Efeito do tipo de ramo e do regime de luz fornecido à planta matriz no estabelecimento in vitro de araçazeiro cv. "Irapuã"

Souza, Joseane Almeida de; Schuch, Márcia Wulff; Silva, Luciane Couto da

doi:10.1590/s0103-84782006000600041

Cited by 7 publications

(7 citation statements)

References 2 publications

(1 reference statement)

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“…These authors had problems with bacterial contamination (average of 50%, with 95% for the control group) and fungal contamination (average of 13.34%, with 55% for the control group). Similar data was observed by Souza, Schuch, and Silva (2006) who, when testing the introduction of P. cattleianum cv. "Irapuã" using semi-lignified and herbaceous shoots, found that the average percentage of bacterial and fungal contaminations were 89.68% and 46.85%, respectively.…”

Section: In Vitro Establishment Of Red Araçá Seedlingssupporting

confidence: 84%

Micropropagation’s Complete Protocol of Red Araçá (Psidium cattleianum, Myrtaceae) from Germinated Seeds in vitro

Freire¹,

Gardin²,

Baratto³

et al. 2018

JAS

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Red Araçá's (Psidium cattleianum) micropropagation processes have shown enormous potential both in terms of research and as a sustainable native resource to be used in the areas of food production, ecology, and pharmacology. Currently, however, despite that potential, research efforts involving this myrtaceae, native to the Brazilian Atlantic Forest, have been scarce. With that in mind, this study set out to establish micropropagation techniques that would allow the development of a feasible protocol to be used with Red Araçá, achieving its mircropropagation from in vitro germinated seeds. Different types of explants were tested for in vitro establishment. For the multiplication of nodal segments, different concentrations of BAP and IAA combinations were tested in an MS medium. Using the same medium, different concentrations of ampicillin were applied in order to determine its influence on the decontamination of the apical segments. The BAP and IAA combinations were also used to test their effects on the in vitro explants' development and rooting. During pre-acclimatization, survival of in vitro rooted plants was tested in a nebulizer chamber, using a commercial substrate and that same substrate mixed with washed sand (1:1). In essence, it was indeed possible to develop a complete protocol for the micropropagation of the Red Araçá from seedlings obtained by in vitro germination. The in vitro introduction of the Red Araçá was rather efficient, independently of the type of explants used. As the BAP and IAA concentrations increased, so did the in vitro seedlings' development (7 leaves explant -1 ) and rooting (67%). Additionally, the in vitro rooted plants exhibited a high rate of survival (80%) in the pre-acclimatization phase, independently of the substrate used.

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Section: In Vitro Establishment Of Red Araçá Seedlingssupporting

confidence: 84%

Micropropagation’s Complete Protocol of Red Araçá (Psidium cattleianum, Myrtaceae) from Germinated Seeds in vitro

Freire¹,

Gardin²,

Baratto³

et al. 2018

JAS

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“…A utilização de técnicas de cultura de tecidos com o objetivo de melhorar a rentabilidade das culturas tem-se apresentado como instrumento importante que pode ser explorado pelos pesquisadores, produzindo plantas com elevada qualidade sanitária (Souza et al, 2006).…”

Section: Introductionunclassified

Otimização de um protocolo para micropropagação da oliveira Ascolano 315

Villa

Pasqual²,

Freitas³

2010

Rev. Ceres

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Optimization of a protocol for the micropropagation of olive tree cv. Ascolano 315Micropropagation can be a viable technique for the multiplication of olive trees. The objective of this work was to induce multiplication in explants of olive tree. Nodal segments with 2 cm length, without leaves, derived from in vitro plantlets of cultivar Ascolano 315 were excised and inoculated in test tubes. The tubes contained 15 mL of OM (Olive medium) culture medium supplemented with 2 g L -1 of activated charcoal, 4 concentrations of 6-benzilaminopurin (BAP) and 4 concentrations of coconut water and solidified with 5.5 g L -1 of agar. The pH was adjusted to 5.8 before medium sterilization at 121ºC and 1 atm for 20 min. The experimental design was complete randomized in a 4 x 4 factorial scheme. The explants were kept in a growth room 25±1ºC, light intensity of 32 ì mol m -2 s -1 and photoperiod of 16 hours, for 70 days. The culture medium OM added of 1.0 mg L -1 of BAP and 100 mL L -1 of coconut water provided the greatest length and biomass of the aerial part. The largest number of roots was obtained with 0.5 mg L -1 of BAP associated with 25 mL L -1 of coconut water.

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“…In evaluating the effect of a light regime on the mother plant and the branch type used for the in vitro establishment of araça (Psidium spp. ), [13] observed lower rates of contamination when using nodal segment explants of herbaceous branches, and obtained the highest survival rates when the mother plants were kept in darkness. These results are likely due to the reduced exposure of younger branches to contamination in the environment from which the explants were harvested.…”

Section: In Vitro Establishment and Micropropagationmentioning

confidence: 89%