Optimization of in vitro organogenesis in passion fruit (Passiflora edulis f. flavicarpa)

Abstract

In vitro organogenesis of passion fruit was studied by the induction of adventitious buds from leaf discs in culture media supplemented with benzyladenine (BAP) or thidiazuron (TDZ). To minimize adverse effects of ethylene accumulation on shoot development, silver nitrate (AgNO 3 ) was added to the induction media. Both BAP (0; 2.2; 4.4; 6.6 µmol L -1 ) and TDZ (0; 1.1; 2.2; 3.4 µmol L -1) were effective in promoting shoot development. Although no significant differences were detected using AgNO 3 (23.5 µmol L -1 ), buds grown in AgNO 3 -supplemented media were more vigorous. The number of explants with buds obtained using TDZ and AgNO 3 -supplemented media (5.6) were higher than those obtained using BAP and AgNO 3 (3.0). MSM + giberrellic acid (GA 3 ), MSM + coconut water, and ½ MSM culture media were tested for shoot bud elongation, incubated in flasks covered with either non-vented or vented lids. Best results were obtained by culturing buds in MSM + coconut water media in flasks covered with vented lids. Plantlets transferred to MSM + indol butyric acid (IBA) media rooted in a 30-day period. Passion fruit organogenesis was enhanced by using TDZ and AgNO 3 for bud induction. Transferring the buds to MSM + coconut water media and incubating in flasks with vented lids favored shoot elongation and plantlet development.

Effect of plant growth regulators and antioxidants on in vitro plant regeneration and callus induction from leaf explants of purple passion fruit (Passiflora edulis Sims)

“…Adventitious shoot production from leaf segment has been previously observed in P. alata (Pinto et al 2010;Rodriguez et al 2007). The use of cytokinins for in vitro organogenesis in Passiflora species has also been reported with induction of adventitious buds in response to BAP alone or in association with NAA, TDZ or kinetin (Becerra et al 2004;Dornelas and Vieira 1994;Hall et al 2000;Trevisan and Mendes 2005). Shoots from root explants of P. cincinnata and P. edulis grown in MS medium with 2.89 uM GA 3 were elongated about 2 cm after 10 days in culture .…”

“…Pinto et al (2010) reported that in vitro organogenesis induction of sweet passion fruit occurred more efficiently when hypocotyl segment-derived explants were cultured in MS medium supplemented with BAP and AgNO 3 under 16-h photoperiod. Trevisan et al (2005) also reported that the bud induction and shoot development of P. edulis Sims. F. flavicarpa was enhanced TDZ and AgNO 3 -supplemented media.…”

“…Passiflora species are generally considered to produce ethylene at high rates, and the tissue culture medium supplementation with ethylene action inhibitors has proved to improve the bud formation and enhance shoot growth as well as differentiation (Faria and Segura 1997;Trevisan and Mendes 2005). Pinto et al (2010) reported that in vitro organogenesis induction of sweet passion fruit occurred more efficiently when hypocotyl segment-derived explants were cultured in MS medium supplemented with BAP and AgNO 3 under 16-h photoperiod.…”

Concentrations of Silver Nitrate in the In Vitro Development and Conservation of <i>Passiflora gibertii</i> N. E. Brown

“…In order to minimize the effect of the accumulation of ethylene, in the development of passion fruit shoots, [8] added silver nitrate to the culture media supplemented with benzyladenine (BAP) or thidiazuron (TDZ) and verified that adventitious buds developed in culture media with supplemented AgNO 3 were more vigorous.…”

Effect of coconut water on growth of olive embryos cultured in vitro

“…The same authors concluded that the use of coconut water help to prolong the sub-culturing time and produced highly robust plants which were more able to survive in greenhouse condition. TREVISAN et al (2005) also demonstrated the advantage of coconut water for stem elongation and plant development in passion fruit. VILLA et al (2010) working with the olive cultivar 'Ascolano 315' reported that 25ml L -1 of coconut water associated with 500µg L -1 of BAP generated plantlets with higher shoot length and heavier fresh biomass than in plantlets cultivated without these compounds.…”