Development of a potentiometric flufenamate ISE and its application to pharmaceutical and clinical analyses

Abstract

As características, o desempenho e a aplicação de um eletrodo do tipo Pt Hg Hg 2 (FF) 2 Grafite, FF=íon flufenamato, são descritas. O eletrodo responde a FF com sensibilidade de (-58,6 1,2) mV década -1 no intervalo de 1,0 10 -6 -1,0 10 -2 mol L -1 , a pH 6,0 -9,0 e com um limite de detecção de 7,1 10 -7 mol L -1 . O eletrodo é de custo relativamente baixo e facilmente construído, apresenta um rápido tempo de resposta (10-25 s) e pode ser usado por um período de 5 meses sem qualquer variação considerável nas suas características de desempenho. O eletrodo proposto mostrou boa seletividade para o íon flufenamato na presença de várias substâncias, bem como na presença de alguns carboxilatos e ânions inorgânicos. O eletrodo foi aplicado com sucesso na determinação de ácido flufenâmico em medicamentos e amostras de soro humano.The characteristics, performance, and application of an electrode, namely, Pt Hg Hg 2 (FF) 2 Graphite, where FF stands for flufenamate ion, are described. This electrode responds to FF with sensitivity of (-58.6 1.2) mV decade -1 over the range 1.0 10 -6 -1.0 10 -2 mol L -1 at pH 6.0 -9.0 and a detection limit of 7.1 10 -7 mol L -1. The electrode is easily constructed at a relatively low cost with fast response time (within 10-25 s) and can be used for a period of 5 months without any considerable change in its performance characteristics. The proposed electrode displayed good selectivity for flufenamate in the presence of several substances as well as in the presence of some carboxylate and inorganic anions. The electrode was successfully applied to the determination of flufenamic acid in pharmaceuticals and human serum samples.Keywords: flufenamate-sensitive electrode, potentiometry, dosage forms, blood serum IntroductionFlufenamic acid (FFA), [N-( , , -trifluoro-m-tolyl) anthranilic acid] is an analgesic, anti-inflammatory and antipyretic drug. It is extensively used in the treatment of arthritis and other illnesses related to muscular-skeletal problems.1 However the use of this drug has been implicated in some cases of nephrotoxicity.2 Due to the importance of the assay of FFA for pharmaceutical formulations and biological fluids, several analytical methods have been developed for the quantitative determination of this drug in both pharmaceutical and biological samples.These methods include spectrophotometry, 21,22 However, many of these methods are often time-consuming, technically demanding and require the use of costly, highly specialized instruments. Thus, there is an important demand for simple, low-cost, sensitive and rapid alternative methods for the determination of FFA in pharmaceuticals and biological fluids.Potentiometric methods with ion-selective electrodes (ISE's) have proved to be effective for the analysis of pharmaceutical formulations and biological samples, because these sensors offer the advantages of simple design, construction, and manipulation, reasonable selectivity, fast response time, applicability to colored and turbid solutions and possible interfacing with au...

Electroanalytical Determination of Doripenem using a Screen-Printed Electrode

“…DP, similar to other carbapenems, was developed for the treatment of hospitalised patients with moderate or severe bacterial infections (Jones et al, 2004) Doripenem has been determined in its pharmaceutical formulation and in plasma using different techniques, including spectrophotometry (Piontek and Ska, 2010) chromatography High Performance Liquid Chromatography (HPLC) with various detection methods (Sutherland and Nicolau, 2007;Ikeda et al, 2008) and liquid chromatography (Dailly et al, 2011;Ohmori et al, 2011). The development and application of ion-selective electrodes for pharmaceutical analysis continue to be of interest because these sensors offer the advantage of simple design and operation, reasonable selectivity, fast response, applicability to coloured and turbid solutions and possible interface with automated and computerised systems (Santini et al, 2008). For these advantages, ISEs have found various applications: in clinical chemistry, environmental protection, water, soil and analytical chemistry in general (Kormosh et al, 2008).…”

A New Approach for Decreasing the Detection Limit of Gentamicin Ion‐selective Electrodes by Incorporation of Multiwall Carbon Nanotubes (MWCNTs)/Lipophilic Anionic Additives

“…In view of distinct advantage of ISEs huge amount of efforts have been devoted to make selective sensors for different ions [3]. The development and application of ion-selective electrodes for pharmaceutical analysis continue to be of interest because these sensors offer the advantage of simple design and operation, reasonable selectivity, wide dynamic range, low cost, fast response, applicability to colored and turbid solutions and possible interface with automated and computerized systems [4,5]. For these advantages, ISEs found various applications in clinical chemistry, in environmental protection, in agricultural, in foodstuff samples and in analytical chemistry [6].…”

A New Potentiometric Sensor for Determination of Pethidine hydrochloride in Ampoules and Urine

“…Potentiometric methods with ion-selective electrodes (ISE's) have proved to be effective for the analysis of pharmaceutical formulations and biological samples, because these sensors offer the advantages of simple design, construction, and manipulation, reasonable selectivity, fast response time, applicability to colored and turbid solutions and possible interfacing with automated and computerized systems. [15][16][17]. A number of ISEs based on PVC membrane [10][11][12][13][14], for determination of pethidine have been reported.…”

“…The advantages of carbon paste electrodes, such as che- mical inertness, robustness, low cost, renewability, very low background current, stable response, low ohmic resistance and no need for internal solution over conventional polymeric membrane electrodes has attracted the attention of researchers in recent years [15][16][17][18]. After careful review of literature, there is no report on determination of pethidine hydrochloride using a carbon paste electrode.…”