Led enhancement in mitochondrial oxidative phosphorylation for hepatectomized rats

Abstract

Recently, the LED (light emitting diode) developed by the Optics Group of IFSC-USP has been used instead of laser for the treatment of skin tumors by the PDT (Photodinamic Therapy) because of its low operational cost compared to the use of a laser. In this paper we investigate the effect of LED light on oxidative phosphorylation during liver regeneration after partial hepatectomy. Twenty-four male Wistar rats (250 g) were kept in identical housing units on a 12-hour light/12 hour dark cycle. The LED 10 group was exposed to LED at 638 nm (10 J/cm 2 for 3 minutes). Seventy percent partial hepatectomy was performed in the LED 10 and HPC (Partial Hepatectomy-Control). A sham-operated group (C) was used for control. Twenty four hours after the procedure, LED 10, HPC and control animals were sacrificed. Samples of liver tissue were used for the mitochondrial respiration assay. Statistical comparisons of the groups were performed by analysis of variance (ANOVA), followed by the Bonferroni post-test. Probability values less than 0.05 were considered to be statistically significant. the phosphorylation index (FI) for the LED 10 group was higher than that for the HPC group and for the sham group (p<005). The FI for the HPC group was higher than that for the sham group (p<005). The values of the ADP:O ratio for the three groups, which did not differ significantly from one another (p > 0.05). In the present study we noted an effective interaction between LED light and hepatic mitochondria, with an increased phosphorylation rate for the latter. Available from: URL: http://www.scielo.br/acb

Low‐level Laser Therapy Ameliorates CCl4‐induced Liver Cirrhosis in Rats

“…Considering that AST is located predominantly in the mitochondria, the present results may suggest that part of the beneficial effects of LLLT on liver function could be attributed to improved mitochondrial function. In line with this concept, previous studies have shown that LLLT and LED therapy may improve mitochondrial function in hepatectomized rats . The fact that LLLT reduced the levels of liver enzymes such as AST, AP, GGT and LDH and also the total protein and globulin levels is extremely relevant and points out the possible mechanism of action of LLLT in CCl 4 ‐induced hepatic cirrhosis.…”

Análise termográfica da região de masseter após irradiação com laser ou LED – estudo clínico

“…A terapia com LED de baixa intensidade tem sido reportada como análoga da TLBI 8 , pois exerce efeito sobre a microcirculação, levando a uma vasodilatação 9 . Ocorrendo a vasodilatação, a quantidade de plasma que migra para o interstício é maior, deixando o sangue mais viscoso, o que torna a circulação mais lenta 10 .…”