Organogenesis from internodal segments of yellow passion fruit

Abstract

Organogenesis induction from internodal segments of yellow passionfruit (Passiflora edulis f. flavicarpa) was obtained in a medium supplemented with 1.0-4.0 mg L-1 benzylaminopurine (BAP). Callus proliferation and adventitious shoot development were analyzed by light and scanning electron microscopy. Callus proliferation and adventitious shoots were observed simultaneously and continuously, with asynchronous development of shoots in the explants. Proliferation of callus was observed in the explant cut ends in all treatments, including the control. The results show that concentrations of 1.0 or 2.0 mg L-1 BAP gave better rate of organogenesis. Higher concentrations were efficient for the induction of adventitious buds and, were, however, detrimental for subsequent shoot development. Growth and rooting of shoots were obtained in half strength medium without growth regulators.

In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast.

“…The asynchronous process of the in vitro organogenesis may be explained by the achievement of competence and determination of different-stage cells. The lacking synchrony in the organogenic process was also reported in P. edulis f. flavicarpa (Dornelas and Vieira 1994;Appezzato-da-Glória et al 1999;Biasi et al 2000).…”

“…The formation of buds in leaf discs was observed after the formation of calluses at the explant ends. The indirect organogenesis in P. cincinnata occurred only in culture medium containing 6-BA regulator, which was also observed in internode segments of P. edulis f. flavicarpa (Biasi et al 2000) and in leaf discs of Passiflora suberosa (Monteiro et al 2000). In P. edulis f. flavicarpa, the direct organogenesis in leaf discs described by Dornelas and Vieira (1994), at 28 days of culture in MS medium with 1.0 mg. L -1 de 6-BA, had a regeneration response of 41.7%.…”

“…In the absence of 6-BA regulator, no bud formation was observed and the higher concentration (2.0 mg.L -1 6-BA) was not favorable to bud formation. For P. edulis f. flavicarpa, 2.0 mg.L -1 6-BA concentration was satisfactory to induce organogenesis, while higher doses at 2.0 mg.L -1 injured bud growth (Dornelas and Vieira 1994;Biasi et al 2000). Kantharaj and Dodd (1990) performed a P. edulis micropropagation experiment in node explants, testing two sucrose concentrations (2 and 3%), absence and presence of coconut water (20%), combining three BAP concentrations (1.0, 2.0 and 3.0 mg.L -1 ).…”

“…Several works on in vitro regeneration have been published on a few Passiflora species (Dornelas and Vieira 1994;Appezzato-da-Glória et al 1999;Monteiro et al 2000;Hall et al 2000;Biasi et al 2000;Reis et al 2003;Becerra et al 2004). In order to fully use these tools, in vitro plant regeneration processes can be assayed and characterized by histological and morphological analysis of plant material.…”

Optimization of in vitro organogenesis in passion fruit (Passiflora edulis f. flavicarpa)

“…f. flavicarpa Deg.) has been obtained from shoot apices or nodal segments cultivation (Drew, 1991;Faria & Segura, 1997a;Monteiro et al, 2000;Reis et al, 2003) or from adventitious buds developed from leaf discs (Dornelas & Vieira, 1994;Appezzato-da-Glória et al, 1999), hypocotyl (Faria & Segura, 1997b), or internodal segments (Biasi et al, 2000). Optimized protocols have been established mainly by using different combinations of plant growth regulators, such as BAP and IBA (Kawata et al, 1995), BAP and NAA (Dornelas & Vieira, 1994), BAP and IAA (Faria & Segura, 1997b), and different salt solutions (Faria & Segura, 1997b;Monteiro et al, 2000).…”