Animal model of chronic abdominal hernia in rabbit

Silva, Walter da; Fagundes, Djalma José; Seidel, Amélia Cristina; Taha, Murched Omar

doi:10.1590/s0102-86502009000400003

Cited by 3 publications

(6 citation statements)

References 34 publications

(57 reference statements)

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“…Almost all the studies with a few exceptions were on wound healing as well as scar formation and they were sourced on the promotion of an acute full-depth defect in the abdominal wall that was patched up by a mesh and/or a surgical procedure [32][33][34][35]. Previous research suggests that a full-depth defect in a rabbit model was practicable and dependable to support an incisional hernia that could reproduce an appropriate pathophysiological state and could be used as a standard model to enhance the assessment of approaches to hernia repair [27]. A histological study showed that the 3D printed PP mesh worked well with considerable fibro vascular tissue in growth and minor inflammation (Figure 6 A).…”

Section: In Vivo Evaluationmentioning

confidence: 99%

Personalized 3D printed ciprofloxacin impregnated meshes for the management of hernia

Qamar

Abbas

Irfan

et al. 2019

Journal of Drug Delivery Science and Technology

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This study aims to explore the application of fused deposition modeling (FDM), a widely used 3D printing technique, in the fabrication of personalised hernial meshes. Eight different meshes with and without the loading of an antibiotic (ciprofloxacin HCl) were designed using two different polymers, polypropylene (PP) and polyvinyl alcohol (PVA), having different pore size, shape and thread thickness. Printed meshes were evaluated for their mechanical, drug loading and release characteristics. Among the fabricated meshes, polypropylene has shown adequate printability characteristics where polyvinyl alcohol filaments showed comparatively easy handling during 3D printing. All the printed meshes showed satisfactory mechanical properties. However, the PVA meshes showed slightly faster release as compared to PP based meshes. Moreover, in-vivo testing in rabbit models was also performed for assessing biocompatibility and adhesiogenecity. Post-implantation observations in animal models revealed no signs of implant rejection and the extent of adhesions to the visceral tissue was mild to moderate. Animals implanted with ciprofloxacin HCl loaded meshes exhibited fewer fluctuations in body temperature, and they had faster-wound healing capacity. This work demonstrated for the first time that FDM is an effective and low-cost alternative for the manufacturing of tailored mesh for the management of hernia. The method has also been successfully employed for the preparation of drug loaded 3D mesh, which may be effectively used against post-surgical infections.

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Section: In Vivo Evaluationmentioning

confidence: 99%

Personalized 3D printed ciprofloxacin impregnated meshes for the management of hernia

Qamar

Abbas

Irfan

et al. 2019

Journal of Drug Delivery Science and Technology

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“…However, there have been few reports on a comparison of HADM and porcine acellular dermal matrices (PADM) in hernia repair (Stanwix et al 2010 ). The purpose of this study was to compare the effectiveness of HADM and PADM in a rabbit incisional hernia repair model (Carvalho et al 2001 ; LeBlanc et al 2002 ; Judge et al 2007 ; Da Silva et al 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of human acellular dermis versus porcine acellular dermis in an in vivo model for incisional hernia repair

et al. 2011

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Incisional hernias commonly occur following abdominal wall surgery. Human acellular dermal matrices (HADM) are widely used in abdominal wall defect repair. Xenograft acellular dermal matrices, particularly those made from porcine tissues (PADM), have recently experienced increased usage. The purpose of this study was to compare the effectiveness of HADM and PADM in the repair of incisional abdominal wall hernias in a rabbit model. A review from earlier work of differences between human allograft acellular dermal matrices (HADM) and porcine xenograft acellular dermal matrices (PADM) demonstrated significant differences (P < 0.05) in mechanical properties: Tensile strength 15.7 MPa vs. 7.7 MPa for HADM and PADM, respectively. Cellular (fibroblast) infiltration was significantly greater for HADM vs. PADM (Armour). The HADM exhibited a more natural, less degraded collagen by electrophoresis as compared to PADM. The rabbit model surgically established an incisional hernia, which was repaired with one of the two acellular dermal matrices 3 weeks after the creation of the abdominal hernia. The animals were euthanized at 4 and 20 weeks and the wounds evaluated. Tissue ingrowth into the implant was significantly faster for the HADM as compared to PADM, 54 vs. 16% at 4 weeks, and 58 vs. 20% for HADM and PADM, respectively at 20 weeks. The original, induced hernia defect (6 cm2) was healed to a greater extent for HADM vs. PADM: 2.7 cm2 unremodeled area for PADM vs. 1.0 cm² for HADM at 20 weeks. The inherent uniformity of tissue ingrowth and remodeling over time was very different for the HADM relative to the PADM. No differences were observed at the 4-week end point. However, the 20-week data exhibited a statistically different level of variability in the remodeling rate with the mean standard deviation of 0.96 for HADM as contrasted to a mean standard deviation of 2.69 for PADM. This was significant with P < 0.05 using a one tail F test for the inherent variability of the standard deviation. No significant differences between the PADM and HADM for adhesion, inflammation, fibrous tissue or neovascularization were noted.

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“…In the last 10 years, the medical science has been noticing even more the potential of the white rabbit (Oryctolagus cuniculus) of the New Zealand lineage 1 as an experimental animal model for the research in video-surgery, general surgery, orthopedics, gynecology, vascular, craniofacial and urologic [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] . However, there are few information with a good scientific evidence, regarding the perioperative care [2][3][4][5][6][7][8][9][10][11][12][13][14][15]17 .…”

Section: Introductionmentioning

confidence: 99%

“…However, there are few information with a good scientific evidence, regarding the perioperative care [2][3][4][5][6][7][8][9][10][11][12][13][14][15]17 .…”

Section: Introductionmentioning

confidence: 99%

“…For abdominal surgical procedures through laparotomy, it was proposed in several studies different anesthetic protocols for many isolated or combined routes: inhalation, endovenous, epidural and intramuscular, depending on the effects related to the anesthetic used and the physiological and behavioral parameters related to the rabbits 2,[4][5][6][7][8][9][10][11][12][13][14][15]17,[22][23][24] .…”

Section: Introductionmentioning

confidence: 99%

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Perioperative care in an animal model for training in abdominal surgery: is it necessary a preoperative fasting?

2011

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PURPOSE:Demonstrate that the rabbit may be used in the training of surgery, in addition to present its perioperative care. METHODS:Thirty two animals, with age and weight, respectively, from 3 to 5.5 months old and 3000 to 4200 grams, were undergone different periods of pre-operative fasting, exclusive intramuscular anesthesia (ketamine+xylazine), laparotomy with total gastrectomy and total splenectomy. It was dosed the pre-operative (initial) and post-surgical (end) serum blood glucose, in addition to quantify the gastric content after the resection of the part. RESULTS:The anesthetical-surgical procedure presented a mortality rate of 3.125% (1:32) and a morbidity rate of 6.25% (2:32). It was evidenced an initial mean blood glucose = 199.4 mg/dl and the end = 326.1 mg/dl. In spite of extended fasting (minimum of 2 hours for the absolute fasting and maximum of 8.5 hours for liquids, and 20.5 hours for solids) all animals presented at the end of the surgical procedure any gastric content and a blood glucose increase. Those with fasting for liquids and solids when compared to the quantity of solid gastric content, presented a moderate negative degree of correlation. CONCLUSION:The rabbit is a good model to be used in training of surgery, with a low morbi-mortality, able to be anesthetized intramuscularly, with no need of pre-operative fasting and does not present hypoglycemia even with the extended fasting period. Keywords: Animal Experimentation. Surgery. Fasting. Blood Glucose. Rabbits. RESUMO OBJETIVO:Demonstrar que o coelho pode ser utilizado no treinamento em cirurgia, além de apresentar seus cuidados perioperatórios. MÉTODOS:Trinta e dois animais, com idade e peso respectivamente, entre 3 a 5,5 meses e 3000 a 4200 gramas, foram submetidos a variados tempos de jejum pré-operatório, anestesia intramuscular exclusiva (quetamina+xilasina), laparotomia com gastrectomia e esplenectomia totais. Dosou-se a glicemia sérica pré-operatória (inicial) e pós-cirúrgica (final), além de quantificado o conteúdo gástrico pós-gastrectomia. RESULTADOS: O procedimento anestésico-cirúrgico apresentou taxa de mortalidade de 3,125 % (1:32) e morbidade de 6,25 % (2:32). Evidenciou-se glicemia média inicial = 199,4 mg/dl e final = 326,1 mg/dl. Apesar de jejuns prolongados (mínimo de 2 horas para jejum absoluto e máximo de 8,5 horas para líquidos, e 20,5 horas para sólidos) todos os animais apresentaram no final do procedimento cirúrgico algum conteúdo gástrico e aumento da glicemia. Aqueles com jejum para líquidos e sólidos quando comparados com a quantidade de conteúdo gástrico sólido e total apresentaram grau moderadamente negativo de correlação linear. CONCLUSÃO:O coelho é um bom modelo para ser utilizado em treinamento de cirurgia, com baixa morbimortalidade, passível de ser anestesiado por via intramuscular, sem necessidade de jejum pré-operatório e ausência de hipoglicemia.

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Animal model of chronic abdominal hernia in rabbit

Cited by 3 publications

References 34 publications

Personalized 3D printed ciprofloxacin impregnated meshes for the management of hernia

Personalized 3D printed ciprofloxacin impregnated meshes for the management of hernia

Evaluation of human acellular dermis versus porcine acellular dermis in an in vivo model for incisional hernia repair

Perioperative care in an animal model for training in abdominal surgery: is it necessary a preoperative fasting?

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