Rapid serum agglutination and agar gel immunodiffusion tests associated to clinical signs in rams experimentally infected with Brucella ovis

Nozaki, Cristiane Nakada; Lira, Nair Silva Cavalcanti de; Filho, Otávio Augusto; Azevedo, H. C.; Rodello, Leandro; Dasso, Maurício Gautério; Bicudo, Sony Dimas; Antunes, João Marcelo Azevedo de Paula; Megid, Jane

doi:10.1590/s0103-84782011000800023

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…When comparing to AGID the rBP26 iELISA generated less clear results ( Figure 5). These results are likely to be a consequence of the unreliable performance of AGID (Xavier et al, 2011), which does not correlate well with actual infection since rams that eliminate B. ovis in the semen can be AGID negative either in experimental (Nozaki et al, 2011) or natural infections (Costa et al, 2012). The rBP26 iELISA developed in this study had a sensitivity of 100% with serum from experimentally infected rams, which contrasts with the rBP26 iELISA previously developed by Zygmunt et al (2002) that employed a partially purified rBP26 as antigen, and had a sensitivity of 81.8%.…”

Section: Resultsmentioning

confidence: 99%

Indirect ELISA for diagnosis of Brucella ovis infection in rams

França

Mol

Costa

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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Indirect ELISA for diagnosis of Brucella ovis infection in rams [ELISA indireto ABSTRACTBrucella ovis is a major cause of epididymitis in sexually mature rams, resulting in subfertility, infertility, and economic losses for the sheep industry worldwide. The aim of this study was to develop an indirect ELISA (iELISA) using recombinant proteins, namely rBoP59 and rBP26, as antigens for serological diagnosis of B. ovis infection. The BoP59 and BP26 recombinant proteins were expressed in E. coli and purified by affinity chromatography. Antigenicity was tested by Western blot and iELISA. Standardization of iELISA was performed with 500ng and 1µg BoP59 and rBP26 per well, testing serum from uninfected and experimentally infected rams. rBP26 was effective in distinguishing positive from negative rams. The rBP26 iELISA developed in this study is the first to use a completely purified rBP26 as antigen resulting in high sensitivity (100%) and specificity (90.2%), and an overall accuracy equal to 1.0.

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Section: Resultsmentioning

confidence: 99%

Indirect ELISA for diagnosis of Brucella ovis infection in rams

França

Mol

Costa

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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“…Previous studies have demonstrated that seropositivity for B. ovis by AGID is intermittent throughout the course of infection. Indeed experimentally infected rams that shed B. ovis in the semen may have no antibody detectable by AGID [ 13 , 14 ]. Interestingly, all PCR-positive animals in this study were also serologically positive by ELISA, while none of the AGID-positive animals were positive by any other diagnostic method.…”

Section: Discussionmentioning

confidence: 99%

Serologic and molecular evidence of Brucella ovis infection in ovine and caprine flocks in the State of Minas Gerais, Brazil

et al. 2016

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BackgroundBrucella ovis infection is one of the leading causes of sub fertility and infertility in ovine, been characterized mainly by epididymitis, orchitis and testicular atrophy in rams. This study aimed to determine the frequency of B. ovis positivity in rams and goats flocks in the State of Minas Gerais, Brazil, by agarose gel immunodiffusion (AGID), ELISA, Rose Bengal, PCR and bacteriological isolation as diagnostic tools.FindingsSerum and urine samples were collected from properties with sheep or goat flocks, or from properties with mixed flock. Out of 50 sheep flocks, 6 % (3/50) were seropositive by AGID while 4 % (2/50) were positive by urine PCR for B. ovis. Out of five goat farms, 20 % (1/5) were seropositive for B. ovis by AGID. Mixed flock farms had 11.1 % (2/18) of positivity by AGID. By ELISA, 19.5 % (8/41) of sheep properties and 61.1 % (11/18) of the properties with mixed flocks were positive for B. ovis. No samples were positive in the test of Rose Bengal, ruling out exposure to smooth LPS Brucella species (particularly Brucella melitensis) and indicating that the positive in the ELISA was associated with Brucella spp. LPS rough (presumably B. ovis). No urine sample from sheep or goat was positive by bacteriological isolation.ConclusionsOur results demonstrate serologic or molecular evidence of B. ovis infection in several rams and billy goats from meso-regions of the State of Minas Gerais, Brazil. Also, this study report the indirect ELISA as an important tool for the diagnosis of B. ovis infection, as indirect ELISA in this study demonstrated to be the most sensitive diagnostic method adopted.

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“…In females, it can trigger placentitis, miscarriages, birth of weak and stillborn lambs, among other changes. In rams, the development of epididymitis and orchitis is characteristic (GREVE et al, 2011;NOZAKI et al, 2011).…”

Section: Seroprevalence Of Ovine Brucellosis In the Microregion Of Tementioning

confidence: 99%

Seroprevalence of ovine brucellosis in the microregion of Teresina, Piauí, Brazil

et al. 2020

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Among the diseases that affect the reproductive system of domestic animals, brucellosis in the sheep species is important because it generates significant economic losses to sheep rearing. Thus, it is a threat to the growth and productivity of sheep herds. In the face of this problem, the objective of the present research was to identify the prevalence of ovine brucellosis in herds in municipalities of the Teresina, Piauí, Brazil microregion by using the agar gel immunodiffusion assay (AGID) and indirect enzyme-linked immunosorbent assay (ELISA) serological tests. Fourteen municipalities were included in the research. Blood samples were collected from 521 pubescent animals by puncturing the jugular vein. After collection, the samples were submitted to the serological techniques, AGID and indirect ELISA, to detect anti B. ovis antibody. Of the 521 samples submitted to the AGIDtest, 23 (4.41%) were sera reagent and 498 (95.58%) negative. The indirect ELISA tests, 24 (4.61%) suspect samples and 497 (95.39%) negative samples were obtained, and there were no reagent animals in this test, only suspect. The seroprevalence of ovine brucellosis in the Teresina, Piauí microregion was 4.41%. Thus, it is possible to identify sheep with reagent serology to infection by B. ovis, that is present in municipalities in the state of Piauí, Brazil. Furthermore, AGIDwas shown to be more sensitive in detecting animals that had had contact with the etiological agent of the disease.

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Rapid serum agglutination and agar gel immunodiffusion tests associated to clinical signs in rams experimentally infected with Brucella ovis

Cited by 4 publications

References 14 publications

Indirect ELISA for diagnosis of Brucella ovis infection in rams

Indirect ELISA for diagnosis of Brucella ovis infection in rams

Serologic and molecular evidence of Brucella ovis infection in ovine and caprine flocks in the State of Minas Gerais, Brazil

Seroprevalence of ovine brucellosis in the microregion of Teresina, Piauí, Brazil

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