The objective of this study was to evaluate the effect of pure glucose, glucose plus fructose, and fructose on the blood glucose of omnivorous fi sh tambaqui (Colossoma macropomum), Nile tilapia (Oreochromis niloticus), piau (Leporinus elongatus), and carnivorous fi sh hybrid Amazon catfi sh (Pseudoplatystoma fasciatum × Leiarius marmoratus), pacamã (Lophiosilurus alexandri), and traíra (Hoplias malabaricus). In each species, the dose 1 mL per fi sh with 1,000 mg kg of body weight -1 of glucose, fructose or glucose plus fructose were tested intraperitoneally. Blood glucose was measured at times 0 (control), 0.5, 1, 2, 4, 8, 16, and 24 h. The administration of 1,000 mg of glucose or glucose plus fructose per kg of live weight causes hyperglycemia in the omnivorous and carnivorous species studied. In the omnivorous species, glycemic levels were reduced from 2 to 4 h, and the regulation to baseline occurred from 4 to 8 h. In the carnivores fi sh, blood glucose levels declined between 1 and 8 h, and return to baseline was observed from 8 to 16 h. Tambaqui was also intolerant to high concentrations of fructose. Blood glucose levels are regulated in a shorter time in Nile tilapia (mainly), piau and pacamã.