Efeito de um extrato de Hipérico (Hypericum perforatum) na marcação in vitro de elementos sangüíneos com tecnécio-99m e na biodisponibilidade do radiofármaco pertecnetato de sódio em ratos Wistar

Santos-Filho, Sebastião David; Bernardo-Filho, Mário

doi:10.1590/s0102-86502005000700014

Cited by 9 publications

(8 citation statements)

References 17 publications

(17 reference statements)

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“…Vegetal extracts and synthetic drugs can generate metabolites capable to promote morphological and/or physiological modifications in treated animals. Several authors have demonstrated that the biodistribution of radiopharmaceuticals may be altered by natural and/or synthetic drugs, diets and surgery 6,8,12,13,14,15 . We have previously shown that the antiparasitic drugs glucantime and mefloquine can alter the biodistribution in vivo of 99m Tc-methylenediphosphonic acid ( 99m Tc-MDP) in Wistar rats 8,12 .…”

Section: Discussionmentioning

confidence: 99%

Effect of an extract of Aloe vera on the biodistribution of sodium pertechnetate (Na99mTcO4) in rats

et al. 2009

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PURPOSE: Aloe vera is a tropical plant popularly known in Brazil as babosa. We have investigated the effect of aqueous extract of Aloe vera on the biodistribution of Na99mTcO4 and laboratorial parameters in Wistar rats. METHODS: Twelve animals were divided into treated and control groups. In the treated group, Aloe vera was given by gavage (5mg/mL/day) during 10 days. The control group received sorbitol by the same way and period. One hour after the last dose, we injected 0.1mL of Na99mTcO4 by orbital plexus. After 60 min, all the animals were killed. Samples were harvested from the brain, liver, heart, muscle, pancreas, stomach, femur, kidneys, blood, testis and thyroid and the percentage of radioactivity (%ATI/g) was determined. Biochemical dosages were performed. RESULTS: There was a significant increase of %ATI/g in blood, femur, kidneys, liver, stomach, testis and thyroid and also in blood levels of AST and ALT. A significant decrease in levels of glucose, cholesterol, triglycerides, creatinine and urea occurred. The statistical analyses were performed by Mann-Whitney test and T-Student test (p<0.05). CONCLUSION: The aqueous extract of Aloe vera facilitated the uptake of Na99mTcO4 in organs of rats and it was responsible to a high increase of levels of AST and ALT.

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Section: Discussionmentioning

confidence: 99%

Effect of an extract of Aloe vera on the biodistribution of sodium pertechnetate (Na99mTcO4) in rats

et al. 2009

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“…This finding probably explains the low levels of cholesterol and glucose in mice infected and treated with A. vera in our study. Several authors have demonstrated that the biodistribution of radiopharmaceuticals may be altered by natural and synthetic drugs, diets and surgery (Xavier Holanda et al, 2002;Bernardo et al, 2004;Santos-Filho et al, 2005;Holanda et al, 2006;Araújo-Filho et al, 2007). In this study, there was a significant increase in the %ATI/g of the Na 99m TcO 4 in the femur of mice treated with A. vulgaris, probably induced by the extract of this plant on the hydroxyapatite crystals, or the deposition of calcium phosphate in bone.…”

Section: Discussionmentioning

confidence: 99%

Effect of medicinal plants on the parasitemia of Trypanosoma cruzi and on the biodistribution of sodium pertechnetate (Na99mTcO4)

Silva

Carvalho

Holanda

et al. 2008

Braz. arch. biol. technol.

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“…Radioactivity was determined in a gamma well counter (Clinigamma, LKB, Wallac, Finland). The Follow methodology was based in Oliveira et al (2000) and Bernardo-Filho (2005).…”

Section: Study Protocolmentioning

confidence: 99%

Influence of biflorin on the labelling of red blood cells, plasma protein, cell protein, and lymphocytes with technetium-99m: in vitro study

Aquino¹,

Amorim²,

Feliciano³

et al. 2007

Rev. bras. farmacogn.

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RESUMO: "Infl uência da bifl orina na marcação do tecnécio-99m em células vermelhas do sangue, proteínas do plasma, proteínas celulares e em linfócitos: estudos in vitro". Neste artigo relatam-se os resultados de um estudo in vitro envolvendo a infl uência da bifl orina (uma o-quinona isolada de Capraria bifl ora L. que possui uma potente atividade antimicrobiana) na marcação do Tc-99m em células vermelhas do sangue, proteínas do plasma, proteínas celulares e em linfócitos. O sangue foi coletado de ratos Wistar e incubado com várias concentrações de bifl orina, e soluções de cloreto estanoso (SnCl 2 ) adicionando-se Tc-99m. O plasma (P) e as células vermelhas do sangue (CVS) foram isolados, precipitados e centrifugados, isolando-se as frações solúveis (FS) e insolúveis (FI). A maior concentração de bifl orina (100%) é capaz de reduzir a captação do Tc-99m (%ATI) nas CVS e a fi xação na FI-P. Uma solução de 0,2 mL de linfócitos (2,5 mL; 1.0 x 10 6 células/mL), obtidos por centrifugação de sangue humano tratado com Ficoll-Hypac, foi incubada com bifl orina (0,1 mL). Soluções de cloreto estanoso e Tc-99m foram então adicionadas. Os linfócitos foram separados e o %ATI presente nessas células foi avaliado. Uma redução no %ATI (de 97,85 ± 0,99 a 88,86 ± 5) foi observada para CVS e para FI-P (73,24 ± 5,51 a 20,72 ± 6,95). Os resultados não mostraram decréscimo no %ATI para os linfócitos com bifl orina.Unitermos: Capraria bifl ora, radiofarmacêuticos, pertecnetato de sódio. ABSTRACT:In this paper we report the results of an in vitro study involving the infl uence of bifl orin (an o-quinone isolated from Capraria bifl ora L. that has potent antimicrobial activity) on the Tc-99m labeling of red blood cells, plasma protein, cells protein, and lymphocytes. Blood was withdrawn from Wistar rats and incubated with various concentrations of bifl orin, and solutions of stannous chloride and Tc-99m were added. Plasma (P) and red blood cells (RBC) were isolated, precipitated, and centrifuged, and soluble (SF) and insoluble (IF) fractions were isolated. The results show that the highest concentration (100%) of bifl orin is able to reduce the uptake of Tc-99m (%ATI) on RBC and the fi xation on IF-P. To study the infl uence of bifl orin on 99mTc lymphocyte labeling, human blood was submitted to a technique with Ficoll-Hypac and centrifuged, and white cells were isolated. Lymphocytes (2.5 mL; 1.0 x 10 6 cells/mL) were obtained and a 0.2 mL solution was incubated with bifl orin (0.1 mL). Solutions of stannous chloride and 99mTc were added. Lymphocytes were separated and the %ATI bound in these cells was evaluated. A reduction in %ATI (from 97.85 ± 0.99 to 88.86 ± 5) was observed for RBC and for IF-P (73.24 ± 5.51 to 20.72 ± 6.95). In this case the results showed no decrease in %ATI for the lymphocytes with bifl orin.

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Efeito de um extrato de Hipérico (Hypericum perforatum) na marcação in vitro de elementos sangüíneos com tecnécio-99m e na biodisponibilidade do radiofármaco pertecnetato de sódio em ratos Wistar

Cited by 9 publications

References 17 publications

Effect of an extract of Aloe vera on the biodistribution of sodium pertechnetate (Na99mTcO4) in rats

Effect of an extract of Aloe vera on the biodistribution of sodium pertechnetate (Na99mTcO4) in rats

Effect of medicinal plants on the parasitemia of Trypanosoma cruzi and on the biodistribution of sodium pertechnetate (Na99mTcO4)

Influence of biflorin on the labelling of red blood cells, plasma protein, cell protein, and lymphocytes with technetium-99m: in vitro study

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