The transfer of antirabies immunoglobulins in cows that were prime vaccinated and cows that were revaccinated against rabies correlated to the serum titers in their offspring was evaluated. The results demonstrated that revaccination against rabies during pregnancy induces neutralizing antibody titers at a protective level that are transferred directly to calves through colostrum and reinforce the importance of revaccination for improved colostral antibody transfer and offspring protection against rabies.According to the Panamerican Health Organization (16), 2,797 cases of rabies in cattle were reported in 2004 in the Americas, which is an 87% increase over 2002 levels. Of these, 2,591 (92.6%) occurred in Latin America and 130 (4.6%) in North America. In Latin America, Brazil had 60.6% of the cases, followed by 13% in Mexico and 8% in the Andes. More recent data demonstrated that Brazil still reports more cases of rabies in domestic animals than other countries of South America, with a predominance of rabies in cattle (24).
Communication [Comunicação] Adaptation and evaluation of polymerase chain reaction for Brucella ovis detection in semen, urine and organs of rams experimentally infected[Adaptação e avaliação da reação em cadeia da polimerase para detecção de Brucella ovis em sêmen, urina e órgãos de carneiros infectados experimentalmente]
B. ovis pathogenicity was evaluated in experimentally inoculated and naturally infected rams. Ten animals were submitted to simultaneous conjunctival and intrapreputial inoculation with 2x10 9 CFU/ mL of B. ovis REO 198. After that, animals underwent physical examination and blood samples were collected for serology every week. Positive serology results started to be observed in the 3 rd week, with fluctuations in titers. Clinical changes began in the 5 th week after inoculation and were associated with positive serology in the acute phase of the disease. Presence of B. ovis in semen and urine culture was intermittent. Three non-inoculated animals showed natural infection. B. ovis was shed twice in semen of one serology-negative animal. The study underscored the pathogenic characteristics of B. ovis REO 198 in Santa Inês rams, as well as the importance of animals as potential sources of infection. Key words: B. ovis, brucellosis, ovine, clinical signs, experimental inoculation
ResumoPatogenicidade da B. ovis foi avaliada em carneiros inoculados experimentalmente e naturalmente infectados. Dez animais foram submetidos à inoculação conjuntival e intrapreputial simultânea com 2x10 9 UFC/mL de B. ovis REO 198. Depois disso, os animais foram submetidos ao exame físico e amostras de sangue foram coletadas para sorologia a cada semana. Sorologia positiva começou a ser observada na terceira semana, com flutuações nas titulações. As alterações clínicas começaram na quinta semana após a inoculação e foram associadas com sorologia positiva na fase aguda da doença. A presença de B. ovis em sêmen e cultura de urina foi intermitente. Três animais não inoculados apresentaram infecção natural. B. ovis foi eliminada duas vezes em sêmen de animal com sorologia negativa. O estudo destacou as características patogênicas de B. ovis REO 198 em carneiros Santa Inês, bem como a importância dos animais como potenciais fontes de infecção.
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