive literature review, as well as on original results of recent innovation projects across Europe. The case studies were collected by participants of the COST Action Circular City, which includes a portfolio of more than 92 projects. The present review article focuses on urban wastewater, industrial wastewater, municipal solid waste and gaseous effluents, the recoverable products (e.g., nutrients, nanoparticles, energy), as well as the implications of source-separation and circularity by design. The analysis also includes assessment of the maturity of different technologies (technology readiness level) and the barriers that need to be overcome to accelerate the transition to resilient, self-sustainable cities of the future.

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COD/sulfate ratio does not affect the methane yield and microbial diversity in anaerobic digesters

Abstract: Please refer to published version for the most recent bibliographic citation information. If a published version is known of, the repository item page linked to above, will contain details on accessing it.

Cited by 49 publications

References 56 publications

Modeling the anaerobic digestion of wastewater sludge under sulfate‐rich conditions

Modeling the anaerobic digestion of wastewater sludge under sulfate‐rich conditions

Bioaugmented Mixed Culture by Clostridium aceticum to Manipulate Volatile Fatty Acids Composition From the Fermentation of Cheese Production Wastewater

A review of nature-based solutions for resource recovery in cities

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