Preconditioning with L-alanyl-glutamine reduces hepatic ischemia-reperfusion injury in rats

Júnior, Raimundo José Cunha Araújo; Júnior, Raimundo Gerônimo da Silva; Vasconcelos, Marcelo Pinho Pessoa de; Guimarães, Sérgio Botelho; Vasconcelos, Paulo Roberto Leitão de; Garcia, José Huygens Parente

doi:10.1590/s0102-86502011000700003

Cited by 10 publications

(9 citation statements)

References 19 publications

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“…It does not possess an antioxidant effect on its own. However, it has been shown to protect cells and tissues against a variety of stressful stimuli including inflammation [7,8,9], ischemia [10,11,12], infection, fibrosis and cancer [13,14,15,16]. …”

Section: Introductionmentioning

confidence: 99%

“…In recent years, the protective effects of Gln against I/R-induced organ injury have attracted more and more attention in the scientific field [10,11,12]. For rodents, some reports have demonstrated that pretreatment with Gln helps to reduce I/R injury in a variety of organs by using different I/R animal models [11,17,18,19,20].…”

Section: Introductionmentioning

confidence: 99%

“…For rodents, some reports have demonstrated that pretreatment with Gln helps to reduce I/R injury in a variety of organs by using different I/R animal models [11,17,18,19,20]. Nevertheless, few studies have been carried out on this topic using the obstructive jaundice hepatic I/R model.…”

Section: Introductionmentioning

confidence: 99%

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Preconditioning with Glutamine Protects against Ischemia/Reperfusion-Induced Hepatic Injury in Rats with Obstructive Jaundice

Dai

Peng

et al. 2014

Pharmacology

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Objective: To ascertain whether glutamine (Gln) pretreatment protects rats with obstructive jaundice from hepatic ischemia/reperfusion (I/R) injury and to determine the underlying molecular mechanisms. Methods: An obstructive jaundice rat model was developed by bile duct ligation. On the first day after the operation, all rats were randomized into two groups and received oral Gln or normal saline (NS) daily for 7 days. Then both groups underwent a 15-min liver ischemia via the Pringle maneuver. Blood samples as well as liver and intestinal tissues were harvested and measured after 1, 6 and 24 h of reperfusion. Results: The results showed that the histological morphology of the liver and intestinal tissues significantly improved in the Gln group after I/R injury compared with the NS group. Serum proteins and enzymes associated with hepatic function also significantly improved in the Gln group. The level of glutathione increased and the levels of malondialdehyde and myeloperoxidase decreased in the Gln group. The levels of interleukin-1β and tumor necrosis factor-α decreased in the Gln group. Moreover, bcl-2 protein expression was upregulated and intercellular adhesion molecule 1 and bax protein expression downregulated in the Gln group; the caspase 3 mRNA level significantly increased in the Gln group. Conclusions: The study demonstrates that preconditioning with Gln significantly improves hepatic structure and function after I/R injury in rats with obstructive jaundice. The protective effect of Gln was mediated by the inhibition of reactive oxygen species and inflammation as well as a reduction in hepatocyte apoptosis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Preconditioning with Glutamine Protects against Ischemia/Reperfusion-Induced Hepatic Injury in Rats with Obstructive Jaundice

Dai

Peng

et al. 2014

Pharmacology

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“…L-Ala-Gln has been used to protect the rat testis from I/R lesion inducing a decrease in lipid peroxidation and oxidative stress 2 . This glutamine dipeptide has also been able to promote hepatic protection from liver normothermic I/R in the rat 3 . The intravenous use of 50g of this dipeptide over three hours preceeding limb revascularization has attenuated muscular tissue damage and protected from oxidative stress in patients with critical ischemia of inferior members 4 .…”

Section: Introductionmentioning

confidence: 99%

Preconditioning with L-alanyl-glutamine upon cerebral edema and hypocampus red neurons counting in rats subjected to brain ischemia /reperfusion injury

Vasconcelos

Guimarães²,

Campelo³

et al. 2015

Acta Cir. Bras.

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Conception, design, intellectual and scientific content of the study, critical revision, final approval. ABSTRACT PURPOSE:To evaluate the effects of the dipeptide L-alanyl-glutamine (L-Ala-Gln) as a preconditioning agent to potentially promote reduction in the intensity of lesion or induction of resilience in rats subjected to global cerebral ischemia/reperfusion (I/R) injury. METHODS:Thirty-six male Wistar rats weighing 280-300g were randomly assigned to six groups (n=6). Groups Sham 1h and 24hwere treated with saline and spared of further interventions. The remaining groups were submitted to clamping of the common carotid arteries for 30 minutes (ischemia) and treated with saline (SS) or L-Ala-Gln. Brain reperfusion was allowed for 1or 24 h. L-Ala-Gln was administered intravenously (0.75g/kg) 30 minutes before sham procedure or induction of global brain I/R injury. Brain edema and red neuron counting were determined. Results were expressed as Mean±SD for normal results and Median±Percentile for non parametric data. Significance was established at p<0.05. RESULTS:Global I/R injury promoted an increase in brain edema at 24 h after reperfusion, whereas preconditioning with L-Ala-Gln induced no change in edema. On the other hand, L-Ala-Gln preconditioning decreased significantly red neurons counting both at 1h and 24h post reperfusion (p<0.05). CONCLUSION:There was a significant preconditioning effect with L-Ala-Gln decreasing cell death (red neurons counting) at early (1h) and late reperfusion (24h) in the cerebral tissue.

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“…Many experimental I/R hepatic studies verified important aspects, such as the necrosis which affects extensive areas of parenchymal cells consistent with the massive release of alanine transaminase (ALT) in serum, indicate that necrosis is the main cause of liver injury during I/R [9][10][11] . Other studies that analyzed proapoptotic factors, displayed a downregulation of the Bax and cleaved Caspase-3 expression, when associated with the ischemic perconditioning, having less tissue damage 12 .…”

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confidence: 96%

Perconditioning associated to hypertonic saline solution on liver function improvement after ischemia/reperfusion injury

et al. 2017

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Preconditioning with L-alanyl-glutamine reduces hepatic ischemia-reperfusion injury in rats

Abstract: L-Ala-Gln preconditioning in rats submitted to hepatic I/R significantly reduces ALT, LDH and Caspase-3 expression, suggesting hepatic protection.

Cited by 10 publications

References 19 publications

Preconditioning with Glutamine Protects against Ischemia/Reperfusion-Induced Hepatic Injury in Rats with Obstructive Jaundice

Preconditioning with Glutamine Protects against Ischemia/Reperfusion-Induced Hepatic Injury in Rats with Obstructive Jaundice

Preconditioning with L-alanyl-glutamine upon cerebral edema and hypocampus red neurons counting in rats subjected to brain ischemia /reperfusion injury

Perconditioning associated to hypertonic saline solution on liver function improvement after ischemia/reperfusion injury

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