Effects of aroeira (Schinus terebinthifoliu Raddi) oil on cutaneous wound healing in rats

Abstract

PURPOSE:To evaluate the effects of aroeira (Schinus terebinthifolius) ointment on skin wound healing in rats. METHODS:Adult male rats (n=20) were divided into four groups of five animals each, as follows: G4, G7, G14 and G21, which corresponds to 4 th , 7 th , 14 th and 21 th days postoperatively. Each animal were made two incisions on the skin, including the subcutaneous tissue, in the right and left sides of thoracic region, separated by a distance of two inches. The right lesion was treated with base ointment (vaseline, lanolin); the left one was treated with base ointment containing 5% of aroeira oil. At the end of each experimental period the lesions were evaluated for the contraction degree. Then held the collection of fragments that were fixed in 10% formalin and processed for paraffin embedding. In the histological sections (5μm) was evaluated the morphology and quantified the collagen and blood vessels.The data obtained were submitted to ANOVA test complemented by Tukey-Kramer test (p<0.05). RESULTS:The contraction of the lesions was higher in wounds treated with aroeira oil than in controls at 7 th and 14 th days (p<0.01), whereas in the 21 st day all lesions were already completely healed. The morphology showed granulation tissue more developed, with fibroblasts more bulky and collagen fibers more arranged in the experimental group at 4 th , 7 th and 14 th days. The morphometry showed a significant increase in the quantification of collagen fibers in the experimental group at 7 th and 14 th days (p<0.05). CONCLUSION:The aroeira oil accelerates the healing process of wounds as a macroscopic, morphological and morphometrical analysis.

Schinus terebinthifolius Raddi ( Aroeira) leaves oil attenuates inflammatory responses in cutaneous wound healing in mice

“…This experimental model has been used to study the biological mechanisms involved skin wound healing 1,2 . STRO was applied topically as natural compounds with healing potential can be tested directly in wound bed 18 .…”

“…The area of wounds was measured with a digital caliper, on days 0, 3, 7 e 14 after surgery and the results were expressed as percentage closure, relative to original size (wound contraction = original wound area -present wound area / total wound area x 100) 1 . No signs of infection were detected in the wounds area.…”

“…The images were digitized with Olympus BX43 microscope with an Olympus Q-Color 5 micro camera. Angiogenesis were determined by counting blood vessels in the entire area of wound tissue on H&E stained sections, cross sections images were obtained from 6 sequential fields from each wound and observed with an objective (x40) in light microscopy (final magnification = x400) 1 .…”

“…It has been used in folk medicine as teas, infusions or tinctures as an anti-inflammatory, febrifuge, analgesic, and depurative agent; also, it has been used to treat skin wounds, repair injury in respiratory, digestive and genitourinary systems [1][2][3][4][5][6] . Previous reports have demonstrated that S. terebinthifolius extracts or fractions is rich in polyphenols, which display antioxidant, antibacterial, antifungal and anti-allergic activities in different experimental models [7][8] .…”

Mast cell concentration and skin wound contraction in rats treated with Brazilian pepper essential oil (Schinus terebinthifolius Raddi)

“…However Estevão et al 17 and Lipinski et al 18 show the aroeira oil had a beneficial effect on wound healing by second intention.…”