Estabelecimento de pereira (Pyrus spp.) in vitro a partir de meristemas e gemas

Erig, Alan Cristiano; Fortes, Gérson Renan de Luces

doi:10.1590/s0103-84782002000400005

Cited by 6 publications

(2 citation statements)

References 6 publications

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“…In addition, plants stress may have occurred, making them more fragile and prone to a contaminants attack. The result found for this variable is in contrast with the percentages obtained by Erig and Fortes (2002) when evaluating pear tree (Pyrus spp,) cultivars and different explants collection periods to isolate in vitro meristems. They observed greater explants contamination by bacteria in summer for the three cultivars (Garber, Smith and Carrick -17.7%, 15.5% and 5.1%).…”

Section: Resultscontrasting

confidence: 78%

Collection periods in the in vitro establishment of raspberry tree cultivars

Fagundes

Moreira

Timm

et al. 2017

ASB J.

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The raspberry tree, a species from the Rosaceae family and the Rubus genus, is included in the group of small fruit that has been drawing the attention of the global consuming market for carrying antioxidant properties and substances capable of fighting free radicals, bringing health benefits. The objective of this work was to evaluate the effect of collection periods on the in vitro establishment of raspberry trees (Rubus idaeus L.). Explants were collected in different seasons of the year (winter, spring, summer and autumn), and were inoculated in a MS culture medium. The experimental design was totally randomized, and arranged in a factorial scheme. At 28 days, the following variables were evaluated: oxidation, fungal and bacterial contamination survival and establishment. There was less phenolic oxidation in raspberry tree explants collected in winter. Explants contamination by endogenic bacteria occurred in higher percentages in winter. Although summer was the season of the year with higher percentage of explants’ phenolic oxidation for both cultivars, the highest in vitro establishment percentages for the Heritage cultivar occurred in summer and fall. However, for the Fallgold cultivar, spring and winter were the seasons with greater in vitroestablishment.

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Section: Resultscontrasting

confidence: 78%

Collection periods in the in vitro establishment of raspberry tree cultivars

Fagundes

Moreira

Timm

et al. 2017

ASB J.

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“…), obtiveram contaminação bacteriana em 45,7% das gemas e 18,8% dos meristemas, utilizando-se como rotina de assepsia somente álcool a 70% e hipoclorito de sódio FORTES, 2002).…”

Section: Resultsunclassified

Desinfestação e estabelecimento in vitro de explantes de bananeira 'Grande Naine' em diferentes concentrações de hipoclorito de sódio

2011

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RESUMO -A maioria dos plantios de bananeira ainda é realizada utilizando mudas tradicionais, mas outros métodos de propagação, como a micropropagação in vitro, vêm sendo desenvolvidos e aperfeiçoados, para elevar a taxa de multiplicação em curto espaço de tempo e melhorar a qualidade da produção de mudas. Contudo, a contaminação é um dos maiores problemas desta técnica. Este trabalho teve por objetivo avaliar a eficiência da descontaminação de explantes de bananeira com o uso de diferentes concentrações de cloro ativo durante a assepsia do explante. O delineamento experimental utilizado foi inteiramente casualizado e constituído de cinco tratamentos e cinco repetições, sendo cada repetição representada por 5 explantes em diferentes concentrações de cloro ativo, sendo: T1 (testemunha, sem cloro ativo); T2 (0,5%); T3 (1,0%); T4 (1,5%), e T5 (2%). Os dados obtidos foram submetidos à análise de variância, e as médias, comparadas pelo teste de Tukey, a 5% de probabilidade. Os resultados permitiram concluir que a maior eficiência dentre os tratamentos testados foi a imersão dos explantes em hipoclorito de sódio com 2% de cloro ativo, sendo as doses testadas não tóxicas aos explantes, permitindo o desenvolvimento normal dos mesmos, concluindo assim que essa concentração possa ser utilizada para o controle de contaminações para micropropagação de bananeira cv. Grande Naine. Termos para indexação: cultura de tecidos de plantas, cloro ativo, Musa sp. DISINFESTATION AND ESTABLISHMENT OF IN VITRO EXPLANTS OF BANANA 'GRANDE NAINE' IN DIFFERENT CONCENTRATIONS OF SODIUM HYPOCHLORITEABSTRACT -Brazil is one of the world largest producers of bananas, with a planted and harvested area of 504.586 ha in 2006 (IBGE, 2008). Most of the banana plantations are still cultivated using the traditional rooted young plants, but other methods of propagation such as in vitro micropropagation are constantly being developed and improved, to increase the multiplication rate in short time and to improve the quality of the seedlings production. However, contamination is a major problem of this technique. This study aimed to evaluate the efficiency of decontamination of banana explants, using different concentrations of active chlorine during the sterilization of the explants. The experimental design was randomized and consisted of five treatments and five repetitions, each one of 5 explants, at different concentrations of active chlorine, T1 (control) without active chlorine, T2 (0,5%), T3 (1,0%), T4 (1.5%) and T5 (2%). The contaminations were subjected to analysis of variance and means compared by Tukey test at 5% probability. The results showed that the highest efficiency among the treatments was the immersion of explants in sodium hypochlorite solution with 2% active chlorine, and the doses tested were not toxic to the explants, allowing normal development of them, thus concluding that concentration can be used to control contamination in banana micropropagation.

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LED Lighting Effects on Plant Growth and Quality of Pyrus communis L. Propagated In Vitro

et al. 2022

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Fluorescent lights are typically used as light source in indoor horticultural production, including micropropagation. However, light emitting diodes (LEDs) have been recently used for plant growth under controlled environment. Major advantage of LEDs is wavelength specificity, that allows to adequately adjust the spectra according to plant needs. The possibility of using LED as primary light source for the micropropagation of Pyrus communis L. has been investigated in this work. It was proceeded to the optimization of a protocol of micropropagation using a LED lamp as the primary light source inside of the growth chamber with specific wavelengths, to reduce energy consumption and increase the quality of the micro propagated plants. Explants were maintained in a growth chamber and exposed to three different continuous spectrum LED lamps (AP67, NS1, G2) as a primary light source and fluorescent lamps (control) for 4 weeks. At the end of four weeks period, it was proceeded to the morphometric and biochemical analysis. Shoot and leaf growths were more influenced by LED lamps as compared to fluorescent lamps (control) in both cultivars. The results showed that the shoots of William and San Giovanni cultivars showed significant differences in morphological and physiological traits, as well as in chlorophyll, carotenoid, and MDA contents. Highest number of neo-formed shoots and nodes were observed in the plantlets of cv William under AP67 LED followed by NS1 and G2 LED lights respectively as compared to the white light (control), whereas same trend was observed in cv San Giovanni under AP67 LED, but it showed higher shoots and node numbers under control LED lamps as compared to both NS1 and G2. The photosynthetic pigments were significantly decreased in leaves of both cultivars when grown under LEDs as compared to the control fluorescent lamps. Moreover, the AP67 LED light had also significant effects on the protein and MDA contents in the leaves of both cultivars in comparison to all other treatments. This work underlines the importance of the modulation of light sources in relation with different species and varieties, allowing optimizing the proliferation phase and the efficiency of Pyrus communis L. micropropagation protocol. Moreover, this protocol can be improved with further studies to examine the response of the plantlets to the ex-vitro acclimatization because the possibility of using LED light for the micropropagation of pear can be considered as a valuable alternative for its ecologically sustainable production.

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Estabelecimento de pereira (Pyrus spp.) in vitro a partir de meristemas e gemas

Cited by 6 publications

References 6 publications

Collection periods in the in vitro establishment of raspberry tree cultivars

Collection periods in the in vitro establishment of raspberry tree cultivars

Desinfestação e estabelecimento in vitro de explantes de bananeira 'Grande Naine' em diferentes concentrações de hipoclorito de sódio

LED Lighting Effects on Plant Growth and Quality of Pyrus communis L. Propagated In Vitro

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