Embriogênese somática a partir de calos de cultivares de laranja doce

Castro, Lívia Mendes; Filho, Francisco de Assis Alves Mourão; Mendes, Beatriz Madalena Januzzi; Miyata, Luzia Yuriko

doi:10.1590/s0103-84782010000800026

Cited by 3 publications

(2 citation statements)

References 11 publications

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“…ME stimulates the activity of hydrolytic enzymes (Kim et al 2010) that facilitate the breakdown of starch into maltose (Jung et al 2011). Maltose is the best carbohydrate source for the induction of somatic embryogenesis in Citrus sinensis L. Osbeck at concentrations of 37 and 75 mM (Castro et al 2010). Inositol, most abundantly found in the form of MI, cis-1,2,3,4-trans-4,6cyclohexanehexol is a cyclic polyalcohol with an important role as a structural base for second messenger in eukaryotic cells such as inositol phosphates (IPn).…”

Section: Introductionmentioning

confidence: 99%

The effects of an osmoregulator, carbohydrates and polyol on maturation and germination of ‘Golden THB’ papaya somatic embryos

Chagas

Cipriano

Lopes

et al. 2018

An. Acad. Bras. Ciênc.

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This study evaluated the effect of osmoregulators and carbohydrates on the maturation and germination of somatic embryos of papaya 'Golden THB'. Cotyledon explants from papaya seedlings germinated in vitro on basal MS medium were cultured on somatic embryogenesis induction medium (IM) containing MS salts, myo-inositol, sucrose, agar and p-chlorophenoxyacetic acid. After 50 days, embryogenic calli were transferred onto maturation media (MM) for 45 additional days. For experiment 1, a MS-based medium supplemented with abscisic acid, activated charcoal and concentrations of PEG 6000 (0; 40; 50; 60 and 70 g L-1) was used, whereas for experiment 2 malt extract concentrations (0; 0.1; 0.2; 0.3 and 0.4 g L-1) were assessed. The normal cotyledonary somatic embryos produced in experiment 2 were transferred to the germination medium (GM). The GM consisted of full-strength MS medium, sucrose, agar and was supplemented with myo-inositol at varying concentrations (0; 0.275; 0.55 and 0.825 mM). The PEG concentrations tested impaired the maturation of 'Golden THB' papaya somatic embryos. The MM, supplemented with malt extract at 0.153 g L-1 , promoted the greatest development of normal somatic embryos (18.28 SE calli-1), that is, two cotyledonary leaves produced 36.56 SE calli-1. The supplementation with 0.45 mM myo-inositol provided the highest germination percentage (47.42%) and conversion to emblings.

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Section: Introductionmentioning

confidence: 99%

The effects of an osmoregulator, carbohydrates and polyol on maturation and germination of ‘Golden THB’ papaya somatic embryos

Chagas

Cipriano

Lopes

et al. 2018

An. Acad. Bras. Ciênc.

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“…Fotos: Juliana Degenhardt-Goldbach Castro et al (2010), na indução da embriogênese somática em tecidos de laranja doce, concluíram que a glicose não foi eficiente na formação de calos embriogênicos, sendo as melhores fontes de carboidratos a maltose, seguida pela lactose, nas concentrações de 37 e 75 mM. Embriogênese somática não foi observada nos meios de cultura contendo galactose, glicose ou sorbitol para nenhuma cultivar estudada.…”

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Efeito da fonte de carbono na embriogênese somática em Bactris gasipaes

et al. 2016

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A metodologia de camada fina de células foi utilizada com sucesso para indução de embriogênese somática em região do meristema apical de plantas de pupunha mantidas em casa-de-vegetação. O efeito de três fontes de carbono: sacarose, glicose e manitol na indução de embriogênese somática foi avaliado. Embriões somáticos foram observados após a indução apenas nos meios contendo sacarose ou glicose em igual proporção.Effect of carbon source on somatic embryogenesis of Bactris gasipaesThin cell layer of meristematic region was successfully used to induce somatic embryogenesis in greenhouse-grown plants of pejibaye. The effects of three sources of carbohydrates, sucrose, glucose and mannitol on induction of somatic embryogenic calli were evaluated. Somatic embryos were observed on media containing either sucrose or glucose.Index terms: Thin cell layer, Glucose, Sucrose

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